中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (28): 4562-4566.doi: 10.3969/j.issn.2095-4344.2015.28.024

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

不同方式移植骨髓间充质干细胞在染矽尘大鼠体内的归巢

黄  明，周永梅，燕  玲，李  斌，吴奇峰，梁伟辉   

  1. 广东省职业病防治院，广东省广州市  510300
  • 出版日期:2015-07-02 发布日期:2015-07-02
  • 作者简介:黄明，女，1980年生，湖南省平江县人，汉族，2010年南方医科大学毕业，博士，副主任医师，主要从事职业病防治研究。
  • 基金资助:

    国家自然科学基金项目(81302396)；国家十二五科技支撑计划项目分课题(2014BAI12B01)；广东省职业病防治重点实验室(2012A061400007)；广东省医学科研基金(2015年)

In vivo homing of bone marrow mesenchymal stem cells transplanted through different ways in rats exposed to silica dust

Huang Ming, Zhou Yong-mei, Yan Ling, Li Bin, Wu Qi-feng, Liang Wei-hui   

  1. Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, Guangdong Province, China
  • Online:2015-07-02 Published:2015-07-02
  • About author:Huang Ming, M.D., Associate chief physician, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81302396; the National Science and Technology Support Program of China during the 12th Five-Year Period, No. 2014BAI12B01; the Project of Guangdong Provincial Key Laboratory of Occupational Disease Prevention, No. 2012A061400007; Guangdong Medical Research Foundation in 2015

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248

被引次数

1

摘要：

背景：骨髓间充质干细胞植入染矽尘大鼠体内能归巢到受损肺部，但何种植入途径在体内的归巢效果更好尚不清楚。
目的：动态比较观察不同途径移植骨髓间充质干细胞在染矽尘大鼠体内的分布情况。
方法：全骨髓贴壁法分离、培养供体大鼠骨髓间充质干细胞，用携带增强型绿色荧光蛋白基因的慢病毒(Lv-eGFP)转染骨髓间充质干细胞。受体大鼠用气管内注入法染尘，再随机分为经静脉组和经气管组，将转染Lv-eGFP的骨髓间充质干细胞分别经静脉、气管途径注入大鼠体内，在移植后的第1，2，3，4周处死大鼠，取心、肝、脾、肺、肾、脑组织进行冰冻切片，荧光显微镜下观察荧光，图文分析软件计算荧光强度。
结果与结论：两组大鼠肺组织均可见强烈、分布广泛且持续的绿色荧光，尤以气管、血管周围明显；两组荧光强度均随时间延长呈轻度减低趋势，但两组每周的荧光强度差异无显著性意义(P > 0.05)。两组大鼠其余各脏器早期也均可见荧光，其中肝、脾、心脏组织荧光强、分布广，肾、脑组织荧光相对较弱、分布较稀疏；随时间推移，各组织荧光均逐渐减弱，面积逐渐减少，到后期仅肝、脾组织可见到较弱、散在荧光分布，脑组织荧光几乎不可见。在同一时间点，两组除第1周脑组织荧光强度差异有显著性意义(P < 0.05)外，其余差异均无显著性意义(P > 0.05)。提示骨髓间充质干细胞经静脉和气管两种途径植入染矽尘大鼠体内归巢至受损肺部的效率相当。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells transplanted into rats exposed to silica dust can home to the injured lung, but the homing effects via different ways are still unclear.
OBJECTIVE: To comparatively observe the distribution of bone marrow mesenchymal stem cells transplanted via different ways into rats exposed to silica dust. 
METHODS: Bone marrow mesenchymal stem cells of donor rats were isolated through whole bone marrow adherent method and transfected by Lv-eGFP. Receptor rats were exposed to silica dust through windpipe injection and randomly divided into intravenous injection and intratracheal injection groups. Then, transfected bone marrow mesenchymal stem cells were injected via the vein and trachea into acceptor rats. The acceptor rats were killed at weeks 1, 2, 3, 4 after transplantation to take the lung, heart, liver, spleen, kidney, and brain tissue that were made into frozen sections and observed under fluorescence microscopy. The intensity of green 
fluorescence (absorbance value) was analyzed using image analysis software.
RESULTS AND CONCLUSION: Strong, wide and lasting green fluorescence was both observed in the lung tissue of intravenous injection and intratracheal injection groups, which was especially remarkable around the bronchus and blood vessels. The fluorescence intensities of both two groups were slightly decreased with time, but there was no difference between the two groups (P > 0.05). The fluorescence in the other organs of both two groups was also observed at early stage. It was stronger and wider in the liver, spleen and heart, while fainter and less in the kidney and brain, and reduced with time in all the organs. Fluorescence could be observed few and faint only in the liver and spleen at late stage, and could hardly be seen in the brain. The fluorescence intensities of the liver, spleen, heart, kidney and brain had no significant difference between the two groups at the same time (P > 0.05), but the fluorescence intensity in the brain at the 1st week showed significant difference between the two groups (P < 0.05). These findings indicate that the intravenous injection and intratracheal injection of bone marrow mesenchymal stem cells have similar homing effects in rats exposed to silica dust.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Lung, Drug Administration Routes

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