中国组织工程研究

中国组织工程研究

• 神经组织构建 nerve tissue construction • 上一篇    下一篇

双向脉冲可实现的神经纤维选择性兴奋

朱晓瑾1，王  辉2，张  旭1，任朝晖1，刘庆凯1，李春婵1，阎立丽1   

  1. 1首都医科大学生物医学工程学院，北京市  100069；2首都医科大学附属北京朝阳医院耳鼻咽喉头颈外科，首都医科大学耳鼻喉科学院，首都医科大学耳鼻咽喉头颈科学教育部重点实验室，北京市    100020
  • 收稿日期:2013-01-11 修回日期:2013-02-28 出版日期:2013-08-13 发布日期:2013-08-13
  • 通讯作者: 张旭，博士，教授，首都医科大学生物医学工程学院，北京市 100069 zhangxu@ccmu.edu.cn
  • 作者简介:朱晓瑾★，女，1988年生，江苏省淮安市人，汉族，首都医科大学在读硕士。xiao_jin_love@163.com
  • 基金资助:

    国家自然科学基金(30872860)；北京自然科学基金(3122011)。

Selective nerve excitability induced by symmetric biphasic pulses

Zhu Xiao-jin1, Wang Hui2, Zhang Xu1, Ren Zhao-hui1, Liu Qing-kai1, Li Chun-chan1, Yan Li-li1   

  1. 1School of Biomedical Engineering, Capital Medical University, Beijing  100069, China; 2Department of Otolaryngology, Beijing Chao-Yang Hospital of Capital Medical University, Capital University of Medical Sciences School of Otolaryngology, Key Laboratory of Otolaryngology Head and Neck Surgery (Capital Medical University), Ministry of Education, Beijing  100020, China
  • Received:2013-01-11 Revised:2013-02-28 Online:2013-08-13 Published:2013-08-13
  • Contact: Zhang Xu, M.D., Professor, School of Biomedical Engineering, Capital Medical University, Beijing 100069, China zhangxu@ccmu.edu.cn
  • About author:Zhu Xiao-jin★, Studying for master’s degree, School of Biomedical Engineering, Capital Medical University, Beijing 100069, China xiao_jin_love@163.com
  • Supported by:

    National Natural Science Foundation of China, No. 30872860*; Beijing Natural Science Foundation, No. 3122011*

PDF

499

被引次数

2

摘要：

背景：研究证明利用电刺激外周神经纤维可恢复一些因失去中枢神经控制的肌肉的功能。
目的：验证双电极1 mm较近距离下双向方波脉冲实现神经选择性兴奋的正确性，并基于此实现神经的选择性兴奋。
方法：成年Wistar大鼠8只，麻醉后暴露大鼠坐骨神经，将电极小心放于坐骨神经干，建立神经选择性刺激模型。实验用电极为自制Cuff双极性电极，刺激器采用的是Grass S88刺激器和AWG2005任意波形信号发生器。采取双电极双向刺激方式，两个电极之间距离为1 mm，刺激波形选用脉宽为0.2 ms的对称双向脉冲，其输出脉冲的幅度、脉宽和延时均可调。调节刺激强度，研究双电极双向刺激下神经兴奋性的规律，以此实现神经的选择性兴奋，并利用“碰撞法”原理验证利用双电极双向刺激方法实现神经选择性兴奋的可行性。
结果与结论：实验过程中神经动作电位的变化将经P511放大器放大后接入示波器显示，双电极刺激波形为脉宽为0.2 ms的对称双向脉冲。随着刺激幅度的增大，实现神经的选择性兴奋。说明用距离很近(1 mm)的双电极双向对称脉冲的方法实现了神经的选择性兴奋，并利用“碰撞法”原理证实了此种方法的有效性和可行性。

关键词: 组织构建, 神经组织构建, 选择性兴奋, 神经, 双向脉冲, 双电极刺激, 动作电位, 动物实验, 国家自然科学基金

Abstract:

BACKGROUND: Studies have shown that the application of electrical stimulation of peripheral nerve fibers can recover partial muscle functions due to the loss of central nervous control.
OBJECTIVE: To verify the feasibility of selective nerve excitatory effectively used the symmetric biphasic pulses under the bipolar electrodes stimulation of 1 mm.
METHODS: Eight adult Wistar rats were selected to expose the sciatic nerves after anesthesia and then the electrodes were placed on the sciatic nerves carefully to establish the model of selective nerve stimulation. Experimental electrode was homemade Cuff bipolar electrode, and the electrode stimulators were Grass S88 stimulator and AWG2005 arbitrary waveform signal generator. The two-way dual-electrode stimulation was used. The distance between two electrodes was 1 mm, and the stimulation waveform was symmetric biphasic pulse with the width of 0.2 ms. The output pulse amplitude, pulse width and delay could be adjusted. The stimulation intensity was adjusted, and the law of nerve excitability was detected under two-way dual-electrode stimulation, in order to achieve selective nerve excitability, and the feasibility of two-way dual-electrode stimulation to achieve selective nerve excitatory was verified with “collision” method.
RESULTS AND CONCLUSION: The change of nerve action potential was amplified by P511 amplifier and then  linked into oscilloscope for displaying, and dual-electrode stimulation waveform was the symmetric biphasic pulse with the width of 0.2 ms. With the increasing of stimulus amplitude, we achieved the selective nerve excitatory. The results indicate the selective nerve excitatory can be achieved with the closed (1 mm) dual-electrode symmetric pulse, and the feasibility and effectiveness of this method can be verified with “collision” principle.

Key words: tissue construction, nerve tissue construction, selective nerve excitatory, nerve, bipolar pulse, dual-electrode stimulation, action potential, animal experiments, National Natural Science Foundation of China

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