中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (38): 6092-6097.doi: 10.3969/j.issn.2095-4344.2015.38.005

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

正畸应力刺激下纯钛微种植体周围炎模型及骨改建

张晓歌1，唐 甜2，赵志河2，丁 寅1   

  1. 1解放军第四军医大学口腔医院正畸科，军事口腔医学国家重点实验室，陕西省西安市  710032； 2口腔疾病研究国家重点实验室，四川大学华西口腔医院正畸科，四川省成都市  610041
  • 通讯作者: 赵志河，博士，教授，四川大学华西口腔医院正畸科， 四川省成都市 610041 丁寅，博士，教授，解放军第四军医大学口腔医院正畸科，陕西省西安市 610041
  • 作者简介:张晓歌，男，1975年生，河南省洛阳市人，汉族，博士，主治医师，主要从事错牙合畸形的矫治机制及微种植体的临床及基础研究。
  • 基金资助:

    中国博士后科学基金(2013M532159)；国家自然科学青年基金(10902075)

Histological changes of pure titanium micro-implant under inflammatory stimulation and orthodontic force as well as bone remodeling

Zhang Xiao-ge1, Tang Tian2, Zhao Zhi-he2, Ding Yin1   

  1. 1State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China; 
    2State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China)
  • Contact: Zhao Zhi-he, M.D., Professor, State Key Laboratory of Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China Ding Yin, M.D., Professor, State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
  • About author:Zhang Xiao-ge, M.D., Attending physician, State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
  • Supported by:

    China Postdoctoral Science Foundation, No. 2013M532159; the National Natural Science Foundation of China, No. 10902075

PDF

323

被引次数

13

摘要：

背景：种植体周围炎是影响种植体稳定性的重要因素。

目的：建立正畸应力刺激下比格犬微种植体周围炎动物模型，观察其骨改建过程。

方法：采用助攻法将纯钛微种植钉随机植入Beagle犬双侧上颌第二，三，四前磨牙的根分叉区，将一侧植入3枚丝线结扎的微种植体，在丝线结扎诱导微种植体周围炎后加力，分别在微种植体周围炎的第1，2，3，4周即刻施加100 g拉力，持续加力1个月。处死动物后制备含微种植体的不脱钙硬组织切片，光学显微镜观察微种植体周围炎发展不同阶段受到持续正畸应力作用下的种植体-骨界面的改建过程。

结果与结论：丝线颈部接扎的微种植体植入后，颈部组织内出现大量的梭形炎性细胞，随着时间的推移，炎症逐步扩散到微种植体的尖部，有大量胶原纤维，成骨活跃，出现新生骨进行改建；周围炎发生2周后扩散到微种植体尖部，出现深浅不一的新生骨小梁组成的编织骨，炎性细胞分散，可见胶原纤维吸收后留下的髓腔不规整，骨陷窝内可见排成三四层的成骨细胞，成骨活跃。说明实验成功构建了正畸应力刺激下Beagle犬纯钛微种植体周围炎模型，受力后约2周是该模型成骨的活跃期。

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 生物材料, 口腔生物材料, 正畸应力, 比格犬, 微种植体, 动物模型, 种植体周围炎, 正畸支抗, 甲苯胺蓝染色, 亚甲基蓝-ViGi 染色, 硬组织切片, 成骨细胞, 国家自然科学基金

Abstract:

BACKGROUND: Implant stability is mainly influenced by peri-implant inflammatory stimulation.

OBJECTIVE: To build a beagle model of peri-implantitis under orthodontic force and to observe the bone remodeling of the Beagle dog model.

METHODS: Micro-implants were randomly implanted into the maxillary interradicular region at the center of the mesial and distal roots of bilateral P2, P3, P4 and M1 of Beagle dogs. One side served as a loaded micro-implant with peri-implantitis under 100 g of orthodontic force at 1, 2, 3, 4 weeks of peri-implantitis, and the force lasted for 1 month. After that, the animals were killed to prepare specimens with micro-implants. Bone-to-implant contacts were calculated and histological changes of the bone interface under continuous orthodontic force at different stages of peri-implantitis were observed under light microscope.

RESULTS AND CONCLUSION: There were a large number of inflammatory cells after micro-implants were implanted with silk thread ligation to the cervical part. Over time, inflammatory cells were gradually diffused to the tip of micro-implant, and there were a great quantity of collagenous fibers, osteocytes and active bone remodeling. When the inflammation was diffused to the tip of micro-implant after 2 weeks of peri-implantitis, woven bones 
composed of newly formed trabeculae appeared, and imflammatory cells dispersed. The medullary cavity was irregular after collagen fibrils absorption, and there were 3-4 layers of osteoblasts in the bone lacunae, with active bone formation. These findings indicate that the Beagle model of pure titanium peri-implantitis under orthodontic force was successfully built in the experiment, and bone formation became active at 2 weeks after modeling.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering;, Models, Animal, Inflammation, Osteoblasts

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