中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (40): 7508-7513.doi: 10.3969/j.issn.2095-4344.2012.40.019

• 移植与中医药 transplantation and traditional Chinese medicine • 上一篇    下一篇

单侧输尿管梗阻肾间质纤维化模型大鼠与川芎嗪的干预

李健芝1，庾江东2，梁 瑜3，刘玉明2，胡 丽1，杨 波4，周秀田3   

  1. 1南华大学护理学院，湖南省衡阳市 421001
    2衡阳市中心医院，湖南省衡阳市 421008
    3南华大学医学院，湖南省衡阳市 421001
    4南华大学附属第一医院，湖南省衡阳市 421001
  • 收稿日期:2012-03-01 修回日期:2012-03-23 出版日期:2012-09-30 发布日期:2012-09-30
  • 作者简介:李健芝★，女，1972年生，湖南省衡阳市人，2004年中南大学毕业，硕士，副教授，从事内科护理教学及科研工作。 ljz111@163.com

Effects of tetramethylpyrazine in rats with unilateral ureteral obstruction renal fibrosis

Li Jian-zhi1, Yu Jiang-dong2, Liang Yu3, Liu Yu-ming2, Hu Li1, Yang Bo4, Zhou Xiu-tian3   

  1. 1The Nursery College of Nanhua University, Hengyang 421001, Hunan Province, China
    2Hengyang Central Hospital, Hengyang 421008, Hunan Province, China
    3Medical College of Nanhua University, Hengyang 421001, Hunan Province, China
    4The First Affiliated Hospital of Nanhua University, Hengyang 421001, Hunan Province, China
  • Received:2012-03-01 Revised:2012-03-23 Online:2012-09-30 Published:2012-09-30
  • About author:Li Jian-zhi★, Master, Associate professor, the Nursery College of Nanhua University, Hengyang 421001, Hunan Province, China

PDF

453

被引次数

4

摘要：

背景：肾间质纤维化与组织基质金属蛋白酶2和金属蛋白酶组织抑制因子2表达失衡有关。
目的：建立单侧输尿管梗阻肾纤维化模型，观察川芎嗪治疗后肾间质病理变化及肾组织组织基质金属蛋白酶2和金属蛋白酶组织抑制因子2的表达变化。
方法：24只雌性SD大鼠分为3组，除假手术组外，模型组及川芎嗪组均在无菌条件下行左侧输尿管结扎建立单侧输尿管梗阻模型，川芎嗪组于术前1 d开始灌胃给药，40 mg/(kg•d)，1次/d，连续2周。术后14 d处死各组大鼠，留取梗阻侧肾组织行苏木精-伊红染色和Masson染色以观察肾组织病理改变，免疫组化和反转录-聚合酶链反应方法检测肾组织基质金属蛋白酶2、金属蛋白酶组织抑制因子2的表达水平。
结果与结论：川芎嗪组可明显减轻肾小管的扩张和萎缩，减轻肾间质纤维组织增生及炎性细胞浸润。与假手术组相比，模型组基质金属蛋白酶2、金属蛋白酶组织抑制因子2蛋白和mRNA基因表达均明显增加(P < 0.01)；川芎嗪组上述物质表达较模型组明显减少(P < 0.01)。提示川芎嗪通过下调基质金属蛋白酶2、金属蛋白酶组织抑制因子2的表达，从而改善单侧输尿管梗阻大鼠的肾间质纤维化。

关键词: 川芎嗪, 输尿管梗阻, 基质金属蛋白酶2, 金属蛋白酶组织抑制因子2, 肾间质纤维化

Abstract:

BACKGROUND: Renal interstitial fibrosis is related to the imbalance between matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinase-2.
OBJECTIVE: To establish the unilateral ureteral obstruction renal fibrosis model and to observe the pathological changes of renal interstitial and the expression of matrix metalloproteinase-2 and tissue inhibitor of matrix metalloproteinase-2 after treated with tetramethylpyrazine.
METHODS: Twenty-four female Sprague Dawley rats were randomly divided into three groups: sham-operation group, model group and tetramethylpyrazine group. The rats in the model group and tetramethylpyrazine group were performed with left ureter ligation to establish the unilateral ureteral obstruction model. Rats in the tetramethylpyrazine group were performed with intragastric administration at 1 day before surgery, 40 mg/(kg•d) once per day and lasted for 2 weeks. All rats were sacrificed at 14 days after surgery. The pathological changes of the obstruction renal tissues were examined by hematoxylin-eosin staining and Masson staining. Immunohistochemistry and reverse transcription-polymerase chain reaction were applied to detect the protein and mRNA expression of matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinase-2.
RESULTS AND CONCLUSION: Tetramethylpyrazine could significantly reduce the expansion and contraction of the tubular, the proliferation of renal interstitial fibrous tissue and inflammatory cell infiltration. Compared with sham-operation group, the protein and mRNA expression of matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinase-2 was significantly increased in model group (P < 0.01). The protein and mRNA expression of matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinase-2 in tetramethylpyrazine group was smaller than that in the model group (P < 0.01). Tetramethylpyrazineon could relieve the renal intersitial fibrosis by decreasing expression of matrix metalloproteinase-2 and tissue inhibitors of matrix metalloproteinase-2.

中图分类号: