中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8636-8640.doi: 10.3969/j.issn.2095-4344.2012.46.017

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

转化生长因子β2对人晶状体上皮细胞E-钙黏素和α-平滑肌肌动蛋白表达的影响

朱 艳，朱玉广，钟莹莹，杜孝楠，张 荣，王 杰   

  1. 山东省潍坊医学院附属医院眼科中心，山东省潍坊市 261031
  • 收稿日期:2012-03-08 修回日期:2012-04-24 出版日期:2012-11-11 发布日期:2012-11-11
  • 通讯作者: 朱玉广，博士，副教授，山东省潍坊医学院附属医院眼科中心，山东省潍坊市 261031 yg_zhu.md@tom.com
  • 作者简介:朱艳★，女，1975年生，湖北省丹江口市人，汉族，2004年华中科技大学同济医学院毕业，硕士，讲师，主要从事青光眼、白内障方面的研究。 azhu1975@sina.com

Transforming growth factor-beta 2 effects on the expression of E-cadherin and alpha-smooth muscle actin in human lens epithelial cells

Zhu Yan, Zhu Yu-guang, Zhong Ying-ying, Du Xiao-nan, Zhang Rong, Wang Jie   

  1. Eye Center, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong Province, China
  • Received:2012-03-08 Revised:2012-04-24 Online:2012-11-11 Published:2012-11-11
  • Contact: Zhu Yu-guang, Doctor, Associate professor, Eye Center, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong Province, China yg_zhu.md@tom.com
  • About author:Zhu Yan★, Master, Lecturer, Eye Center, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong Province, China azhu1975@sina.com

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摘要：

背景：有研究表明，人晶状体上皮细胞的间质转分化是后发性白内障的主要病理改变，转化生长因子β2可以诱导间质转分化的发生。
目的：体外培养人晶状体上皮细胞，观察转化生长因子β2对人晶状体上皮细胞转分化相关蛋白E-钙黏蛋白和α-平滑肌肌动蛋白表达的影响。
方法：利用组织块法体外培养人晶状体上皮细胞并传代，选择传5代的细胞进行实验，采用100 ng/L转化生长因子β2诱导48 h，反转录-聚合酶链反应方法检测E-钙黏蛋白、α-平滑肌肌动蛋白mRNA的表达，应用蛋白质印迹法Western blot检测其蛋白表达。
结果与结论：转化生长因子β2处理人晶状体上皮细胞48 h后，细胞E-钙黏蛋白表达明显减弱，α-平滑肌肌动蛋白的表达明显增强。提示转化生长因子β2可以成功诱导晶状体上皮细胞间质转分化，转化生长因子β2处理的晶状体上皮细胞可以作为间质转分化的细胞模型。

关键词: 晶状体上皮细胞, 转化生长因子β2, E-钙黏蛋白, α-平滑肌肌动蛋白, 转分化

Abstract:

BACKGROUND: Studies have shown that mesenchymal transition in human lens epithelial cells is the main pathological change after cataract. Transforming growth factor-β2 can induce the mesenchymal transition.
OBJECTIVE: To in vitro culture human lens epithelial cells and to investigate the effect of transforming growth factor-β2 on the expression of E-cadherin and α-smooth muscle actin in human lens epithelial cells.
METHODS: First, human lens epithelial cells were cultured in vitro by tissue block method and passaged. Then, the 5th generation of human lens epithelial cells were collected and treated with transforming growth factor-β2 (100 ng/L) for inducement for 48 hours. Next, the expression of α-smooth muscle actin mRNA and E-cadherin mRNA was detected by reverse transcription-PCR, and the expression of the two proteins mentioned above were detected with Western blot method.
RESULTS AND CONCLUSION: After human lens epithelial cells were treated with transforming growth factor-β2 for 48 hours, the expression of E-cadherin was decreased significantly; while the expression of α-smooth muscle actin was increased significantly. These results suggest that transforming growth factor-β2 can successfully induce mesenchymal transition in human lens epithelial cells, and the lens epithelial cells treated with transforming growth factor-β2 can be used as a cell model for mesenchymal transition.

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