中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (40): 7503-7507.doi: 10.3969/j.issn.2095-4344.2012.40.018

• 移植与中医药 transplantation and traditional Chinese medicine • 上一篇    下一篇

大黄酸可影响高糖培养SD大鼠肾小球系膜细胞的凋亡

郭 禹，李 岩，房 卉，印晓星，魏群利   

  1. 徐州医学院药学院，江苏省徐州市 221000
  • 收稿日期:2012-02-06 修回日期:2012-02-14 出版日期:2012-09-30 发布日期:2012-09-30
  • 通讯作者: 魏群利，博士，教授，主任医师，徐州医学院药学院，江苏省徐州市221000
  • 作者简介:郭禹★，女，1987年生，江苏省铜山区人，汉族，徐州医学院在读硕士，主要从事中药药理研究。

Rhein effects on apoptosis of glomerular mesangial cells cultured with high glucose in Sprague Dawley rats

Guo Yu, Li Yan, Fang Hui, Yin Xiao-xing, Wei Qun-li   

  1. College of Pharmacy, Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China
  • Received:2012-02-06 Revised:2012-02-14 Online:2012-09-30 Published:2012-09-30
  • Contact: Wei Qun-li, Doctor, Professor, Chief physician, College of Pharmacy, Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China 15077860033@139.com
  • About author:Guo Yu★, Studying for master’s degree, College of Pharmacy, Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China guoyu19861230@yahoo.com.cn

PDF

556

被引次数

4

摘要：

背景：含有大黄的复方中药消可宁能有效防治早期糖尿病肾病。
目的：观察大黄酸对髙糖培养的SD大鼠肾小球系膜细胞凋亡的影响。
方法：以20，40，80 μmol/L浓度的大黄酸刺激髙糖培养的肾小球系膜细胞，苏木精-伊红染色观察凋亡细胞形态，DAPI荧光染色观察细胞核凋亡情况，流式细胞仪观察细胞凋亡率。
结果与结论：苏木精-伊红染色及DAPI染色结果显示大黄酸可促进髙糖培养的肾小球系膜细胞的凋亡，且与浓度与时间成正相关。各组肾小球系膜细胞的细胞凋亡率差异有显著性意义(P < 0.05)，表明大黄酸对髙糖培养的肾小球系膜细胞凋亡产生影响且与浓度及时间成正相关。

关键词: 大鼠肾小球系膜细胞, 大黄酸, 细胞凋亡, 苏木精-伊红染色, DAPI荧光染色, 细胞凋亡率

Abstract:

BACKGROUND: Our previous study has already manifested that Chinese medicine Xiaokening containing Rhein can effectively control the development of early diabetic nephropathy.
OBJECTIVE: To investigate the effect of Rhein on apoptosis of glomerular mesangial cells (GMCs) of Sprague Dawley rats cultured with high glucose.
METHODS: The GMCs cultured with high glucose were stimulated by different concentrations of Rhein (20, 40, 80 μmol/L). Morphology of GMCs was discriminated by hematoxylin-eosin staining. Karyon apoptosis was examined by fluorescence staining of 4’,6-diamidino-2-phenylindole (DAPI). Cell apoptosis rate was detected by flow cytometry.
RESULTS AND CONCLUSION: The outcome of hematoxylin-eosin staining and 4’,6-diamidino-2-phenylindole staining indicated that Rhein could obviously induce apoptosis of GMCs in a dose- and time-dependent manner. The apoptosis rate had significant difference among different groups (P < 0.05). This result shows that Rhein can obviously induce apoptosis of GMCs cultured with high glucose and in a dose- and time-dependent manner.

中图分类号: