中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (36): 6790-6794.doi: 10.3969/j.issn.2095-4344.2012.36.025

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

微小RNA -128b与-181a和-223在急性白血病患者中的表达

谭三勤1，2，王光平1，崔亚娟1，谢 熠3，谭宇婷2，陈方平1   

  1. 1中南大学湘雅医院血液科，湖南省长沙市 410008；
    2 湖南师范大学医学院，湖南省长沙市 410013；
    3 长沙市中心医院肿瘤科，湖南省长沙市 410004
  • 收稿日期:2011-12-01 修回日期:2011-12-16 出版日期:2012-09-02 发布日期:2012-09-02
  • 通讯作者: 陈方平，硕士，教授，中南大学湘雅医院血液科，湖南省长沙市 410008 xychenfp@2118.cn
  • 作者简介:谭三勤☆，女，1970年生，湖南省株洲市人，汉族，2010年中南大学毕业，博士，副教授，主要从事血液方面的研究。 tsqsghtz@yahoo.com.cn

Expression of microRNA-128b, -181 a and -223 in patients with acute leukemia

Tan San-qin1,2, Wang Guang-ping1, Cui Ya-juan1, Xie Yi3, Tan Yu-ting2, Chen Fang-ping1   

  1. 1Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China;
    2Medical College of Hunan Normal University, Changsha 410013, Hunan Province, China;
    3Department of Oncology, Changsha Central Hospital, Changsha 410004, Hunan Province, China
  • Received:2011-12-01 Revised:2011-12-16 Online:2012-09-02 Published:2012-09-02
  • Contact: Chen Fang-ping, Master, Professor, Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China xychenfp@2118.cn
  • About author:Tan San-qin☆, M.D., Associate professor, Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China; Medical College of Hunan Normal University, Changsha 410013, Hunan Province, China tsqsghtz@yahoo.com.cn

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415

被引次数

4

摘要：

背景：微小RNA是一类内源性非编码RNA。新近研究认为微小RNA可用作肿瘤标记物，对肿瘤进行分类。
目的：分析微小RNA -181a、微小RNA-128b和微小RNA-223在急性白血病患者中的表达差异。
方法：提取初治急性白血病患者及对照受试者骨髓单个核细胞总RNA，经微小RNA特异性引物反转录后，采用实时定量PCR分析各微小RNA的表达。
结果与结论：与对照受试者相比，无论是急性非淋巴细胞白血病还是急性淋巴细胞白血病患者骨髓单个核细胞的微小RNA-128b，微小RNA-181a和微小RNA-223表达均降低；统计学分析显示，急性非淋巴细胞白血病和急性白血病患者微小RNA-128b和微小RNA-181a表达与对照受试者比较差异有显著性意义(P < 0.05)，但急性淋巴细胞白血病患者与对照受试者比较差异无显著性意义(P > 0.05)；微小RNA-128b，-181a和-223的表达在急性非淋巴细胞白血病与急性淋巴细胞白血病患者之间差异无显著性意义(P > 0.05)。结果显示微小RNA-128b，-181a和-223有可能是急性白血病诊断与分型的生物标记物。

关键词: 微小RNA, 急性白血病, 表达, 骨髓单个核细胞, PCR

Abstract:

BACKGROUND: MicroRNA (miR) is a kind of endogenous non-coding RNA. Current studies have demonstrated that miR can be used as tumor marker for tumor categorization.
OBJECTIVE: To investigate the expressions of microRNA-181a, miR-128b and miR-223 and their significance in the plasma of patients with acute leukemia.
METHODS: The plasma RNAs were extracted from acute leukemia patients and normal control subjects, and were reverse transcribed with miR-specific primer into cDNA. The expressions of miR-181a, miR-128b and miR-223 were measured by real-time PCR.
RESULTS AND CONCLUSION: Compared with control subjects, the expression of miR-181a, miR-128b and miR-223 was decreased in acute non-lymphocytic leukemia and acute lymphoblastic leukemia patients. Statistical analysis indicated that the expression of miR-128b and miR-181 was significantly different between acute non-lymphocytic leukemia and acute leukemia patients and control subjects (P < 0.05), but its expression was not significantly different between acute lymphoblastic leukemia patients and conntrol subjects (P > 0.05). Compared with acute lymphoblastic leukemia patients, the expression of miR-223 was increased in acute non-lymphocytic leukemia patients, while miR-128b and miR-181a expressions were decreased. The expression of miR-181a, miR-128b and miR-223 was not significantly different between acute non-lymphocytic leukemia and acute lymphoblastic leukemia patients (P > 0.05). These findings suggest that miR-128b, miR-181a and miR-223 may be the novel biomarkers for the diagnosis of typing of acute leukemia.

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