中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (34): 6365-6371.doi: 10.3969/j.issn.2095-4344.2012.34.020

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

聚乳酸乙醇酸与猕猴骨髓基质细胞的生物相容性

胡 楠1，吴 红2，吴 坚2，丁 斐2，肖兆群2，顾晓松2   

  1. 1南通大学附属医院眼科，江苏省南通市 226001；
    2江苏省神经再生重点实验室，江苏省南通市 226001
  • 收稿日期:2012-02-10 修回日期:2012-04-15 出版日期:2012-08-19 发布日期:2012-08-19
  • 通讯作者: 顾晓松，博士，教授，江苏省神经再生重点实验室，江苏省南通市226001
  • 作者简介:胡楠☆，女，1965年生，江苏省无锡市人，汉族，1988年南通医学院毕业，博士，主任医师，主要从事神经保护和再生的研究

Biocompatibility of polylactic-co-glycolic acid with bone marrow stromal cells of rhesus monkeys in vitro

Hu Nan1, Wu Hong2, Wu Jian2, Ding Fei2, Xiao Zhao-qun2, Gu Xiao-song2   

  1. 1Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China;
    2Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu Province, China
  • Received:2012-02-10 Revised:2012-04-15 Online:2012-08-19 Published:2012-08-19
  • Contact: Gu Xiao-song, Doctor, Professor, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu Province, China neurongu@public.nt.js.cn,
  • About author:Hu Nan☆, Doctor, Chief physician, Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China huaneye@hotmail.com

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摘要：

背景：聚乳酸乙醇酸与不同细胞的生物相容性的报道较多，但探讨其与猕猴骨髓基质细胞生物相容性的研究极少。
目的：观察猕猴骨髓基质细胞的生物学特性以及其与支架材料聚乳酸乙醇酸在体外的生物相容性。
方法：将经流式细胞检测证实的第2代猕猴骨髓基质细胞与聚乳酸乙醇酸支架材料联合培养，以单独培养的骨髓基质细胞作为对照。
结果与结论：猕猴骨髓基质细胞与聚乳酸乙醇酸共培养4 d后，激光共聚焦显微镜下可见CD29阳性的骨髓基质细胞黏附包绕在聚乳酸乙醇酸纤维表面，形成长的细胞链。扫描电镜下可见骨髓基质细胞于聚乳酸乙醇酸上贴附良好，且有较多细长的突起从骨髓基质细胞伸出，沿材料伸展。骨髓基质细胞在聚乳酸乙醇酸浸出液中培养72 h，骨髓基质细胞神经营养因子表达量与对照组比较差异无显著性意义(P > 0.05)；培养7 d，其形态、细胞活力、细胞增殖指数与对照组亦无明显差异。提示猕猴骨髓基质细胞与聚乳酸乙醇酸支架材料有良好的生物相容性。

关键词: 骨髓基质细胞, 聚乳酸乙醇酸, 生物学特性, 生物相容性, 猕猴, 生物材料

Abstract:

BACKGROUND: Biocompatibility of polylactic-co-glycolic acid (PLGA) with different cells has been reported except its biocompatibility with rhesus monkey bone marrow stromal cells (BMSCs).
OBJECTIVE: To observe the biological characteristics of rhesus monkey BMSCs, and to investigate the biocompatibility of PLAG with rhesus monkey BMSCs.
METHODS: The second passage BMSCs of rhesus monkeys were co-cultured with PLGA for biocompatibility investigation. Light and electron microscopy revealed that BMSCs adhered to and elongated along the PLGA fiber scaffold. BMSCs cultured alone served as controls.
RESULTS AND CONCLUSION: After 4 days of co-culture with PLGA, CD29-positive BMSCs adhered to the surface of PLAG fiber scaffold, forming long cell chains. Some of the attached BMSCs elongated along the scaffold. After 72 hours culture in PLAG extract fluid, neurotrophic factors of BMSCs had no difference from those cultured in plain Iscove's Modified Dulbecco's Medium (P > 0.05). After 7 days, there were no differences in morphology, viability and proliferation of BMSCs cultured in PLGA extract fluid and plain Iscove's Modified Dulbecco's Medium. The results confirmed a high biocompatibility between PLGA materials and BMSCs.

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