中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (34): 6326-6330.doi: 10.3969/j.issn.2095-4344.2012.34.012

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

普鲁兰基肿瘤靶向性纳米粒子的制备、稳定性和体外释放

唐红波1, 2，陈红丽3，周志敏2，张 彤2，刘玲蓉2，张其清2, 4   

  1. 1首都医科大学附属北京妇产医院药剂科，北京市 100007；
    2中国医学科学院生物医学工程研究所，天津市生物材料重点实验室，天津市 300192；
    3新乡医学院生命科学技术系，河南省遗传性疾病与分子靶向药物重点实验室培育基地，河南省新乡市 453003；
    4厦门大学材料学院生物材料系/生物医学工程研究中心/福建省生物医学工程重点实验室/厦门市生物医学工程技术研究中心，福建省厦门市 361005
  • 收稿日期:2012-02-05 修回日期:2012-06-28 出版日期:2012-08-19 发布日期:2012-08-19
  • 通讯作者: 张其清，博士，教授，博士生导师，中国医学科学院生物医学工程研究所，天津市生物材料重点实验室，天津市 300192；厦门大学材料学院生物材料系/生物医学工程研究中心/福建省生物医学工程重点实验室/厦门市生物医学工程技术研究中心，福建省厦门市 361005 zhangqiq@ 126.com
  • 作者简介:唐红波☆，女，1977年生，湖南省长沙市人，2010年北京协和医学院毕业，博士，主管药师，主要从事妇科肿瘤靶向纳米药物和医院药学方面的研究。 tanghongbo77@gmail.com

Tumor-targeted nanoparticles derived from pullulan acetate conjugate: Preparation, stability and release in vitro

Tang Hong-bo1, 2, Chen Hong-li3, Zhou Zhi-min2, Zhang Tong2, Liu Ling-rong2, Zhang Qi-qing2, 4   

  1. 1Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100007, China;
    2Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China;
    3The Key Laboratory of Hereditary Disease and Molecular Target Drug Therapy, Department of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan Province, China;
    4Research Center of Biomedical Engineering, Medical College of Xiamen University, Xiamen 361005, Fujian Province, China
  • Received:2012-02-05 Revised:2012-06-28 Online:2012-08-19 Published:2012-08-19
  • Contact: Zhang Qi-qing, Doctor, Professor, Doctoral supervisor, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China; Research Center of Biomedical Engineering, Medical College of Xiamen University, Xiamen 361005, Fujian Province, China zhangqiq@126.com
  • About author:Tang Hong-bo☆, Doctor, Pharmacist in charge, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100007, China; Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China tanghongbo77@ gmail.com

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421

被引次数

6

摘要：

背景：普鲁兰多糖以其独特的优点在纳米递药系统领域受到越来越多的关注，但是，以普鲁兰多糖为材料进行改性制备的肿瘤靶向的纳米药物载体仍有待进一步研究与开发。
目的：观察纳米粒子和载药纳米粒子的体外稳定性及所包载药物的释放特征，初步评价其作为纳米药物载体的潜力。
方法：应用透析法制备乙酰普鲁兰叶酸偶合体纳米粒子，以表阿霉素为模型药物，制备乙酰普鲁兰叶酸偶合体/表阿霉素载药纳米粒子(FPA/EPI)，应用储存法考察其稳定性，应用透析袋法观测体外释放特征。
结果与结论：乙酰普鲁兰叶酸偶合体纳米粒子和FPA/EPI的粒径分别为(204.2±10.9) nm 和(273.4±11.0) nm，在蒸馏水和体积分数10%胎牛血清中表面电位均较低，乙酰普鲁兰叶酸偶合体纳米粒子在水溶液中粒径1年内未见显著改变。载药纳米粒子对所包载的药物表阿霉素进行很好地释放，pH 5.0磷酸盐缓冲液中释放速度明显高于pH 7.4；乙酰普鲁兰叶酸偶合体纳米粒子和FPA/EPI制备容易，稳定性好，初步说明了两种粒子可望成为新型肿瘤靶向药物递药系统。

关键词: 乙酰普鲁兰, 叶酸受体, 纳米粒子, 稳定性, 体外释放

Abstract:

BACKGROUND: Pullulan due to its many unique characteristics have received more and more attention in the field of drug delivery systems. But, the tumor targeted nano-drug carriers based on pullulan needed to be further studied and developed.
OBJECTIVE: To observe the stability and drug release in vitro of nano-drug carriers and to preliminarily evaluate the potential of folate conjugated pullulan acetate (FPA) as a nano-drug carrier.
METHODS: Folate was coupled to pullulan acetate (PA). FPA nanoparticles (FPAN) and epirubicin-loaded FPA nanoparticles (FPA/EPI) were prepared by dialysis method. The storage stability of FPAN and FPA/EPI was observed by storage method, and the in-vitro release characteristics were studied by dialysis bag method.
RESULTS AND CONCLUSION: FPAN and FPA/EPI had the nearly spherical shape with a size range of (204.2±10.9) nm and (273.4±11.0) nm, respectively, and they had low ζ potentials both in water and in 10% fetal bovine serum. FPAN maintained stable for at least 1 year. The drug encapsulated in FPAN was released more quickly in pH 5.0 PBS than in pH 7.4. It is concluded that the FPA nano-drug carrier is easy to prepare and has good stability. FPA and FPA/EPI nanoparticles have the potential to be new tumor-targeted nano-drug delivery systems.

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