中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (34): 5538-5544.doi: 10.3969/j.issn.2095-4344.2017.34.021

• 生物材料综述 biomaterial review • 上一篇    下一篇

短肽水凝胶RADA16结构特征及在生物医学中的应用研究与进展

张  玲1,余丽梅1,2,刘燕飞1,2 
  

  1. 遵义医学院附属医院,1贵州省细胞工程重点实验室,2贵州省生物治疗人才基地,贵州省遵义市  563003
  • 收稿日期:2017-09-04 出版日期:2017-12-08 发布日期:2018-01-04
  • 通讯作者: 刘燕飞,博士,副研究员,硕士生导师,遵义医学院附属医院,贵州省细胞工程重点实验室,贵州省生物治疗人才基地,贵州省遵义市 563003
  • 作者简介:张玲,女,四川省成都市人,汉族,遵义医学院在读硕士,主要从事医用水凝胶与羊膜干细胞研究。
  • 基金资助:

    贵州省教育厅自然科学研究项目[黔教合KY字[2015]418];贵州省科技创新人才团队建设项目[黔科合人才团队[2016]5614号]

Characteristics of self-assembling peptide hydrogel RADA16 and its application in biomedical field 

Zhang Ling1, Yu Li-mei1, 2, Liu Yan-fei1, 2
  

  1. 1Key Laboratory of Cell Engineering of Guizhou Province, 2Biological Treatment Talent Base of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China
  • Received:2017-09-04 Online:2017-12-08 Published:2018-01-04
  • Contact: Liu Yan-fei, Ph.D., Associate researcher, Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China; Biological Treatment Talent Base of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China
  • About author:Zhang Ling, Studying for master’s degree, Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China
  • Supported by:
    the Natural Science Research Project of Education Department of Guizhou Province, No. Qian-jiao-he-ke-zi [2015]418; Guizhou Province Science and Technology Innovation Talent Team Construction Project, No. [2016]5614

摘要:

文章快速阅读:

 

文题释义:
自组装短肽水凝胶:在特定环境下(离子浓度、温度、pH值、光及酶等),通过非共价键作用(氢键、范德华力、静电作用、疏水作用、π-π堆积等)形成特定二级结构(如α-螺旋及β-折叠等),最终自组装成含水量≥ 99% 的超分子纳米纤维短肽水凝胶。
RADA16水凝胶研究意义:自组装短肽RADA16形成了纳米纤维网状结构,孔径为5-200 nm,纤维直径8- 10 nm,具有类似体内细胞外基质结构;皆由简单的氨基酸成分组成,结构稳定,无毒性,降解产物不产生免疫反应等优秀特质,使其能够相对真实地模拟细胞外基质成分,为细胞生长提供必要的三维环境。
 
背景:与传统生物材料相比,自组装短肽RADA16具有含水量高、结构稳定、生物相容性好及降解产物无毒等优点,近几年在细胞三维培养、组织修复、快速止血及药物/蛋白缓释等生物医学领域具有良好的应用前景。
目的:综述短肽水凝胶RADA16基本结构与性能特点及其在生物医学领域的研究进展。
方法:应用计算机检索CNKI,Medline及PubMed数据库2005年至2017年关于自组装短肽水凝胶RADA16的文献,中文检索关键词为“自组装短肽水凝胶;RADA16;支架;组织修复;止血;药物/蛋白缓释”,英文检索关键词为“self-assembling peptide hydrogel;RADA16;scaffold;tissue repair;hemostasis;drug/protein release ”。
结果与结论:生理介质或特定盐溶液条件下,自组装肽类分子能够自发形成独特的β-折叠构型,并自组装成纳米纤维,作为新型组织工程支架材料不仅解决了细胞-材料界面不相容的问题,还具备有效模拟细胞外基质、提高细胞生物活性和维持三维环境等优势,RADA16自组装短肽及其衍生肽在三维细胞培养、组织修复、止血以及药物/蛋白缓释等方面展示了良好的开发应用前景,同时也面临诸多挑战,例如如何与新型生物高分子的整合及如何控制损伤靶点等。

关键词: 生物材料, 药物控释材料, 自组装短肽水凝胶, RADA16, 支架, 组织修复, 止血, 药物/蛋白缓释, 组织工程

Abstract:

BACKGROUND: Compared with traditional biological materials, self-assembling peptide RADA16 has gained much attention in biomedical fields such as three-dimensional cell culture, tissue repair, hemostasis and  drug/protein release for its high water content, structural stability, good biocompatibility and nontoxic degradation products.
OBJECTIVE: To review the basic structure and properties of self-assembling peptide RADA16 and the latest progress in biomedical research.
METHODS: CNKI, Medline and PubMed databases were retrieved by using computer to search relevant articles about self-assembling peptide RADA16 published from 2005 to 2017. The key words were “self-assembling peptide hydrogel; RADA16; scaffold; tissue repair; hemostasis; drug/protein release” in Chinese and English, respectively.
RESULTS AND CONCLUSION: Self-assembling peptide molecules can spontaneously form unique β-strand structures and self-assembly into nanofibers under physiological media or salt solution. As a new scaffold material for tissue engineering, it not only solves the problem of incompatibility between cells and material interface, but also has the advantages of simulating the extracellular matrix effectively, enhancing cell biological activity and maintaining three-dimensional environment. The self-assembling peptide RADA16 and its derivatives not only show good prospects for development and application in three-dimensional cell culture, tissue repair, hemostasis, and drug/protein release, but also face many challenges, such as how to integrate the self-assembling peptide with bio-macromolecular material, and how to control the damage to a target.

Key words: Tissue Engineering, Biocompatible Materials, Stents

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