中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (37): 6682-6688.doi: 10.3969/j.issn.2095-4344.2013.37.023

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    下一篇

氟暴露患者外周血中MCM3的表达及肝肾功能变化

张亚楼1，赵  阳2，唐  莉3，吴起清1，白生宾1，钟近洁1   

  1. 1新疆医科大学基础医学院组织与胚胎学教研室，新疆维吾尔自治区乌鲁木齐市  830011；新疆医科大学第一附属医院，2烧伤整形科，3传染病中心，新疆维吾尔自治区乌鲁木齐市  830011
  • 收稿日期:2013-03-07 修回日期:2013-04-28 出版日期:2013-09-10 发布日期:2013-09-10
  • 通讯作者: 钟近洁，教授，新疆医科大学基础医学院组织与胚胎学教研室，新疆维吾尔自治区乌鲁木齐市 830011 zhongjinjie@gmail.com
  • 作者简介:张亚楼☆，男，1976年生，重庆市人，汉族，2010年新疆医科大学毕业，博士，副教授，主要从事分子生物学诊断技术研究。 zhangyalou12@yahoo.com.cn 并列第一作者：赵阳，男，1974年生，新疆维吾尔自治区哈密市人，汉族，南方医科大学在读博士，副主任医师，主要从事细胞信号转导异常与疾病研究。 gorgezy@163.com
  • 基金资助:

    国家自然科学基金赞助(30860249, 81102084)*

Expression of minichromosome maintenance 3 from the peripheral blood of fluorosis patients and the liver and renal function

Zhang Ya-lou1, Zhao Yang2, Tang Li3, Wu Qi-qing1, Bai Sheng-bin1, Zhong Jin-jie1   

  1. 1Department of Histology and Embryology, Basic Medical Institute, Xinjiang Medical University, Urumqi  830011, Xinjiang Uygur Autonomous Region, China; 2Department of Burns and Plastic Surgery, 3Infectious Disease Centre, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830011, Xinjiang Uygur Autonomous Region, China
  • Received:2013-03-07 Revised:2013-04-28 Online:2013-09-10 Published:2013-09-10
  • Contact: Zhong Jin-jie, Professor, Department of Histology and Embryology, Basic Medical Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China zhongjinjie@gmail.com
  • About author:Zhang Ya-lou☆, M.D., Associate professor, Department of Histology and Embryology, Basic Medical Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China zhangyalou12@yahoo.com.cn Zhao Yang☆, Studying for doctorate, Associate chief physician, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China gorgezy@163.com Zhang Ya-lou and Zhao Yang contributed equally to this study.
  • Supported by:

    National Natural Science Foundation of China, No. 30860249*, 81102084*

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摘要：

背景：前期研究发现MCM3与氟中毒相关，但其在氟中毒早期暴露者中的表达情况尚不清楚。
目的：分析氟暴露患者及对照人群外周血中MCM3 mRNA的表达量。
方法：选取饮水型氟中毒轻度患者(暴露组)、对照人群(非暴露组)各11例，采用SYBRGreen1嵌合荧光法实时定量PCR检测外周血单个核细胞中MCM3 mRNA的表达，同时测定两组肝肾功能指标。
结果与结论：暴露组及非暴露组MCM3 mRNA表达量分别为0.573 60±0.102 59、0.550 0±0.171 81，两组差异无显著性意义(P > 0.05)。所测的肝肾功能指标在两组中差异无显著性意义。结果显示轻度氟暴露对患者外周血单个核细胞中MCM3 mRNA表达无显著影响。氟对暴露者肝、肾功能的影响需要选取更多的指标综合分析。

关键词: 组织构建, 组织构建临床实践, 氟, MCM3基因, 定量反转录聚合酶链反应, 肝功能, 肾功能, 国家自然科学基金

Abstract:

BACKGROUND: Previous studies have shown that minichromosome maintenance 3 is related with fluorosis, but the expression of minichromosome maintenance 3 in fluorosis patients is not clear yet.
OBJECTIVE: To analyze the mRNA expression level of minichromosome maintenance 3 in peripheral blood from patients exposed to fluoride and normal controls.
METHODS: Eleven patients with mild fluorosis by drinking water (exposure group) and 11 cases of control (non-exposure group) were selected for research. SYBRGreen1 real-time quantitative PCR was used to determine the mRNA expression of minichromosome maintenance 3 in peripheral blood mononuclear cells, and the liver and renal function indicators were detected.
RESULTS AND CONCLUSION: mRNA expressions of minichromosome maintenance 3 in the exposure group and non-exposure group were (0.573 60±0.102 59) and (0.550 0±0.171 81), respectively, and there was no significant difference between two groups (P > 0.05). There were no significant differences in the liver and renal function indicators between two groups. The results indicate that mild fluorosis has no significant effect on mRNA expression of minichromosome maintenance 3 in the peripheral blood mononuclear cells. More indicators are needed to compressively analyze the effect of fluoride on the liver and renal functions.

Key words: fluorine, genes, endemic diseases, reverse transcriptase polymerase chain reaction

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