中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (53): 9941-9945.doi: 10.3969/j.issn.1673-8225.2010.53.015

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

冻存卵巢组织移植后抗损伤处理对移植结局的影响

张  爽，王  冬，姜英雁，任宝红，段春凤　　   

  1. 吉林医药学院附属四六五医院妇产科，吉林省吉林市  132013
  • 出版日期:2010-12-31 发布日期:2010-12-31
  • 通讯作者: 王冬，硕士生导师，教授，主任医师，吉林医药学院附属四六五医院妇产科，吉林省吉林市132013 Wangdong1960@126.com
  • 作者简介:张爽★，女， 1982年生，吉林省吉林市人，汉族，吉林医药学院附属医院在读硕士，医师，主要从事妇科肿瘤方面的研究。 ciea_liu@126.com
  • 基金资助:

    吉林省科技发展计划项目(200705415)，卵巢组织冷冻方法优化及抗损伤处理对移植结局影响的研究。

Anti-injury treatment effects on transplant outcomes following cryopreserved ovarian tissue transplantation

Zhang Shuang, Wang Dong, Jiang Ying-yan, Ren Bao-hong, Duan Chun-feng   

  1. Department of Gynaecology and Obstetrics, the 465 Hospital Affiliated to Jilin Medical College, Jilin  132013, Jilin Province, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Wang Dong, Master’s supervisor, Professor, Chief physician, Department of Gynaecology and Obstetrics, the 465 Hospital Affiliated to Jilin Medical College, Jilin 132013, Jilin Province, China wangdong1960@126.com
  • About author:Zhang Shuang★, Studying for master’s degree, Physician, Department of Gynaecology and Obstetrics, the 465 Hospital Affiliated to Jilin Medical College, Jilin 132013, Jilin Province, China ciea_liu@126.com
  • Supported by:

     the Science and Technology Development Project of Jilin Province, No. 200705415*

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摘要：

背景：卵巢组织移植是无血管吻合移植，改善卵巢组织对移植后损伤的耐受力是提高冻存后移植卵巢组织卵泡存活和发挥功能的关键环节。
目的：比较冻存后卵巢组织自体异位移植后，应用血管内皮生长因子、维生素E、黄芪抗损伤处理对卵巢组织形态和功能恢复的影响。
方法：将成年雌性Wistar大鼠随机数字表法分为4组：正常对照组、去势组、自体新鲜卵巢组织移植组(卵巢组织未冻存，直接移植)、冷冻保存卵巢组织移植组。冷冻保存卵巢组织移植组移植后分3组干预：未经抗损伤处理组，血管内皮生长因子、维生素E、黄芪联合抗损伤组，血管内皮生长因子、维生素E联合抗损伤组。移植2个月后检测血清雌二醇水平，苏木精-伊红染色后观察卵泡的形态及正常卵泡数量。
结果与结论：应用血管内皮生长因子、维生素E、黄芪联合抗损伤组较未用药物组血清雌二醇水平升高，初级卵泡数量增加，且差异显著(P < 0.05)，即抗损伤药物对卵巢形态和功能的恢复有作用。血管内皮生长因子、维生素E、黄芪联合抗损伤组血清雌二醇水平、初级卵泡数量略高于血管内皮生长因子、维生素E联合抗损伤组，但差异无显著性意义。尚不能认为加用黄芪抗损伤处理效果更佳。提示自体异位移植后应用血管内皮生长因子、维生素E、黄芪抗损伤处理对卵巢形态和功能的恢复均有作用。

关键词: 卵巢组织, 血管内皮生长因子, 维生素E, 黄芪, 组织移植

Abstract:

BACKGROUND: Transplantation of ovarian tissue is performed without vascular reanastomosis. To ameliorate the tolerance of ovarian tissue to injuries is critical for follicular survival and for exerting functions of the cryopreserved graft.
OBJECTIVE: To compare the effects of vascular endothelial growth factor (VEGF), vitamin E, milkvetch root anti-injury treatment on morphology and function of freeze ovarian tissue after cryopreserved ovarian tissue autologous heterotopic transplantation.
METHODS: Adult female Wistar rats were randomly assigned to four groups: normal control group, ovariectomy group, autologous fresh ovarian tissue transplantation group (non-cryopreserved ovarian tissue, direct transplantation) and cryopreserved ovarian tissue transplantation group. The cryopreserved ovarian tissue transplantation group was divided into three subgroups: non-anti-injury treatment group, VEGF + vitamin E + milkvetch root group and VEGF + vitamin E anti-injury group. At 2 months posttransplantation, serum estradiol (E2) level was detected. Following hematoxylin-eosin staining, follicular morphology and number of normal ovarian follicle were observed.
RESULTS AND CONCLUSION: E2 levels were increased in the VEGF + vitamin E + milkvetch root group compared with no drug group, and number of primary follicle was increased (P < 0.05). The anti-injury drug affected ovary shape and functional recovery. E2 levels and number of primary follicle were greater in the VEGF + vitamin E + milkvetch root group compared with VEGF + vitamin E anti-injury group, but no significant difference was found. We could not confirm that the milkvetch root anti-injury treatment obtained good outcomes. These indicate that VEGF + vitamin E + milkvetch root anti-injury treatment exerts effects on the recovery of ovary shape and function following autologous heterotopic transplantation.

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