中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (22): 3589-3594.doi: 10.3969/j.issn.2095-4344.2017.22.024

• 生物材料综述 biomaterial review • 上一篇    下一篇

3D生物打印组织器官及其在口腔医学中的研究与进展

林雨恒,王旭东,沈国芳
  

  1. 上海交通大学医学院附属第九人民医院口腔颅颌面科,上海市口腔医学重点实验室,上海市  200011
  • 收稿日期:2017-03-09 出版日期:2017-08-08 发布日期:2017-09-01
  • 通讯作者: 王旭东,教授,主任医师,上海交通大学医学院附属第九人民医院口腔颅颌面科,上海市口腔医学重点实验室,上海市 200011
  • 作者简介:林雨恒,男,1991年生,四川省资阳市人,汉族,上海交通大学口腔医学院在读硕士,主要从事颅颌面畸形的临床和基础研究。
  • 基金资助:

    国家自然科学基金(81271122,81570947);上海高校高峰高原学科建设项目;上海高校创新团队计划

3D bioprinting of tissues and organs and its application in oral medicine

Lin Yu-heng, Wang Xu-dong, Shen Guo-fang
  

  1. Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
  • Received:2017-03-09 Online:2017-08-08 Published:2017-09-01
  • Contact: Wang Xu-dong, Professor, Chief physician, Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
  • About author:Lin Yu-heng, Studying for master’s degree, Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
  • Supported by:
     the National Natural Science Foundation of China, No. 81271122, 81570947; Shanghai Summit & Plateau Disciplines; and the ?Program for Innovation Research Teams of the Shanghai Municipal Education Commission

摘要:

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文题释义:
3D生物打印:是将3D打印技术与计算机科学、细胞生物学及组织工程技术相结合而形成的具有广阔应用潜力的打印技术,其原理是利用生物打印机将不同类型的生物材料按预先设计的三维结构逐层打印,最终形成具有生物活性功能的组织与器官。
熔融沉积制造:作为3D打印技术的一种,通过高温将材料融化为液态,以打印喷头将材料挤出且固化后形成三维立体模型,尤其适合于受力及负荷较大如骨或软骨等部位的支架生物打印。Goh等通过熔融沉积聚己内酯以保存牙槽嵴位点,Reichert等利用聚己内酯制造含有20%磷酸三钙微粒的柱形支架,并与重组人骨形态发生蛋白7相结合,以促进骨形成。
 
背景:3D生物打印已被用于研究打印多种组织,包括皮肤、血管、肝脏、软骨、心脏、耳、脂肪等。
目的:综述3D生物打印在不同组织器官中的应用及其在口腔医学中的研究进展。
方法:应用计算机检索PubMed数据库、中国知网数据库2006至2016年发表的有关3D生物打印在组织器官及口腔医学中的应用的研究,检索词为“3D bioprinting,3D printing,organs,tissues,tissue engineering,stomatology,oral,cranio-maxillofacial;3D生物打印,器官,组织,组织工程,口腔医学,口腔颅颌面”。
结果与结论:目前,已有相当数量的研究利用增材制造原理打印不同组织器官的3D生物支架,但直接的细胞生物打印技术尚处于早期阶段,尽管3D生物打印已被用于研究打印多种组织包括皮肤、血管、肝脏、软骨、心脏等,但还未有成功的具有相关活性功能的3D生物细胞打印组织进入临床应用,并且在口腔医学中,绝大部分研究仍停留于打印生物支架阶段。由于人类机体结构及各组织器官功能表达的复杂性,未来可能仍需要很长一段时间对打印细胞的来源、生物支架材料、细胞外基质类型及3者之间如何更好的相互作用等方面进行不断探索。

关键词: 生物材料, 口腔生物材料, 3D生物打印, 3D打印, 器官, 组织, 口腔医学, 口腔颅颌面, 国家自然科学基金

Abstract:

BACKGROUND: 3D bioprinting has been used to print a variety of tissues including skin, vascular tissues, liver,cartilage, cardiac tissues, ear, and adipose tissues.
OBJECTIVE: To review the application of 3D bioprinting technology in printing tissues or organs and its research progress in oral medicine.
METHODS: A computer-based retrieval of PubMed and CNKI was performed to search articles concerning the application of 3D bioprinting technology in printing tissues, organs and stomatology which were published from 2006 to 2016, using the keywords of “3D bioprinting, 3D pinting, organs, tissues, tissue engineering, stomatology, oral, cranio-maxillofacial” in English and Chinese, respectively.
RESULTS AND CONCLUSION: Direct cell-bioprinting is still in its early stage, even though 3D bioprinting has been applied to print various tissues such as skin, vascular tissues, liver, cartilage and cardiac tissues, etc. Although additive manufacturing technology has been widely used for printing different scaffolds of tissues and organs nowadays, there are no successful bioprinted tissues with biological functions in clinical practice. Even in oral medicine, most of the studies still remain in the stage of printing biological scaffolds. It is a long way to solve the problems such as source of cells, materials of biological scaffolds and types of extracellular matrix in the future, due to the complexity of human organism structure and the function expression of various tissues and organs. 

Key words: Artificial Organs, Oral Medicine, Tissue Engineering

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