中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (27): 5126-5130.doi: 10.3969/j.issn.2095-4344.2012.27.035

• 干细胞学术探讨 stem cell academic discussion • 上一篇    

表皮干细胞在组织工程创面修复中的应用

吕淑坤   

  1. 齐齐哈尔市第一医院皮肤科，黑龙江齐齐哈尔市 161000
  • 收稿日期:2012-03-03 修回日期:2012-05-13 出版日期:2012-07-01 发布日期:2013-11-01
  • 作者简介:吕淑坤，女，1975年生，黑龙江省齐齐哈尔市人，齐齐哈尔市第一医院皮肤科，2000年齐齐哈尔医学院毕业，主要从事皮肤创面愈合方面的研究。 doctor123sunfa@126.com

Epidermal stem cells in wound repair by tissue engineering

Lü Shu-kun   

  1. Department of Skin, First Hospital of Qiqihar, Qiqihar 161000, Heilongjiang Province, China
  • Received:2012-03-03 Revised:2012-05-13 Online:2012-07-01 Published:2013-11-01
  • About author:Lü Shu-kun, Department of Skin, First Hospital of Qiqihar, Qiqihar 161000, Heilongjiang Province, China doctor123sunfa@126.com

PDF

473

被引次数

24

摘要：

背景：随着细胞生物学、分子生物学技术、组织工程学的快速发展，探寻组织工程化皮肤创面覆盖物的“种子细胞”的研究逐渐增多。
目的：总结表皮干细胞的生物学特性，探讨其在皮肤创面修复过程中的再生作用与临床应用价值。
方法：应用计算机检索CNKI和PubMed数据库中2002-07/2011-12关于表皮干细胞修复皮肤损伤研究的文章，以“表皮干细胞，创面修复，组织工程，皮肤”或“epidermal stem cells，tissue engineering skin，wound surface”为检索词进行检索。选择文章内容与表皮干细胞修复皮肤损伤研究进展有关的文章，同一领域文献则选择近期发表或发表在权威杂志文章。初检得到129篇文献，根据纳入标准选择28篇文献进行综合分析。
结果与结论：表皮干细胞是表皮发生、分化和创面修复的基础，其正常增殖分化是维持皮肤正常组织结构和细胞内环境稳定的基本要求，也是皮肤组织工程理想的种子细胞。对烧伤、创伤等大面积皮肤缺损的治疗，对皮肤疾病的细胞疗法、基因治疗等方面都有很好的应用前景。表皮细胞的体外培养是复合人工皮肤组织工程学研究的先决条件。随着对表皮干细胞分离、纯化和培养技术的不断完善，可达到迅速构建表皮层的目的。但表皮干细胞的应用研究仍需要进一步的探索。

关键词: 表皮干细胞, 烧伤, 创面修复, 组织工程, 皮肤, 干细胞

Abstract:

BACKGROUND: With rapid development of cell biology, molecular biology technique, and tissue engineering, increasing studies focus on seed cells for constructing skin wound surface by tissue engineering.
OBJECTIVE: To summarize the biological characteristics of epidermal stem cells and investigate their regenerative and clinical application value in repair of skin wound surface.
METHODS: A computer-based online retrieval of SNKI and PubMed database for searching papers describing epidermal stem cells for repair of skin injury using the key words of “epidermal stem cells, tissue engineering skin, wound surface” in English and Chinese. Papers related to research progress in epidermal stem cells for repair of skin injury were selected. Papers that were published recently in the same research field or in high-impact journals were selected. A total of 129 papers were initially retrieved. According to inclusion criteria, 25 papers were included for final analysis.
RESULTS AND CONCLUSION: Epidermal stem cells support for epidermal generation, differentiation and wound healing. Epidermal stem cells provide the condition for maintaining skin normal structure and intracellular environment due to their normal proliferation and differentiation and are the ideal seed cells of skin tissue engineering. They show satisfactory prospects in treatment of large-area skin defects, cell therapy of skin disease, and gene therapy. In vitro culture of epidermal stem cells is a prerequisite for studying tissue engineering of artificial skin. As the isolation, purification and culture technology of epidermal stem cells continues to improve, we can quickly build epidermis. Nevertheless, application of epidermal stem cells requires further investigation.

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