中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7883-7887.doi: 10.3969/j.issn.2095-4344.2012.42.018

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

小鼠角膜接触镜电极的制备和评价

郑钦象1，朱佳丽1，潘妙珍1，石 赢1, 2，李文生1, 3○   

  1. 1温州医学院附属眼视光医院，浙江省温州市 325027
    2温州医学院RGP研发中心，浙江省温州市 325027
    3美国马萨诸塞州立大学眼科，美国，马萨诸塞州 伍斯特01545
  • 收稿日期:2012-02-08 修回日期:2012-03-19 出版日期:2012-10-14 发布日期:2012-10-14
  • 通讯作者: 李文生，博士，教授，博士生导师，温州医学院附属眼视光医院，浙江省温州市 325027；美国马萨诸塞州立大学眼科，美国，马萨诸塞州 伍斯特01545 drlws@163.net
  • 作者简介:郑钦象☆，男，1982年生，浙江省温州市人，2011年温州医学院毕业，博士，讲师，主要从事眼科学相关研究。 zhengqinxiang@yahoo.com.cn

Preparation and evaluation of contact lens electrode in mice

Zheng Qin-xiang1, Zhu Jia-li1, Pan Miao-zhen1, Shi Ying1, 2, Li Wen-sheng1, 3   

  1. 1Optometry & Ophthalmology Hospital Affiliated to Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China
    2RGP Research and Development Centre, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China
    3Department of Ophthalmology, University of Massachusetts Medical School, Worcester 01545, MA, USA
  • Received:2012-02-08 Revised:2012-03-19 Online:2012-10-14 Published:2012-10-14
  • Contact: Li Wen-sheng, Professor, Doctoral supervisor, Optometry & Ophthalmology Hospital Affiliated to Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China; Department of Ophthalmology, University of Massachusetts Medical School, Worcester 01545, MA, USA Worcester, MA, USA drlws@163.net
  • About author:Zheng Qin-xiang☆, Doctor, Lecturer, Optometry & Ophthalmology Hospital Affiliated to Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China zhengqinxiang@yahoo.com.cn

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摘要：

背景：小鼠是常用作研究人类视网膜疾病发生、发展规律和防治措施的动物模型，视觉电生理检查已经成为检测小鼠视网膜功能的常规客观检查手段之一。
目的：研制一种新型的小鼠角膜接触镜电极，并检测其在视网膜电图检查过程中的有效性和重复性。
方法：首先测量不同年龄的C57BL6/J小鼠(共24只)的角膜曲率半径和角膜直径；根据所测量得到的小鼠角膜生物参数制备针对不同年龄段小鼠角膜接触镜；由数控系统车床加工获取角膜接触镜；将电极与角膜接触镜相黏附；另选取12只6周龄C57BL6/J小鼠平均分配到2组分别使用传统环状电极和角膜接触镜电极进行视网膜电图检测，分别记录暗适应和明适应视网膜电图的b波振幅，间隔1周测量1次，共3次；2周裂隙灯显微镜小鼠角膜形态。
结果与结论：小鼠角膜直径在不同年龄的小鼠中具有较大差异，在2.23-3.41 mm；设计小鼠角膜接触镜的曲率为2.00 mm，直径为3.5 mm；小鼠角膜接触镜电极在测量暗适应和明适应b波振幅平均值为传统环状电极的82.7%和80.3%，而反复多次检测时，角膜接触电极稳定性高于传统环状电极；使用角膜接触镜电极检测小鼠角膜新生血管翳明显少于环形电极。表明小鼠角膜接触镜电极可以有效的应用于视觉电生理的检测，虽其测量b波振幅较传统环状电极低，但其在多次测量的稳定性较高，并且在电生理检测过程中可以有效降低小鼠角膜损伤。

关键词: 角膜接触镜电极, 小鼠, 视觉电生理, 角膜直径, b波振幅

Abstract:

BACKGROUND: Mice are the animal model used most commonly for studying the occurrence, development and prevention of human retinal diseases. Retinal electrophysiological tests have become one of the routine methods for the test of mouse retina function.
OBJECTIVE: To develop a new kind of contact lens electrode for mouse corneal electroretinogram (ERG) recordings, and to detect its effectiveness and repeatability in the process of the test.
METHODS: First, the cornea radius of curvature and the cornea diameter of 24 C57BL6/J mice at various ages were measured. Contact lens in mice of different ages were prepared according to the measured biological parameters of mouse cornea and processed by lathe digital controlled system. The electrode was then adhered to the contact lens and became a new kind of ERG electrode. Another 12 C57BL6/J mice aged 6 weeks were averagely divided into two groups and performed ERG test with traditional annular metallic electrode and this new designed contact lens electrode, respectively. B-wave amplitudes of both dark-adapted and light-adapted ERG in the two groups were recorded every two weeks, totally three times. Cornea status of mice was evaluated by slit lamp microscope after 2 weeks.
RESULTS AND CONCLUSION: There was significant difference in cornea diameter of different age mice (1.39- 1.48 mm). The designed radius of curvature of the cornea was 2.00 mm and corneal diameter was 3.5 mm. The mean values of B-wave amplitudes of both dark-adapted and light-adapted ERG using the contact lens electrode were recorded 82.7% and 80.3% of those tested by the traditional annular electrode. However the stability of contact lens electrode was much higher than that of the traditional annular electrode after repeated testing. ERG test by using contact lens electrode showed that mouse corneal neovascularization in the contact lens electrode group was obviously less than that in the traditional electrode group. These results suggest that mouse contact lens electrode can be efficiently applied in ERG test. Although its b-wave amplitude was lower than that of traditional annular electrode, its stability in repeated test was higher, and it could effectively decrease mouse corneal injuries.

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