中国组织工程研究

中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (10): 1495-1499.doi: 10.3969/j.issn.2095-4344.3067

• 组织工程骨材料Tissue-engineered bone • 上一篇    下一篇

羟基磷灰石/明胶支架复合骨髓间充质干细胞和脐静脉内皮细胞修复兔颅骨缺损

范海霞1，谈庆坤1，王  宏2，程焕芝1，刘  雪1，Ching-chang Ko3，耿海霞1   

  1. 1济宁医学院，山东省济宁市  272067；2潍坊医学院，山东省潍坊市   261053；3美国北卡罗莱纳大学UNC牙学院，美国罗莱纳州  27616
  • 收稿日期:2020-03-27 修回日期:2020-04-01 接受日期:2020-05-09 出版日期:2021-04-08 发布日期:2020-12-17
  • 通讯作者: 耿海霞，教授，济宁医学院，山东省济宁市 272067
  • 作者简介:范海霞，女，1981年生，山东省济宁市人，汉族，2016年哈尔滨医科大学毕业，博士，副教授，主要从事口腔癌发生机制和治疗方面的研究。
  • 基金资助:
    山东省自然科学基金项目(ZR2017LH044)；济宁医学院国家自然科学基金培育项目(2016-09)；济宁医学院大学生创新训练计划项目(201910443003)

Rabbit skull defects repaired by the hydroxyapatite/geltin scaffold combined with bone marrow mesenchymal stem cells and umbilical vein endothelial cells

Fan Haixia1, Tan Qingkun1, Wang Hong2, Cheng Huanzhi1, Liu Xue1, Ching-chang Ko3, Geng Haixia1    

  1. 1Jining Medical University, Jining 272067, Shandong Province, China; 2Weifang Medical University, Weifang 261053, Shandong Province, China; 3School of Dentistry, University of North Carolina at Chapel Hill, Chapel Hill 27616, NC, USA
  • Received:2020-03-27 Revised:2020-04-01 Accepted:2020-05-09 Online:2021-04-08 Published:2020-12-17
  • Contact: Geng Haixia, Professor, Jining Medical University, Jining 272067, Shandong Province, China
  • About author:Fan Haixia, MD, Associate professor, Jining Medical University, Jining 272067, Shandong Province, China
  • Supported by:
    Shandong Natural Science Foundation, No. ZR2017LH044; Natural Science Foundation of Jining Medical University, No. 2016-09; College Students’ Innvovative Training of Jining Medical University, No. 201910443003 

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摘要：

文题释义：
骨髓间充质干细胞和脐静脉内皮细胞：均因具有多向分化的潜能，已成为目前构建组织工程骨良好的种子细胞。骨髓间充质干细胞和脐静脉内皮细胞复合培养，不仅能促进成骨细胞的增殖活性，亦能更好地促进组织工程骨的血管化，有助于增强组织工程骨修复骨缺损的能力。
羟基磷灰石纳米晶体：羟基磷灰石为人体骨骼和牙齿的主要无机成分，是种理想的骨替代材料。纳米晶体表面具有较高的表面积和孔隙率，有利于细胞接种、迁移和增殖，并且其仿生化的微环境可调控细胞与细胞、细胞与基质之间的相互作用，调节细胞的生物学行为。

背景：羟基磷灰石/明胶复合材料具有良好的生物相容性及骨诱导性，可作为组织工程支架用于修复骨缺损。
目的：观察3D打印羟基磷灰石/明胶支架复合骨髓间充质干细胞和脐静脉内皮细胞修复兔颅骨缺损的效果。
方法：取9只雄性新西兰白兔，建立直径约0.8 cm的颅骨缺损模型，分3组处理：空白组不做处理，对照组植入3D打印羟基磷灰石/明胶支架，实验组植入3D打印羟基磷灰石/明胶支架与骨髓间充质干细胞和脐静脉内皮细胞复合物，每组3只。术后第8周，对各组白兔进行颅骨锥形束CT扫描与颅骨缺损部位组织学观察。动物实验获得济宁医学院伦理委员会批准。
结果与结论：①锥形束CT扫描：空白组可见明显的骨缺损，缺损区域与周围正常骨组织边缘有点状不透射影；对照组可见有部分新生骨形成，呈不连续状，与周围骨组织亦不连贯；实验组可见新生骨组织呈线性连续，厚度较薄，缺损区域与邻近骨组织边缘融合；②组织学观察：苏木精-伊红染色与Masson三色显示，空白组缺损区见纤维结缔组织生成及少量游离骨细胞；对照组可见少量成骨，材料边缘可见有骨基质沉积，取代材料形成骨小梁；实验组材料空间逐渐被新生骨代替，新生骨和骨小梁结构样组织充填于缺损区域；③结果表明，3D仿生打印羟基磷灰石/明胶支架复合骨髓间充质干细胞和脐静脉内皮细胞可有效促进骨组织的生长，加快骨缺损修复。

https://orcid.org/0000-0002-7683-5464 (范海霞) 

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 干细胞, 骨, 骨再生, 3D打印, 组织工程骨, 骨缺损, 骨髓间充质干细胞, 脐静脉内皮细胞

Abstract: BACKGROUND: Hydroxyapatite-geltin composite has good biocompatibility and osteoinductivity, and can used be as tissue engineering scaffold to repair bone defects.
OBJECTIVE: To observe the effect of three-dimensional (3D) printed hydroxyapatite/gelatin scaffold combined with bone marrow mesenchymal stem cells and umbilical vein endothelial cells in repairing rabbit skull defects.
METHODS: Nine male New Zealand white rabbits were taken to establish a skull defect model with a diameter of approximately 0.8 cm, and randomly divided into three groups: blank group: no any treatment; control group: only 3D printed hydroxyapatite-geltin scaffold; experimental group: 3D printed hydroxyapatite-geltin scaffold and bone marrow mesenchymal stem cells and bone marrow mesenchymal stem cells complex group, with three rabbits in each group. At 8 weeks after the operation, CT scan of the skull pyramid beam and histological observation of the skull defect were performed on the white rabbits of each group. Animal experiments were approved by the Ethics Committee of Jining Medical College.
RESULTS AND CONCLUSION: (1) CT scan of pyramidal tract: Blank group showed obvious bone defects, and the defect area was slightly radiopaque with the edges of the surrounding normal bone tissue. Control group showed some new bone formation, which was discontinuous and inconsistent with the surrounding bone tissue. Experimental group showed that the new bone tissue was linear and continuous; the thickness was thin; and the defect area merged with the adjacent bone tissue edge. (2) Histological observation: Hematoxylin-eosin staining and Masson trichrome staining showed that fibrous connective tissue formation and a small amount of free bone cells were seen in the defect area of the blank group. A small amount of bone formation was seen in the control group. Bone matrix was deposited at the edge of the material, replacing the material to form small bone trabeculae. The material space of the experimental group was gradually replaced by new bone, and the defect area was filled with new bone and trabecular bone structure-like tissue. (3) The results show that the 3D bionic printing hydroxyapatite-geltin scaffold combined with bone marrow mesenchymal stem cells and bone marrow mesenchymal stem cells can effectively promote the growth of bone tissue and accelerate the repair of bone defects. 

Key words: stem cells">, bone">, bone regeneration">, 3D printing">, tissue engineering bone">, skull defect">, bone marrow mesenchymal stem cells">, umbilical vein endothelial cells

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