中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (38): 6099-6104.doi: 10.3969/j.issn.2095-4344.2014.38.006

• 骨组织构建 bone tissue construction • 上一篇    下一篇

富血小板血浆协同骨形态发生蛋白4促进骨愈合的效应

史剑杰，罗志宾，陈文雄   

  1. 广州医科大学附属第三医院口腔科，广东省广州市  510150
  • 收稿日期:2014-08-21 出版日期:2014-09-10 发布日期:2014-09-10
  • 通讯作者: 罗志宾，硕士，主治医师，广州医科大学附属第三医院口腔科，广东省广州市 510150
  • 作者简介:史剑杰，男，1965年生，湖南省益阳市人，汉族，1989年中山医科大学毕业，主任医师，主要从事口腔颌面外科研究。
  • 基金资助:

    广东省科技发展项目(2010B060900087)：课题名称：PRP协同BMP-4修复下颌骨缺损的动物实验研究

Bone morphogenetic protein-4 compounded with platelet-rich plasma promotes bone healing

Shi Jian-jie, Luo Zhi-bin, Chen Wen-xiong   

  1. Department of Stomatology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China
  • Received:2014-08-21 Online:2014-09-10 Published:2014-09-10
  • Contact: Luo Zhi-bin, Master, Attending physician, Department of Stomatology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China
  • About author:Shi Jian-jie, Chief physician, Department of Stomatology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China
  • Supported by:

    the Science and Technology Development Program of Guangdong Province, No. 2010B060900087

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摘要：

背景：研究表明骨形态发生蛋白在骨骼发育中的起到关键性作用；文献报道单独的富血小板血浆在动物或者临床试验中并不具备明显促进移植骨组织愈合的作用。
目的：验证富血小板血浆协同骨形态发生蛋白4促骨缺损区的成骨作用。
方法：24只新西兰大白兔构建上颌骨骨缺损模型，随机分为4组，每组6只。①空白对照组建模后不做特殊处理。②β-磷酸三钙+Bio-gide生物膜组为β-磷酸三钙0.1 g+Bio-gide生物膜1张。③富血小板血浆组为β-磷酸三钙0.1 g+Bio-gide生物膜1张+富血小板血浆1 mL。④富血小板血浆+骨形态发生蛋白4组为β-磷酸三钙0.1 g+Bio-gide生物膜1张+富血小板血浆1 mL+5 μg骨形态发生蛋白4。于造模后4，8，12周行大体观察、组织学观察及图像分析各组骨缺损区新生骨生成情况。
结果与结论：术后4周，富血小板血浆组、富血小板血浆+骨形态发生蛋白4组骨缺损区新生骨以及新生血管均多于β-磷酸三钙+Bio-gide组(P < 0.01)；β-磷酸三钙+Bio-gide组新成骨多于空白对照组(P < 0.01)。术后4，8，12周，富血小板血浆+骨形态发生蛋白4组骨缺损区新生骨均多于β-磷酸三钙+Bio-gide组、富血小板血浆组两组骨缺损区(P < 0.01)， β-磷酸三钙+Bio-gide组和富血小板血浆组均多于空白对照组(P < 0.01)。结果表明以β-磷酸三钙为支架，富血小板血浆复合骨形态发生蛋白可明显促进骨缺损的愈合。



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 骨组织工程, 骨形态发生蛋白, 富血小板血浆, 颌骨, β-磷酸三钙

Abstract:

BACKGROUND: Studies have shown that bone morphogenetic proteins play a key role in skeletal development. Platelet-rich plasma alone in animal or clinical trials cannot significantly promote bone graft healing.
OBJECTIVE: To investigate the osteogenesis effect of bone morphogenetic protein-4 compounded with platelet-rich plasma the bone defect area.
METHODS: Twenty-four New Zealand white rabbits were selected to establish maxillary bone defect models, and then were randomly divided into four groups, six rats in each group. Group A was blank control group; Group B was β-tricalcium phosphate (0.1 g)+Bio-gide group; group C was β-tricalcium phosphate (0.1 g)+Bio-gide+ platelet-rich plasma (1 mL) group; and group D wasβ-tricalcium phosphate (0.1 g)+Bio-gide+platelet-rich plasma (1 mL)+bone morphogenetic protein-4 (5 μg). Gross observation, histological observation and imaging analysis were performed for analysis of new bone formation at weeks 4, 8, 12 after modeling.
RESULTS AND CONCLUSION: After 4 weeks, group D had more new bone and vessels formed in the bone defect area than the group B (P < 0.01); however, the amount of new bone was higher in the group B than the group A (P < 0.01). After 4, 8, 12 weeks, the amount of new bone in the bone defect area was higher in the group D than the groups B and C (P < 0.01), and lowest in the group A (P < 0.01). In the β-tricalcium phosphate scaffold, platelet-rich plasma combined with bone morphogenetic protein can significantly promote the healing of bone defects.



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: maxilla, bone morphogenetic proteins, platelet-rich plasma, calcium phosphates

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