中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8607-8610.doi: 10.3969/j.issn.2095-4344.2012.46.011

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

体外阻断基质细胞衍生因子1/CXC趋化因子受体4信号通路后人关节软骨组织Ⅱ型胶原和聚集蛋白聚糖的表达

李彦林，王国梁，曹 斌，何 川，高 岗，陈文栋，马 珂，许 鹏，杨 光   

  1. 昆明医学院第一附属医院运动医学科，云南省昆明市 650031
  • 收稿日期:2012-02-17 修回日期:2012-03-27 出版日期:2012-11-11 发布日期:2012-11-11
  • 作者简介:李彦林☆，男，1969年生，云南省马龙县人，汉族，2000年华西医科大学毕业，博士，教授，博士生导师，主要从事骨关节炎防治的研究。 yanlinli1969@yahoo.com.cn

Expression of type Ⅱ collagen and aggrecan in human articular cartilage tissues after blocking stromal cell derived factor-1/chemokine receptor 4 signal pathway in vitro

Li Yan-lin, Wang Guo-liang, Cao Bin, He Chuan, Gao Gang, Chen Wen-dong, Ma Ke, Xu Peng, Yang Guang   

  1. Department of Sport Medicine, First Affiliated Hospital of Kunming Medicine College, Kunming 650031, Yunnan Province, China
  • Received:2012-02-17 Revised:2012-03-27 Online:2012-11-11 Published:2012-11-11
  • About author:Li Yan-lin☆, Doctor, Professor, Doctoral supervisor, Department of Sport Medicine, First Affiliated Hospital of Kunming Medicine College, Kunming 650031, Yunnan Province, China yanlinli1969@yahoo.com.cn

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395

被引次数

11

摘要：

背景：基质细胞衍生因子1/CXC趋化因子受体4信号通路在骨关节炎的发病中起关键作用。
目的：探讨AMD3100体外阻断基质细胞衍生因子1/CXC趋化因子受体4信号通路后对培养的关节软骨组织Ⅱ型胶原、聚集蛋白聚糖mRNA表达及Ⅱ型胶原蛋白表达的影响。
方法：将骨关节炎软骨和创伤性截肢患者正常软骨分别分为3个小组即实验组，实验对照组，空白对照组。将其置于含基质细胞衍生因子1和AMD3100，含基质细胞衍生因子1和MAB310，只含基质细胞衍生因子1的培养液培养。
结果与结论：实验组软骨组织内的Ⅱ型胶原和聚集蛋白聚糖mRNA表达量、Ⅱ型胶原蛋白含量高于实验对照组和空白对照组(P < 0.05)。可见基质细胞衍生因子1通过基质细胞衍生因子1/CXC趋化因子受体4信号通路诱导人关节软骨中Ⅱ型胶原和聚集蛋白聚糖的降解；AMD3100可阻断该信号通路及减少软骨中Ⅱ型胶原和聚集蛋白聚糖的降解，延缓关节软骨组织的退变；AMD3100不能使已退变的骨关节炎软骨内的Ⅱ型胶原和聚集蛋白聚糖含量恢复到正常水平。

关键词: 骨性关节炎, 基质细胞衍生因子1/CXC趋化因子受体4信号通路, AMD3100, Ⅱ型胶原, 聚集蛋白聚糖

Abstract:

BACKGROUND: Stromal cell derived factor-1(SDF-1)/chemokine receptor 4 (CXCR4) signal pathway plays a key role in the pathogenesis of osteoarthritis.
OBJECTIVE: To explore the effect of SDF-1/CXCR4 signal pathway on the expression of type Ⅱ collagen and aggrecan mRNA of the synthesis in human articular cartilage after blocking the SDF-1/CXCR4 signal pathway with AMD3100.
METHODS: The cartilage blocks from osteoarthritis patients who had total knee replacement and normal cartilage blocks from patients who had traumatic amputation were collected and divided into three groups: experimental group, experimental control group and blank control group. The three groups were cultured in the nutrient solution containing of SDF-1 and AMD3100, SDF-1 and MAB310, as well as SDF-1 only, respectively.
RESULTS AND CONCLUSION: The expressions of type Ⅱ collagen and aggrecan mRNA, as well as type Ⅱ collagen content in the experimental group was higher than those in the experimental control group and blank control group (P < 0.05). These results suggest that SDF-1 can induce the degradation of type Ⅱ collagen and aggrecan thruogh the SDF-1/CXCR4 signaling pathway. Besides, AMD3100 can block SDF-1/CXCR4 signal pathway and reduce the degradation of type II collagen and aggrecan, and therefore slow down the degeneration of articular cartilage. But AMD3100 cannot recover type II collagen and aggrecan in osteoarthritis cartilage to normal levels

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