中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (43): 6979-6984.doi: 10.3969/j.issn.2095-4344.2014.43.015

• 细胞外基质材料 extracellular matrix materials • 上一篇    下一篇

生物型抗菌盆底修复材料的制备与性能

凌  友1，2，徐  斌1，陈晓峰2   

  1. 1广东冠昊生物科技股份有限公司，再生型医用植入器械国家工程实验室，广东省广州市  510530；2华南理工大学材料科学与工程学院，广东省广州市  510641
  • 收稿日期:2014-08-06 出版日期:2014-10-15 发布日期:2014-10-15
  • 作者简介:凌友，男，1984年生，湖南省株洲市人，汉族，华南理工大学与广东冠昊生物科技股份有限公司博士后研究人员，主要从事生物医用材料和纳米缓释材料方面的研究。
  • 基金资助:

    国家科技部科技支撑计划项目(2012BAI17B00)；中央高校基金项目(2012ZP0001，2013ZB0005)；中国博士后基金项目(2013M531853)

Preparation and performance of biologic antimicrobial materials for pelvic tissue repair

Ling You1, 2, Xu Bin1, Chen Xiao-feng2   

  1. 1Grandhope Biotech Co., Ltd., National Engineering Laboratory for Regenerative Implantable Medical Devices, Guangzhou 510530, Guangdong Province, China; 2College of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong Province, China
  • Received:2014-08-06 Online:2014-10-15 Published:2014-10-15
  • About author:Ling You, M.D., Grandhope Biotech Co., Ltd., National Engineering Laboratory for Regenerative Implantable Medical Devices, Guangzhou 510530, Guangdong Province, China; College of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong Province, China
  • Supported by:

    the National Key Technology R&D Program of the Ministry of Science and Technology, No. 2012BAI17B00; the Fundamental Research Funds for Central Universities, No. 2012ZP0001, 2013ZB0005; China Postdoctoral Foundation, No. 2013M531853

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摘要：

背景：哺乳动物细胞外基质组成的生物型修复材料，在盆底组织修复及功能重建方面表现出较大潜力，但细菌感染对生物型修复材料的功能破坏是其中的一个较大问题。
目的：制备具备抗菌功能的生物型盆底修复材料。
方法：以壳聚糖为基质材料，通过静电吸附和自凝聚纳米粒制备技术获得包载有不同质量浓度替加环素(25，50，100 g/L)的壳聚糖大分子纳米粒混合液，再通过表面涂层技术将其涂覆于细胞外基质材料表面，制得抗菌细胞外基质材料。
结果与结论：红外光谱显示，抗菌细胞外基质材料出现壳聚糖的典型糖环骨架振动峰，并且在3 359 cm-1左右峰明显增宽，表明抗菌剂复合涂层修饰成功，由于替加环素的用量非常小，其红外图谱中未能显示其特征吸收峰。扫描电镜观察显示，抗菌细胞外基质材料保存了细胞外基质材料多层排列有序纤维丝构成的主体结构，并且在微丝表面和微丝之间还明显附着一层蓬松鳞片状涂层物质，材料微表面三维孔洞变小，但仍维持多孔连通特性。抑菌圈实验显示，抗菌细胞外基质材料对大肠杆菌和金黄色葡萄球菌具有良好的抗菌性能，并且随着抗菌剂浓度的增大，抑菌圈直径显著增加。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 纳米材料, 盆底修复材料, 抗菌性能, 纳米粒, 壳聚糖, 替加环素

Abstract:

BACKGROUND: The biological extracellular matrix materials become the focus of pelvic floor repair materials research because of its excellent biocompatibility and mechanical compatibility. However, bacterial infection can damage the function of biological repair materials.
OBJECTIVE: To prepare the biologic antimicrobial materials for pelvic tissue function repair.
METHODS: Chitosan and tigecycline-loaded chitosan nanoparticles suspension was prepared by the electrostatic adsorption and self-aggregation of nanoparticles preparation techniques. Then the suspension was coated onto the surface of extracellular matrix materials. The morphological examination was performed by scanning electron microscope. The antibacterial property was detected by solid plate microbial culture method.
RESULTS AND CONCLUSION: Infrared spectra displayed that aromatic ring skeleton vibration peak of chitosan occurred on the biologic antimicrobial materials, and it was significantly widened at about 3 359 cm-1, indicating the composite coating was successful to modify the surface of extracellular matrix materials. As a very small dose  
of tigecycline, there was no characteristic absorption peak on the infrared spectra. Scanning electron microscopy showed the porous structure of the material surface with some nanoparticles adhesion. The prepared materials had good antibacterial properties on Staphylococcus aureus and Escherichia coli, and the inhibition zone diameter was significantly increased with the increasing concentration of antibacterial agent. The biologic antimicrobial materials for pelvic tissue function repair were prepared successfully.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: nanoparticles, chitosan, pelvic floor

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