中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (21): 3293-3298.doi: 10.3969/j.issn.2095-4344.2017.21.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

当归多糖对小鼠骨髓造血干细胞及基质细胞表面细胞间黏附分子1水平的影响

王改琴1，景 鹏2，贾书花1   

  1. 1长治医学院组织学与胚胎学教研室，山西省长治市  046000：2长治县人民医院急诊科，山西省长治市 046000
  • 修回日期:2017-05-20 出版日期:2017-07-28 发布日期:2017-08-02
  • 通讯作者: 贾书花，教授。长治医学院组织学与胚胎学教研室，山西省长治市 046000
  • 作者简介:王改琴，女，1980年生，山西省长治市人，汉族，2008年重庆医科大学毕业，硕士。
  • 基金资助:

    长治医学院科技创新团队项目(CX201415)；山西省高校科技开发项目(20101101)

Effect of angelica polysaccharide on bone marrow hematopoietic stem cells and intercellular adhesion molecule 1 on the surface of stromal cells in mice

Wang Gai-qin1, Jing Peng2, Jia Shu-hua1   

  1. 1Department of Histology and Embryology, Changzhi Medical College, Changzhi 046000, Shanxi Province, China; 2Emergency Department, Changzi County People’s Hospital, Changzhi 046000, Shanxi Province, China
  • Revised:2017-05-20 Online:2017-07-28 Published:2017-08-02
  • Contact: Jia Shu-hua, Professor, Department of Histology and Embryology, Changzhi Medical College, Changzhi 046000, Shanxi Province, China
  • About author:Wang Gai-qin, Master, Department of Histology and Embryology, Changzhi Medical College, Changzhi 046000, Shanxi Province, China
  • Supported by:

    the Innovation Team Project of Changzhi Medical College, No. CX201415; the Scientific and Technological Development Project of Shanxi Universities, No. 20101101

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554

被引次数

14

摘要：

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文题释义：
骨髓基质细胞：是造血微环境中的重要成分，包括血窦内皮细胞、巨噬细胞及网状细胞等，它不仅起支持作用，还分泌多种细胞因子，调节造血细胞的增殖和分化，产生网状纤维、粘连性糖蛋白等细胞外基质成分，有滞留造血细胞的作用。
造血干细胞：是生成各种血细胞的原始细胞，又称多能干细胞，其形态类似小淋巴细胞，胞质内除大量游离核糖体和少量线粒体外，无其他细胞器。

 

摘要
背景：有研究表明当归多糖有补血活血作用，但其如何改善骨髓造血微环境，促进造血功能成为研究问题热点之一。
目的：观察当归多糖对小鼠骨髓造血干细胞及基质细胞表面细胞间黏附分子1水平的影响。
方法：建立失血性贫血模型小鼠，随机分为3组，当归多糖低、高剂量组连续腹腔注射等量相应浓度的当归多糖4，6 mg/kg，对照组则同样时间内注射等量的生理盐水，共干预6 d。动态观察小鼠一般情况；全自动血细胞分析仪检测不同时间点小鼠外周血中红细胞的数量；采用磁珠分选和流式细胞术检测骨髓中造血干细胞的数量；MTT法检测骨髓基质细胞的增殖情况；采用流式细胞术检测骨髓基质细胞表面的细胞间黏附分子1的表达。  
结果与结论：当归多糖对失血性贫血小鼠体质量无明显影响(P > 0.05)。当归多糖低、高剂量组外周血红细胞数、骨髓CD34+，Sca-1+细胞百分数、骨髓基质细胞数量及其表面细胞间黏附分子1均明显高于对照组(P < 0.05)，其中高剂量组效果优于低剂量组。结果说明，当归多糖通过促进骨髓基质细胞增殖，增强细胞间黏附分子1的表达，进而促进造血干细胞增殖并影响其功能活动。

 

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0003-1871-0775(贾书花)

关键词: 干细胞, 骨髓干细胞, 当归多糖, 造血干细胞, 失血性贫血, 骨髓, 造血, 微环境, 基质细胞, 细胞间黏附分子1, 小鼠

Abstract:

BACKGROUND: Angelica polysaccharide has the function of enriching blood and promoting blood circulation, but how to improve the hematopoietic microenvironment and promote hematopoiesis has become one of the hot topics.

OBJECTIVE: To investigate the effect of angelica polysaccharide on Sca-1+, CD34+ hematopoietic stem cells and intercellular adhesion molecule 1 level in bone marrow stromal cells in mice.
METHODS: Mouse models of hemorrhagic anemia were made and then randomly divided them into control group, low-concentration angelica polysaccharide group and high-concentration angelica polysaccharide group. Angelica polysaccharide groups were intraperitoneally injected with the corresponding concentration of angelica polysaccharide (4, 6 mg/kg) for continuous 6 days, while the control group was injected with equal amount of normal saline. General conditions of the model mice were observed. Peripheral blood red cells were counted by automatic blood cell analyzer. Bone marrow hematopoietic stem cells were counted by magnetic bead sorting technique and flow cytometry. Proliferation of bone marrow stromal cells was detected by MTT method. Expression of intercellular adhesion molecule-1 in bone marrow stromal cells was detected by flow cytometry. 
RESULTS AND CONCLUSION: Angelica polysaccharide had no obvious effect on the body mass of hemorrhagic anemia mice (P > 0.05). The number of peripheral blood cells, the percentage of bone marrow CD34+ and Sca-1+ cells, the number of bone marrow stromal cells and the level of intercellular adhesion molecule 1 were ranked as follows: high-concentration group > low-concentration group > control group (P < 0.05). To conclude, angelica polysaccharide could promote the proliferation of bone marrow stromal cells and increase the expression of intercellular adhesion molecule 1, thereby promoting the proliferation of hematopoietic stem cells and regulating their functional activities.

Key words: Stem Cells, Tissue Engineering, Bone Marrow

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