中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (34): 6321-6325.doi: 10.3969/j.issn.2095-4344.2012.34.011

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

四甲基偶氮唑盐法评价牙体修复性纳米羟基磷灰石复合材料的体外细胞毒性

李 瑞1，王青山1，王 云2，赵蒙蒙1，王 爽1，张荣和1   

  1. 1滨州医学院附属医院口腔科，山东省滨州市 256603；
    2聊城市人民医院口腔内科，山东省聊城市 252000
  • 收稿日期:2012-02-04 修回日期:2012-03-27 出版日期:2012-08-19 发布日期:2012-08-19
  • 通讯作者: 王青山，教授，硕士生导师，滨州医学院附属医院口腔科，山东省滨州市256603 wqsyahao@ yahoo.com.cn
  • 作者简介:李瑞★，女，1986年生，山东省滨州市人，汉族，2012年滨州医学院毕业，硕士，医师，现于滨州市人民医院工作，主要从事牙体牙髓病学的研究。 lirui19860724@ 163.com

In vitro cytotoxicity of the self-made light-cured nano-hydroxyapatite composite for tooth restoration evaluated by MTT assay

Li Rui1, Wang Qing-shan1, Wang Yun2, Zhao Meng-meng1, Wang Shuang1, Zhang Rong-he1   

  1. 1Department of Stomatology, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong Province, China;
    2Department of Stomatology, People’s Hospital of Liaocheng City, Liaocheng 252000, Shandong Province, China
  • Received:2012-02-04 Revised:2012-03-27 Online:2012-08-19 Published:2012-08-19
  • Contact: Wang Qing-shan, Professor, Master’s supervisor, Department of Stomatology, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong Province, China wqsyahao@ yahoo.com.cn
  • About author:Li Rui★, Master, Physician, Department of Stomatology, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong Province, China Lirui19860724@ 163.com

PDF

413

被引次数

10

摘要：

背景：越来越多的新型材料被纳入口腔领域应用的视野，对生物材料进行生物相容性评价是进入临床试验前的重点研究内容。
目的：通过体外细胞毒性实验评价自制牙体修复性纳米羟基磷灰石复合材料的生物相容性。
方法：根据ISO标准，采用四甲基偶氮唑盐比色法行体外细胞毒性实验，阴性对照组为DMEM培养液，阳性对照组为含有0.1%苯酚的DMEM培养液，实验组为受试材料的浸提液。测试人牙龈成纤维细胞在牙体修复性纳米羟基磷灰石复合材料浸提液中培养1，3，5 d的吸光度值，观察细胞形态变化，计算细胞相对增殖率，判断细胞毒性的级别。
结果与结论：实验组体外细胞毒性实验吸光度值均与阴性对照组差异无显著性意义(P > 0.05)，与阳性对照组差异有显著性意义(P < 0.01)，自制牙体修复性纳米羟基磷灰石复合材料体外细胞毒性级别为0~1级，初步认为其具有良好的生物相容性。

关键词: 牙修复体, 纳米羟基磷灰石, 生物相容性, 四甲基偶氮唑盐法, 体外细胞毒性

Abstract:

BACKGROUND: More and more new materials are introduced in the field of oral application, and the evaluation of biomaterial biocompatibility is a key step before the clinical application
OBJECTIVE: To evaluate the biocompatibility of the self-made light-cured nano-hydroxyapatite composite for tooth restoration through in vitro cytotoxic experiments.
METHODS: According to ISO standards, in vitro cytotoxicity test (MTT assay) was used to test the absorbance value of human gingival fibroblasts in the self-made light-cured nano-hydroxyapatite composite for tooth restoration extracts (experimental group) of 1, 3, 5 days, to observe changes in cell morphology, to calculate the relative growth rate, and to determine the cytotoxicity grade. Cells in negative control group were cultured in Dulbecco’s modified Eagle’s medium, while those in positive control group were cultured in Dulbecco’s modified Eagle’s medium containing 0.1% phenol.
RESULTS AND CONCLUSION: The absorbance value in the experimental group was similar to that in the negative control group (P > 0.05), but significantly different from that in the positive control (P < 0.01), which indicated that the materials tested were safe to the human gingival fibroblasts. It is preliminarily estimated that nano-hydroxyapatite composite is a safe material for dental clinical application with good biocompatibility.

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