中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (25): 4570-4576.doi: 10.3969/j.issn.2095-4344.2013.25.002

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

微弧氧化/电化学沉积钙磷涂层纯钛种植体的骨内植入

马  盈1，孟祥才2，王  静2，李德超1   

  1. 1佳木斯大学口腔医院，黑龙江省佳木斯市  154002
    2佳木斯大学，黑龙江省佳木斯市  154007
  • 收稿日期:2012-12-19 修回日期:2013-01-23 出版日期:2013-06-18 发布日期:2013-06-18
  • 通讯作者: 李德超，博士，副教授，佳木斯大学口腔医院口腔种植科，黑龙江省佳木斯市 154002 dechaoli2004@yahoo.com.cn
  • 作者简介:马盈★，女，1987年生，黑龙江省巴彦县人，汉族，佳木斯大学在读硕士，主要从事口腔种植方面的研究。 maying6666@yeah.net
  • 基金资助:

    佳木斯大学重点项目(SZ2009-001)。

Endosseous implantation of calcium phosphate coated titanium implant prepared via micro-arc oxidation/electrochemical deposition  

Ma Ying1, Meng Xiang-cai2, Wang Jing2, Li De-chao1   

  1. 1 Stomatology Hospital, Jiamusi University, Jiamusi  154002, Heilongjiang Province, China
    2 Jiamusi University, Jiamusi  154007, Heilongjiang Province, China
  • Received:2012-12-19 Revised:2013-01-23 Online:2013-06-18 Published:2013-06-18
  • Contact: Li De-chao, M.D., Associate professor, Stomatology Hospital, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China dechaoli2004@yahoo.com.cn
  • About author:Ma Ying★, Studying for master’s degree, Stomatology Hospital, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China maying6666@yeah.net
  • Supported by:

    the Key Project of Jiamusi University, No. SZ 2009-001

PDF

507

被引次数

18

摘要：

背景：近年来已有对微弧氧化/电化学沉积技术制备涂层在材料性能方面的相关报道，但对这种材料植入体内的性能研究较少见。
目的：观察纯钛种植体经微弧氧化/电化学沉积处理后的骨结合和新骨形成情况。
方法：通过微弧氧化/电化学沉积方法在纯钛上制备含钙磷元素的涂层，然后将该种植体和纯钛种植体分别植入羊两侧胫骨种植窝内，于动物处死前15，5 d分别进行注射四环素进行四环素标记。术后4，12周分别进行X射线、扫描电镜及激光共聚焦观察。
结果与结论：两侧X射线表现相似，种植体周围均无明显阴影，骨小梁排列和骨质密度与宿主骨基本一致。术后4周时，在电镜下可观察到两组种植体和骨组织之间均有间隙，部分见骨性结合；术后12周时，微弧氧化/电化学沉积种植体组可形成新骨，并且新骨与种植体和原来骨组织结合紧密，涂层与钛基体没有明显间隙，纯钛种植体组也可见新骨生成，但可看到明显裂隙。激光共聚焦观察显示，微弧氧化/电化学沉积种植体组双标记带间距离及骨矿化沉积率均高于纯钛种植体组(P < 0.05)。表明微弧氧化/电化学沉积处理可增强纯钛种植体的骨结合能力及新骨形成。

关键词: 生物材料, 组织工程骨材料, 微弧氧化, 纯钛种植体, 电化学沉积, 涂层, 双标记, 骨结合, 其他基金

Abstract:

BACKGROUND: There are studies concerning material properties of coating prepared by micro-arc oxidation and electrochemical deposition, but there are few studies addressing properties of this kind of material implanted in the body.
OBJECTIVE: To observe the synostosis and new bone formation of the pure titanium implant prepared by micro-arc oxidation/electrochemical deposition.
METHODS: This research produces calcium and phosphate coatings on pure titanium though micro-arc oxidation/Electrochemical deposition technology, and the sheep were implanted with micro arc-oxidation/electrochemical deposition implant and pure titanium implant on both sides of the tibia planting socket. All the animals were injected with tetracycline at 15 and 5 days before sacrificing. Radiograph, scanning electron microscope and the laser scanning confocal microscope were used at 4 and 12 weeks post-implantation.
RESULTS AND CONCLUSION: The radiograph shows that there was no shade produced around the two kinds of different materials. The trabeculae arrangement and the density of bone were almost the same as in the host bone. There was interspace between two groups of implant and bone tissue under the scanning electron microscope at 4 weeks post-implantation, and synostosis existed. At 12 weeks post-implantation, the implant prepared by micro-arc oxidation/electrochemical deposition promoted the formation of new bone. Moreover, new bone and implant combined with the original bone tissue closely and there was no obvious gap between the coating and the titanium matrix. The new bone formation was visible in the pure titanium implant group at 12 weeks post-implantation, but the obvious crack was observed. The laser scanning confocal microscopy revealed that the double marking the distance between the belts and the bone mineralization deposition rate in the implant prepared by micro-arc oxidation/electrochemical deposition were higher than that in the pure titanium implant group (P < 0.05). These results indicated that micro-arc oxidation/electrochemical deposition can enhance the synostosis ability and promote the new bone formation of pure titanium implant.

Key words: biomaterials, tissue-engineered bone materials, micro-arc oxidation, pure titanium implant, electrochemical deposition, coating, double labeling, synosteosis, other grants-suported paper

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