中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (29): 4600-4604.doi: 10.3969/j.issn.2095-4344.2017.29.002

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

甲状旁腺激素对大鼠骨髓间充质干细胞钙离子的影响

陈玉书1，张燕红2，张姝江1，白 波1，陈 艺1   

  1. 1广州医科大学附属第一医院广东省骨科矫形与植入材料重点实验室，广东省广州市 510120；2南方医科大学顺德医院骨科，广东省顺德市  528300
  • 修回日期:2017-05-04 出版日期:2017-10-18 发布日期:2017-11-08
  • 通讯作者: 白波，主任医师，教授，广州医科大学附属第一医院骨科，广东省广州市 510120
  • 作者简介:陈玉书，男，1982年生，广东省广州市人，汉族，主要从事骨关节损伤研究。

Effects of parathyroid hormone on calcium ions in rat bone marrow mesenchymal stem cells

Chen Yu-shu1, Zhang Yan-hong2, Zhang Shu-jiang1, Bai Bo1, Chen Yi1   

  1. 1Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong Province, China; 2Department of Orthopaedic Surgery, Shunde Hospital of Southern Medical University, Shunde 528300, Guangdong Province, China
  • Revised:2017-05-04 Online:2017-10-18 Published:2017-11-08
  • Contact: Bai Bo, Chief physician, Professor, Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong Province, China
  • About author:Chen Yu-shu, Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong Province, China

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摘要：

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文题释义：
甲状旁腺激素：是由甲状旁腺主细胞合成与分泌的多肽类激素，具有分解代谢和合成代谢双重作用的G 蛋白偶联受体信号蛋白，可通过靶器官表面的特异性受体激活一系列生理生化反应调节钙的动态平衡。目前已有文献报道，甲状旁腺激素可明显增加骨髓间充质干细胞增殖率，减少其衰老与凋亡，维持其基因组的完整性。
分离纯化骨髓间充质干细胞的方法：有全骨髓细胞贴壁法、密度梯度离心法、流式细胞仪分离法及免疫磁珠法。密度梯度离心法、流式细胞仪分离法及免疫磁珠法虽可较快地筛选出高纯度的骨髓间充质干细胞，但会让骨髓间充质干细胞的生长外界微环境发生改变，既影响细胞的活力，也造成细胞的丢失。全骨髓贴壁培养法是基于骨髓间充质干细胞对塑料培养瓶的黏附特性而逐步把非贴壁细胞与其他杂质细胞除去的一种简单可靠的培养方法。

 

摘要
背景：甲状旁腺激素可通过靶器官表面的特异性受体激活一系列生理生化反应，调节钙的动态平衡。
目的：观察甲状旁腺激素对骨髓间充质干细胞内钙离子的影响，探讨其维持骨髓间充质干细胞体外增殖的机制。
方法：将第4代SD大鼠骨髓间充质干细胞分组培养，实验组加入含甲状旁腺激素的培养基，对照组加入常规培养基，培养10 d内，MTT法检测细胞增殖；培养7，14 d，通过激光共聚焦显微镜观察实验组及对照组细胞内钙离子荧光峰值。
结果与结论：①细胞增殖：培养第一二天，2组细胞生长活性均滞缓，3 d后进入快速增长期，在第七八天进入平台期，第10天细胞增殖能力有减弱趋势，其中对照组细胞增殖能力低于实验组(P < 0.05)；②激光共聚焦显微镜观察结果：两组于培养第7天可见胞内呈现较强的钙荧光峰值，随着诱导时间延长，14 d的细胞内钙荧光峰值出现下降，实验组培养7，14 d的细胞内的钙荧光峰值强于对照组(P < 0.001)，且实验组14 d的细胞内钙荧光峰值与对照组7 d的细胞内钙荧光峰值无差异；③结果表明：甲状旁腺激素可增加骨髓间充质干细胞内钙离子荧光峰值，维持骨髓间充质干细胞增殖功能。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0003-0972-8420(白波)

关键词: 干细胞, 骨髓干细胞, 甲状旁腺激素, 钙离子, 骨髓间充质干细胞, 大鼠, 激光共聚焦显微镜

Abstract:

BACKGROUND: Parathyroid hormone can activate a series of physiological and biochemical reactions through specific receptors on the surface of target organs, to adjust the dynamic calcium balance.
OBJECTIVE: To explore the mechanisms whereby parathyroid hormone maintains in vitro proliferation of bone marrow mesenchymal stem cells (BMSCs), by observing the effects of parathyroid hormone on calcium ions in BMSCs.
METHODS: BMSCs at passage 4 were randomly divided into two groups. Experimental group was treated with parathyroid hormone, and control group was treated with routine culture medium. During 10 days of culture, MTT assay was used to detect BMSCs proliferation. At 7 and 14 days after treatment, laser scanning confocal microscopy was used to detect fluorescence intensity of calcium ions in BMSCs in the two groups.
RESULTS AND CONCLUSION: (1) At 1 and 2 days after culture, the cell viability in the two groups was sluggish. At 3 days after culture, the cells turned into the rapid growth. At 7 and 8 days after culture, the cell growth turned into the plateau. At 10 days after culture, the cell proliferation ability had a weakening trend, which was lower in the control group than in the experimental group (P < 0.05). (2) At 7 days after culture, the fluorescence intensity of intracellular calcium ions was strong. As the induction time extended, the fluorescence intensity of intracellular calcium ions was declined at 14 days after-culture. At 7 and 14 days after culture, the fluorescence intensity of intracellular calcium ions in the experimental group was higher than that in the control group (P < 0.001). There was no difference in the fluorescence intensity of intracellular calcium ions between experimental group at 14 days and control group at 7 days. To conclude, parathyroid hormone could increase the fluorescence intensity of calcium ions in BMSCs, and function to maintain the BMSCs proliferation.  

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Mesenchymal Stem Cells, Teriparatide, Cell Proliferation, Tissue Engineering

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