中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (5): 772-777.doi: 10.3969/j.issn.2095-4344.2015.05.020

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

不同性别肾缺血再灌注损伤模型小鼠MicroRNA的表达谱

唐伟伟，习小庆，胡红林，黄雅为，叶真逢，陈定义   

  1. 南昌大学第二附属医院泌尿外科，江西省南昌市  330006
  • 修回日期:2014-12-18 出版日期:2015-01-30 发布日期:2015-03-02
  • 通讯作者: 习小庆，硕士，主任医师，南昌大学第二附属医院泌尿外科，江西省南昌市 330006
  • 作者简介:唐伟伟，男，1989年生，湖南省永州市人，汉族，2012年南昌大学毕业，硕士，主要从事泌尿外科疾病的基础与临床研究。
  • 基金资助:

    江西省自然科学基金计划(20132BAB205009)

MicroRNA expression profiling in male and female model mice after renal ischemia-reperfusion injury

Tang Wei-wei, Xi Xiao-qing, Hu Hong-lin, Huang Ya-wei, Ye Zhen-feng, Chen Ding-yi   

  1. Department of Urology, Second Affiliated Hospital, Nanchang University, Nanchang 330006, Jiangxi Province, China
  • Revised:2014-12-18 Online:2015-01-30 Published:2015-03-02
  • Contact: Xi Xiao-qing, Master, Chief physician, Department of Urology, Second Affiliated Hospital, Nanchang University, Nanchang 330006, Jiangxi Province, China
  • About author:Tang Wei-wei, Master, Department of Urology, Second Affiliated Hospital, Nanchang University, Nanchang 330006, Jiangxi Province, China
  • Supported by:

    the Natural Science Foundation of Jiangxi Province, No. 20132BAB205009

PDF

702

被引次数

6

摘要：

背景：有研究表明肾缺血再灌注损伤存在性别差异，但其具体机制有待进一步研究。
目的：观察雌雄性小鼠缺血前后肾脏组织中MicroRNA表达及其差异，以期进一步研究MicroRNA在肾缺血再灌注损伤性别差异中的作用及机制。
方法：将雄性和雌性小鼠分别肾缺血45 min再灌注损伤24 h，同时设置雄性和雌性假手术组作对照。采用MicroRNA基因芯片技术检测雌雄小鼠肾缺血45 min再灌注损伤24 h及假手术后肾组织MicroRNA表达的差异，样品间表达差异的阈值为2倍。
结果与结论：在雌性肾缺血再灌注与雄性肾缺血再灌注组对比发现有表达上调的MicroRNA有5个；雌性肾缺血再灌注组与雌性假手术组对比发现有差异表达的MicroRNA有29个，其中上调的有MicroRNA有25个，下调的MicroRNA有4个；雄性肾缺血再灌注组与雄性假手术组对比发现有差异表达的MicroRNA有38个，其中上调的MicroRNA有9个，下调的MicroRNA有29个；雌性假手术组与雄性假手术组对比发现有差异表达的MicroRNA有102个，其中上调MicroRNA的有22个，下调的MicroRNA有80个。结果说明，不同性别小鼠肾缺血再灌注前后肾组织中微小核糖核酸表达存在差异，这些MicroRNA的差异表达可能导致不同性别小鼠肾脏对缺血再灌注损伤的敏感性及耐受性存在差异。



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

关键词: 实验动物, 泌尿系统损伤模型, 肾缺血再灌注损伤, 微小核糖核酸, BALB/C小鼠, 性别差异, 基因芯片, 聚类分析, 苏木精-伊红染色, 江西省自然科学基金

Abstract:

BACKGROUND: Renal ischemia-reperfusion injury has been shown to exhibit gender difference, but its precise mechanisms deserve further investigations.
OBJECTIVE: To investigate the differential expression of microRNAs in the kidney between female and male mice in order to study the effects and mechanisms of microRNA in pathogenesis of ischemia-reperfusion injury between different genders.
METHODS: Male and female mice received kidney ischemia for 45 minutes and reperfusion injury for 24 hours. Simultaneously, male and female sham surgery groups served as controls. The microRNA gene chip technology was used to detect the differences of microRNA expression in the kidney of male and female mice at 45 minutes after ischemia and 24 hours of reperfusion as well as after sham surgery. The threshold of difference in expression among samples was double.
RESULTS AND CONCLUSION: Five microRNAs were up-regulated between female and male ischemia-reperfusion injury groups. Twenty-nine microRNAs differentially expressed in the female ischemia-reperfusion group and female sham surgery group, including 25 up-regulated microRNAs and 4 down-regulated microRNAs. Thirty-eight microRNAs differentially expressed in male ischemia-reperfusion injury group and male sham surgery group, including 9 up-regulated microRNAs and 29 down-regulated microRNAs. 102 microRNAs differentially expressed in the female sham surgery group and male sham surgery group, including 22 up-regulated microRNAs and 80 down-regulated microRNAs. Results suggested that there was differential expression in microRNAs in the kidney before and after renal ischemia-reperfusion in male and female mice. These differentially expressed microRNAs may be lead to different sensitivity and tolerance to the ischemia-reperfusion injury in the kidney of male and female mice.



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

Key words: Tissue Engineering, Kidney, Reperfusion Injury, Microchip Analytical Procedures

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