中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (37): 6867-6870.doi: 10.3969/j.issn.2095-4344.2012.37.006

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

实验性牙周炎大鼠模型的建立

孙继军，王 栋，王爱芹   

  1. 滨州医学院附属医院口腔内科，山东省滨州市 256603
  • 收稿日期:2011-12-21 修回日期:2012-02-01 出版日期:2012-09-09 发布日期:2012-09-09
  • 作者简介:孙继军★，女，1979年生，山东省德州市人，汉族，2005年山东大学毕业，硕士，讲师，主要从事牙周病学的基础和临床研究。 lena79315@163.com

Establishment of an experimental animal model of periodontitis in rats

Sun Ji-jun, Wang Dong, Wang Ai-qin   

  1. Department of Oral Medicine, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, Shandong Province, China
  • Received:2011-12-21 Revised:2012-02-01 Online:2012-09-09 Published:2012-09-09
  • About author:Sun Ji-jun★, Master, Lecturer, Department of Oral Medicine, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, Shandong Province, China lena79315@163.com

PDF

901

被引次数

10

摘要：

背景：建立实验性牙周炎大鼠模型是研究牙周病的基本方法之一，目前常用的建模方法存在许多弊端，需要进一步改进。
目的：建立一种近似于人类临床的牙周炎动物模型方法。
方法：将20只8周龄的Wistar大鼠随机分为实验组和对照组，锐分离实验组大鼠双侧下颌第一磨牙颊侧牙龈，局部黏结慢性牙周炎患者的牙石于牙颈部，滴入慢性牙周病炎患者的唾液10 μL/次，2次/d；对照组大鼠牙齿不做处理。两组大鼠均喂以高糖黏性食物。
结果与结论：造模14 d后实验组动物一般生物学特征和牙周组织病理、X射线变化均符合典型牙周炎表现。局部黏结牙石加混合细菌感染的方法建立的大鼠牙周炎模型可以模拟人类牙周炎的组织变化，是一种接近于人类临床的简单、实惠、有效的建模方法。

关键词: 牙周炎, 细菌感染, 牙石, 动物模型, 大鼠, 组织构建

Abstract:

BACKGROUND: To establish an experimental rat model of periodontitis is one of the basic methods to study periodontitis. However, the commonly used methods have many drawbacks and need further improvement.
OBJECTIVE: To investigate a new method that can establish an animal model of periodontitis similar to the human body.
METHODS: Twenty rats (8 weeks of age) were randomly divided into a control group and experimental group. In the experimental group, the buccolingual gingival of the first maxillary molar was sharply separated, dental calculi derived from chronic periodontitis patients were dried, crushed and adhered to the tooth neck; after that, 10 μL salivary of chronic periodontitis patients was dropped into the gingival twice every day. The contry group had no treatment. The amimals were all provided with high glucose food and drink.
RESULTS AND CONCLUSION: After 14 days of modeling, the general biological characteristics, pathological, and X-ray changes of the periodontal tissue of the model animals were dramatically in accord with typical human periodontitis. The rat model of periodontitis established by adhering dental calculus and mixed bacterial infection can simulate human periodontitis. It is a simple and effective method.

中图分类号: