中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (53): 9965-9967.doi: 10.3969/j.issn.1673-8225.2010.53.021

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

建立小鼠腹腔心脏移植模型的方法改进　

汪向飞，张国超，周汉新   

  1. 暨南大学附属第二临床医学院(深圳市人民医院)临床医学研究中心，广东省深圳市        518020
  • 出版日期:2010-12-31 发布日期:2010-12-31
  • 作者简介:汪向飞， snoopywxf@ yahoo.com.cn
  • 基金资助:
    国家自然科学基金(30772042)项目。

Improved establishment of a heterotopic heart transplantation model in mice

Wang Xiang-fei, Zhang Guo-chao, Zhou Han-xin   

  1. Clinical Medical Research Center, Second Clinical College of Jinan University (Shenzhen People’s Hospital), Shenzhen  518020, Guangdong Province, China
  • Online:2010-12-31 Published:2010-12-31
  • About author:Wang Xiang-fei, Clinical Medical Research Center, Second Clinical College of Jinan University (Shenzhen People’s Hospital), Shenzhen 518020, Guangdong Province, China snoopywxf@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30772042*

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摘要：

背景：小鼠心脏移植模型目前有颈部移植和腹腔移植。由于颈部空间狭窄，颈总动脉相对细小，移植心脏易于周围组织粘连，限制移植心脏搏动，不利于移植物长期观察。腹主动脉相对较粗，易于吻合，且长期血管通常率高，能够对移植心脏长期观察进行慢性移植物血管病变研究。
目的：对小鼠腹部心脏移植的技术方法进行改进，为进行移植免疫学研究提供动物模型。
方法：采用供心主动脉与受体腹主动脉、供心肺动脉与受体下腔静脉端侧吻合方法对小鼠进行腹部心脏移植，按随机数字表法将小鼠分为3组，同系移植组为同种同基因C57→C57，同种移植组为同种异基因Babl/c→C57，抗CD45RB mAb组为抗CD45RB mAb 200 μg腹腔注射Babl/c→C57。以供心搏动有力，且小鼠存活72 h以上视为移植成功。设移植后40 d为观察终点。
结果与结论：共实施手术36例次，受体存活30只，成功率为83.3%。其中受体准备时间为(15±2) min，供心摘取时间为(8±1) min，血管吻合时间为(33±2) min。抗CD45RB mAb腹腔注射组小鼠移植心脏存活时间较同种移植组明显延长(P=0.001)。提示所建立的小鼠心脏移植模型稳定可靠，可用于移植免疫学方面的研究。抗CD45RB mAb可明显延长移植心脏存活时间。

关键词: 心脏移植, 抗CD45RB mAb, 免疫耐受, 存活时间, 方法改进

Abstract:

BACKGROUND: Cervical or intraperitoneal transplantation is used in establishing heart transplantation. However, the transplanted heart is prone to adhere to surrounding tissues and restrict heart beat due to the stegnotic cervical space. Abdominal aorta is relative easily for anastomosing and has high vascular pass rate, which can be used for long-term observation of chronic graft vascular lesion.
OBJECTIVE: To explore an improved technique of mouse ventral heterotopic heart transplantation and construct an animal model for the study of transplantation immunity.
METHODS: The donor heart aorta and the recipient ventral aorta, the donor pulmonary artery and the recipient inferior caval vein, were anastomosed by using the end-to-side suture technique respectively. All animals were divided into 3 groups. Isograft group: C57 to C57 mice transplantation; Allograft group; Babl/c to C57 mice transplantation; Anti-CD45RB mAb group: Anti-CD45RB mAb (200 μg) were intraperitoneal injected into Babl/c to C57 mice. The transplanted heart beat strongly and the mouse survived for over 72 hours were considered signs of model success. The observation lasted for 40 days after transplantation.
RESULTS AND CONCLUSION: The successful rates were 83.3% (30/36). In experiments, the recipient preparation time was (15±2) minutes, harvesting time of donors was (8±1) minutes, and the recipient operation time was (33±2) minutes. In CD45RB mAb group, the average of the survival of heart allograft had a significant difference compared with those of the no therapy group (P=0.001). The animal model is stable and can be used for the study of transplantation immunity. A single injection of CD45RB mAb in mice can induce stable long-term acceptance of heart allografts.

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