中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (24): 3798-3802.doi: 10.3969/j.issn.2095-4344.2015.24.006

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

软骨Ⅱ型胶原和蛋白多糖变化与温阳益髓方干预的影响

荆  琳1，郭志坤2，张洪美1，何名江1，单鹏程1   

  1. 1中国中医科学院望京医院骨关节一科，北京市  100102；
    2北京市羊坊店医院骨科，北京市  100038
  • 出版日期:2015-06-11 发布日期:2015-06-11
  • 通讯作者: 张洪美，主任医师，教授，中国中医科学院望京医院骨关节一科，北京市 100102
  • 作者简介:荆琳，男，1980年生，山东省平度市人，汉族，2007年江西中医药大学毕业，硕士，主治医师，主要从事关节外科、组织工程等方面研究。
  • 基金资助:

    北京市自然科学基金(7102166)

Effect of warming yang and benefiting marrow recipe on the expression of collagen type II and proteoglycans in the articular cartilage  

Jing Lin1, Guo Zhi-kun2, Zhang Hong-mei1, He Ming-jiang1, Shan Peng-cheng1   

  1. 1Department of Bone and Joint, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China; 
    2Department of Orthopedics, Yangfangdian Hospital of Beijing, Beijing 100038, China
  • Online:2015-06-11 Published:2015-06-11
  • Contact: Zhang Hong-mei, Chief physician, Professor, Department of Bone and Joint, Wangjing Hospi
  • About author:Jing Lin, Master, Attending physician, Department of Bone and Joint, Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China
  • Supported by:

    Beijing Natural Science Foundation, No. 7102166

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280

被引次数

2

摘要：

背景：骨关节炎发病过程中软骨破坏以Ⅱ型胶原和蛋白多糖破坏丢失最明显，而软骨基质中的主要成分Ⅱ型胶原和蛋白多糖的质和量的改变是导致骨关节软骨退变继而发生骨关节炎的直接原因。如何逆转或者阻止此进程的发展，成为当今医学的研究重点。
目的：观察温阳益髓方对兔膝骨关节炎软骨细胞Ⅱ型胶原和蛋白多糖表达的影响，探讨其保护软骨细胞的作用机制。
方法：选取9月龄新西兰大白兔96只，雌雄各半，采用伸直位石膏管型制动法进行骨关节炎造模，采用信封隐藏随机方法，分为4组：空白组(不做处理)、模型组(单纯造模)、中药组(每日灌胃“温阳益髓方”方药提取液)和西药组(每日灌胃“盐酸氨基葡萄糖”)， 中药组每日灌胃方药提取液24 mL/(kg•d)，西药组每日灌胃24 mL/(kg•d)，1次/d，持续6周。通过运用反转录聚合酶链技术，观察温阳益髓方对软骨Ⅱ型胶原和蛋白多糖的影响，并通过病理切片观察Ⅱ型胶原和蛋白多糖的染色结果。
结果与结论：空白组及中药组软骨表面平整，甲苯胺蓝染色染色均匀，未见明显失染，模型组及西药组软骨表面不规则，甲苯胺蓝染色染色极度不均，表、中层失染明显；模型组蛋白多糖及Ⅱ型胶原模型组显著低于空白组(P < 0.01)；中药组蛋白多糖及西药组蛋白多糖高于模型组(P < 0.05或P < 0.05)；中药组Ⅱ型胶原及西药组Ⅱ型胶原高于模型组(P < 0.05)；中药组蛋白多糖比西药组蛋白多糖高(P < 0.05)；中药组Ⅱ型胶原比西药组Ⅱ型胶原高(P < 0.05)。结果说明，温阳益髓方能增加兔膝骨关节炎软骨中Ⅱ型胶原表达及蛋白多糖的水平，对维持正常胶原表型、保护关节软骨有一定的作用。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 组织构建, 软骨组织工程, 温阳益髓, 骨关节炎, Ⅱ型胶原, 蛋白多糖, 北京市自然科学基金

Abstract:

BACKGROUND: Collagen type II and proteoglycan loss are two most obvious manifestations of cartilage damage in the onset of osteoarthritis. Changes in collagen type II and proteoglycan as the main components of cartilage matrix directly lead to cartilage degeneration and subsequently result in osteoarthritis. How to reverse or prevent the development of this process becomes the focus of medical research.
OBJECTIVE: To observe the effect of warming yang and benefiting marrow recipe on the expression of collagen type II and proteoglycan in the articular cartilage of knee osteoarthritis rabbits as well as to further explore the mechanism underlying chondrocyte protection.  
 METHODS: Ninety-six New Zealand rabbits, aged 9 months old, male and female, were selected to prepare osteoarthritis models in extension position using cast immobilization method, and were randomly divided into four groups: blank group (untreated), model group (simple modeling), Chinese medicine group (intragastric administration of extracts of warming yang and benefiting marrow recipe, 24 mL/kg/d) and western medicine group (intragastric administration of glucosamine hydrochloride, 24 mL/kg/d). Intragastric administration was done once a day for 6 weeks. RT-PCR technology was used to observe the effect of warming yang and benefiting marrow recipe on the expression of collagen type II and proteoglycans in the articular cartilage, and pathological examination was also done.
RESULTS AND CONCLUSION: The cartilage surface was smooth in the blank group and Chinese medicine group, with uniform toluidine blue staining, but in the model group and western medicine group, the cartilage surface was rough and the toluidine blue staining was extremely uneven with obvious loss of surface and middle layer dying. The expressions of cartilage proteoglycan and collagen type II in the model group were significantly lower than those in the blank group (P < 0.01) as well as in the Chinese medicine group and western medicine group (P < 0.05). In addition, the expressions of cartilage proteoglycan and collagen type II in the Chinese medicine group were higher than those in the western medicine group (P < 0.05). These findings indicate that the recipe of warming yang and benefiting marrow can enhance the expressions of collagen type II and proteoglycan, which can maintain the normal collagen phenotype and protect the articular cartilage.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

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