中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (52): 8409-8413.doi: 10.3969/j.issn.2095-4344.2014.52.009

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

医用新型Mg-Li-Ca合金材料体外生物相容性及生物活性评价

刘 睿1，姜 科2，赵保东3，曾荣昌4，徐 昊3，王兰英3   

  1. 1青岛大学口腔医学院，山东省青岛市 266003；2山东科技大学，山东省青岛市 266510；3青岛大学附属医院口腔科，山东省青岛市 266100；4山东科技大学材料科学与工程学院，山东省青岛市 266000
  • 修回日期:2014-11-12 出版日期:2014-12-17 发布日期:2014-12-17
  • 通讯作者: 赵保东，教授，青岛大学附属医院口腔科，山东省青岛市 266100 曾荣昌，教授，山东科技大学材料科学与工程学院，山东省青岛市 266000
  • 作者简介:刘睿，女，1988年生，山东省泰安市人，汉族，青岛大学口腔医学院在读硕士，主要从事口腔种植研究。

In vitro biocompatibility and bioactivity of a new medical Mg-Li-Ca alloy

Liu Rui1, Jiang Ke2, Zhao Bao-dong3, Zeng Rong-chang4, Xu Hao3, Wang Lan-ying3   

  1. 1College of Stomatology, Qingdao University, Qingdao 266003, Shandong Province, China; 2Shandong University of Science and Technology, Qingdao 266510, Shandong Province, China; 3Department of Stomatology, Affiliated Hospital of Qingdao University, Qingdao 266100, Shandong Province, China; 4College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266000, Shandong Province, China
  • Revised:2014-11-12 Online:2014-12-17 Published:2014-12-17
  • Contact: Zhao Bao-dong, Professor, Department of Stomatology, Affiliated Hospital of Qingdao University, Qingdao 266100, Shandong Province, China Zeng Rong-chang, Professor, College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266000, Shandong Province, China
  • About author:Liu Rui, Studying for master’s degree, College of Stomatology, Qingdao University, Qingdao 266003, Shandong Province, China

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597

被引次数

5

摘要：

背景：新型Mg-Li-Ca合金是否具有生物相容性和生物活性，目前还未被证实。

目的：通过体外实验评价新型医用Mg-Li-Ca合金的组织相容性及生物活性，初步探讨其作为医用植入材料的可行性。
方法：分别采用Mg-Li-Ca合金浸提液、纯Mg浸提液、AZ31B合金浸提液与α-MEM培养液培养第3代小鼠胚胎成骨细胞，培养1，3，5 d，采用MTT法检测细胞A值并计算相对增殖率；培养5，7 d，使用碱性磷酸酶试剂盒检测细胞碱性磷酸酶活性。将第3代小鼠胚胎成骨细胞与Mg-Li-Ca合金、纯Mg、AZ31B合金分别共培养于24孔板，培养1 d后扫描电镜观察材料表面黏附及增殖情况。
结果与结论：Mg-Li-Ca合金、纯Mg和AZ31合金对成骨细胞的细胞毒性为Ⅰ级，无细胞毒性，且Mg-Li-Ca合金对细胞毒性明显小于纯Mg和AZ31B合金，对成骨细胞生长增殖无显著影响，呈现出良好的生物相容性。Mg-Li-Ca合金和纯Mg对成骨细胞正常合成碱性磷酸酶无影响，具有良好的生物活性；AZ31B对成骨细胞正常合成碱性磷酸酶有显著影响。成骨细胞可在Mg-Li-Ca合金、纯Mg和AZ31合金表面正常黏附生长。表明新型Mg-Li-Ca合金有望成为新型骨科植入材料。

关键词: 生物材料, 材料相容性, 镁合金, Mg-Li-Ca合金, 生物相容性, 生物活性, 体外检测

Abstract:

BACKGROUND: Whether the new type of Mg-Li-Ca alloy has good biocompatibility and bioactivity or not has not been confirmed.

OBJECTIVE: To evaluate the in vitro biocompatibility and bioactivity of a new medical Mg-Li-Ca alloy, and to find the possibility of this Mg-Li-Ca alloy as a new type of implant material.
METHODS: The MC3T3-e1 (mouse osteoblasts) was used to be the test cells, then cultured and subcultured in vitro at passage 3. The cells were cultured and developed by leaching liquors of Mg-Li-Ca alloy, pure magnesium, and AZ31B alloy, and α-minimum essential medium, respectively. MTT assay was used to detect the absorbance values at days 1, 3, 5 of culture, and then the relative growth rate was calculated. Alkaline Phosphatase Colorimetric Assay Kit was used to check the activity of alkaline phosphatase at days 5 and 7 of culture. And the MC3T3-e1 cells were cocultured with Mg-Li-Ca alloy, pure magnesium, and AZ31B alloy, respectively; and at 1 day of coculture, cell adhesion and proliferation was observed under scanning electron microscopy.
RESULTS AND CONCLUSION: Mg-Li-Ca alloy, pure magnesium, and AZ31B alloy were non-cytotoxic to osteoblasts, and the cytotoxicity of Mg-Li-Ca alloy was lower than that of pure magnesium and AZ31B alloy, indicating Mg-Li-Ca alloy had no influence on osteoblast proliferation and had good biocompatibility. The alkaline phoaphatase activity was not influenced by Mg-Li-Ca alloy and pure magnesium that had good bioactivity. AZ31B had a significant impact on the normal synthesis of alkaline phosphatase in osteoblasts. The MC3T3-e1 cells can adhere and grow normally on the surface of Mg-Li-Ca alloy, pure magnesium, and AZ31B alloy. These findings indicate that the new type of mg-Li-Ca alloy is expected to be a new bone implant material.

Key words: materials testing, alloys, osteoblasts

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