中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (53): 9968-9972.doi: 10.3969/j.issn.1673-8225.2010.53.022

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

大鼠胰岛细胞经门静脉肝内移植模型的建立

杨顺良1，吴志贤1，叶永峰2，蔡锦全1，王庆华1，黄梁浒1，林国章1，周  浩1，谭建明1   

  1. 1解放军南京军区福州总医院泌尿外科，福建省福州市  350025；2解放军第184医院泌尿外科, 江西省鹰潭市  335000
  • 出版日期:2010-12-31 发布日期:2010-12-31
  • 通讯作者: 谭建明，主任医师，教授，解放军南京军区福州总医院泌尿外科，福建省福州市 350025
  • 作者简介:杨顺良★，男，1965年生，汉族，1987年解放军第二军医大学毕业，硕士，副主任医师，主要从事器官移植的基础与临床研究。 ysliang@medmail.com.cn
  • 基金资助:

    南京军区2007年度医学科技创新经费资助项目；福建省科技计划重大项目(2009Y4001)“糖尿病干细胞治疗关键技术研究与应用”；福建省科技创新平台计划项目(2008J0106)“福建省移植生物学重点实验室”。

Intrahepatic islet transplantation through the portal vein in rats

Yang Shun-liang1, Wu Zhi-xian1, Ye Yong-feng2, Cai Jin-quan1, Wang Qing-hua1, Huang Liang-hu1, Lin Guo-zhang 1, Zhou Hao1,  Tan Jian-ming1   

  1. 1 Department of Urinary Surgery, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou   350025, Fujian Province, China; 2 Department of Urinary Surgery, the 184 Hospital of Chinese PLA, Yingtan   335000, Jiangxi Province, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Tan Jian-ming, Chief physician, Professor, Department of Urinary Surgery, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China
  • About author:Yang Shun-liang★, Master, Associate chief physician, Department of Urinary Surgery, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China ysliang@medmail.com.cn
  • Supported by:

    the Medical Science and Technology Innovation Foundation of Nanjing Military Area Command of Chinese PLA in 2007*; the Major Program of Science and Technology Plan of Fujian Province, No. 2009Y4001*; the Science and Technology Innovation Platform Plan Program of Fujian Province, No. 2008J0106*

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被引次数

4

摘要：

背景：由于肝脏既是胰岛素的作用部位，又是相对的免疫特惠区，排斥反应小，肝窦及其小静脉结构还有利于胰岛的居留和生长，可供移植容积大，有利于胰岛的生存，因此肝脏是一个较为理想的移植部位。
目的：建立一种经门静脉胰岛细胞肝内移植治疗SD大鼠1型糖尿病的动物模型。
方法：采用文献方法制备SD大鼠胰岛细胞。以腹腔注射链尿佐菌素诱导建立SD大鼠1型糖尿病模型，随机数字表法分为2组：实验组经门静脉主干穿刺输注SD大鼠胰岛1 000 胰岛当量1.5 mL；对照组经门静脉主干穿刺输注无血清1640液1.5 mL。术后未给予免疫抑制剂，观察两组大鼠出血量、血糖、胰岛素水平及肝脏组织形态学变化。 
结果与结论：所有大鼠均移植成活，门静脉穿刺一次成功率90%，出血量< 0.5 mL。胰岛移植大鼠血糖降为正常的时间1~6(3.7±1.7) d，移植胰岛存活时间为2~15(8.4±4.1) d。术后2周内实验组大鼠空腹血清胰岛素水平明显高于对照组(P < 0.05，P < 0.01)。病理结果证实，移植大鼠肝细胞形态、肝小叶结构均正常，肝实质未见坏死灶，血管内无血栓形成；门静脉主干未见狭窄，亦未发生感染，说明胰岛细胞在肝窦内存活并能发挥功能。证实大鼠胰岛经门静脉主干穿刺输注肝内移植是胰岛移植基础研究的理想模型。

关键词: 糖尿病, 胰岛, 移植, 门静脉, 大鼠, 组织细胞移植

Abstract:

BACKGROUND: The liver is commonly used ideal site because the liver is not only the site of insulin action, but also a relative immunologically privileged site. Furthermore, the volume of liver is big enough for transplantation; the construction of sinus hepaticus and vein is profited for dwell and growth of islet cell.
OBJECTIVE: To investigate the method of intrahepatic islet transplantation through portal vein in Sprague Dawley rats with type I diabetes.
METHODS: Islet cells of Sprague Dawley rats were prepared by methods of a previous study. Type I diabetes mellitus was induced with streptozotocin in Sprague Dawley rats via intraperitoneal injection. The rats were divided into two groups. 1 000 IEQ islets (1.5 mL) were infused into the liver of Sprague Dawley rats by the main portal vein puncture as experimental group, and serum-free 1640 medium (1.5 mL) was infused as control group. No immunosuppressive drug was administered after operation. Bleeding volume, blood glucose, insulin level and histomorphological changes in the liver were observed.
RESULTS AND CONCLUSION: All rats survived. Success rate of portal vein puncture was 90% with bleeding volume less than 0.5 mL. Duration of normal blood glucose after operation in experimental group was 1 to 6 days (3.7 ± 1.7) days. The islet graft survival duration was 2 to 15 days (8.4 ± 4.1) days. Serum insulin levels in experimental group were significantly higher compared with control group within 2 weeks post-operative (P < 0.05, P < 0.01). The pathologic examination has confirmed that the morphous of hepatocyte and structure of hepatic lobule were normal. There was no necrosis or infection lesion in liver parenchyma and thrombopoiesis. The stenosis of main portal vein did not occur. Islet graft was pathologically viable and functioning in hepatic sinusoids. These have suggested that intrahepatic islet transplantation through the main portal vein in rats is an acceptable method.

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