中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (2): 192-195.doi: 10.3969/j.issn.2095-4344.2015.02.006

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

双醋瑞因干预体外培养关节软骨结缔组织生长因子的表达

王  声，廖俊琳，刘日光   

  1. 贵阳医学院附属医院骨科，贵州省贵阳市  550001
  • 收稿日期:2014-10-14 出版日期:2015-01-08 发布日期:2015-01-08
  • 通讯作者: 刘日光，博士，教授，主任医师，硕士生导师，贵阳医学院附属医院骨科，贵州省贵阳市 550001
  • 作者简介:王声，男，1985年生，河南省开封市人，汉族，硕士，主要从事骨关节病的基础研究。
  • 基金资助:

    贵州省科学技术基金资助项目(黔科合J字[2008]2286号)

Diacerein interferes the expression of connective tissue growth factor in the articular cartilage cultured in vitro

Wang Sheng, Liao Jun-lin, Liu Ri-guang   

  1. Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang 550001, Guizhou Province, China
  • Received:2014-10-14 Online:2015-01-08 Published:2015-01-08
  • Contact: Liu Ri-guang, M.D., Professor, Chief physician, Master’s supervisor, Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang 550001, Guizhou Province, China
  • About author:Wang Sheng, Master, Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang 550001, Guizhou Province, China
  • Supported by:

    Guizhou Provincial Science and Technology Foundation, No. QiankeheJ[2008]2286

PDF

456

被引次数

7

摘要：

背景：在骨关节炎软骨退变中结缔组织生长因子作为一种重要的效应分子在软骨细胞的增殖、分化方面发挥重要作用。临床应用双醋瑞因治疗骨关节炎已取得了良好的效果，但其治疗的确切机制尚不清楚。
目的：观察不同浓度双醋瑞因对体外白细胞介素1β诱导下软骨细胞中结缔组织生长因子的影响。
方法：体外培养SD大鼠关节软骨细胞，重组人白细胞介素1β刺激软骨细胞制备体外骨关节炎模型。实验分组：正常对照组不给予任何处理因素；模型对照组给予重组人白细胞介素1β；实验组给予不同浓度双醋瑞因+10 μg/L重组人白细胞介素1β。利用MTT比色法观察软骨细胞的增殖情况，Western Blot法检测结缔组织生长因子的表达。以上实验均重复3次。
结果与结论：MTT结果显示，与正常对照组比较，双醋瑞因能促进软骨细胞MTT增殖活性，以浓度为10-5 mol/L更明显(P < 0.01)，白细胞介素1β作用后各实验组软骨细胞增殖能力下降(P < 0.05)；但与正常对照组比较，无论是否加白细胞介素1β，浓度为10-4 mol/L和10-5 mol/L双醋瑞因仍能促进软骨细胞MTT增殖活性(P < 0.05)。Western Blot检测结果显示，白细胞介素1β能够降低结缔组织生长因子的表达(P < 0.01)，浓度为    10-5 mol/L双醋瑞因能够显著促进白细胞介素1β诱导下结缔组织生长因子的表达，显著高于模型对照组(P < 0.01)。提示双醋瑞因可以促进白细胞介素1β诱导下结缔组织生长因子的高表达，其可能是双醋瑞因促进软骨细胞的分化增殖，治疗骨关节炎的作用机制之一。

 


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 软骨组织工程, 双醋瑞因, 骨关节炎, 软骨细胞, 结缔组织生长因子, 白细胞介素1

Abstract:

BACKGROUND: In osteoarthritis cartilage degeneration, connective tissue growth factors as the important effector molecules play an important role in chondrocyte proliferation and differentiation. Clinical application of diacerein for the treatment of osteoarthritis has achieved good effect, but the exact mechanism of the treatment is unclear. 
OBJECTIVE: To observe the effect of different concentrations of diacerein on the expression of connective tissue growth factor in the articular cartilage induced with interleukin-1β in vitro.
METHODS: Chondrocytes of Sprague Dawley rats were cultured in vitro, and then the chondrocytes were induced with recombinant human interleukin-1β to prepare the osteoarthritis model. Rats in the normal control group received no treatment; rats in the model group were treated with recombinant human interleukin-1β; rats in the experimental group were treated with different concentrations of diacerein+10 μg/L recombinant human interleukin-1β. The proliferation of chondrocytes was observed with MTT assay, and Western blot was used to detect the expression of connective tissue growth factor. The experiments above were repeated for three times.    
RESULTS AND CONCLUSION: The MTT assay results showed that compared with the normal control group, the diacerein could promote the proliferation activity of chondrocytes, and more significant in the concentration of 10-5 mol/L (P < 0.01); the proliferation capacity of chondrocytes in the experimental group was decreased after induced with interleukin-1β (P < 0.05), but when compared with the normal control group, the diacerein with the concentrations of  10-4 mol/L and 10-5 mol/L could promote the MTT proliferation capacity of chondrocytes with or without interleukin-1β   (P < 0.05). Western blot results showed that interleukin-1β could reduce the expression of connective tissue growth factor (P < 0.01), and 10-5 mol/L diacerein could significantly promote the expression of connective tissue growth factor induced with interleukin-1β, and the effect was significantly higher than that in the model group (P < 0.01). Diacerein can promote expression of connective tissue growth factor induced with interleukin-1β, it may be one the mechanism of diacerein for promoting chondrocytes differentiation and proliferation and the treatment of osteoarthritis.

 


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: Osteoarthritis, Chondrocytes, Connective Tissue Growth Factor, Interleukin-1

中图分类号: