中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (27): 5006-5010.doi: 10.3969/j.issn.2095-4344.2012.27.013

• 干细胞转基因表达 transgenic expression in stem cells • 上一篇    下一篇

慢性粒细胞白血病患者骨髓来源Flk1+CD31-CD34-间充质干细胞中 BCR/ABL融合基因的检测

朱希山，张 舸，施 薇，台卫平   

  1. 首都医科大学附属北京世纪坛医院(北京大学第九临床医学院)，肿瘤内科，北京市 100038
  • 收稿日期:2011-11-23 修回日期:2011-12-16 出版日期:2012-07-01 发布日期:2013-11-01
  • 作者简介:朱希山☆，男，山东省潍坊市人，汉族，2009年北京协和医学院(清华大学医学部)毕业，博士，医师，主要从事干细胞基础研究与临床应用。 zhuxishan@tsinghua.org.cn

Detection of BCR/ABL fusion gene in bone marrow-derived Flk1+CD31-CD34- mesenchymal stem cells from chronic myelogenous leukemia patients

Zhu Xi-shan, Zhang Ge, Shi Wei, Tai Wei-ping   

  1. Department of Medical Oncology, Affiliated Beijing Millennium Monument Hospital of Capital Medical University (Ninth Clinical Medical College of Peking University), Beijing 100038, China
  • Received:2011-11-23 Revised:2011-12-16 Online:2012-07-01 Published:2013-11-01

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摘要：

背景：免疫表型为Flk1+CD31-CD34-的间充质干细胞体内外实验研究均证实在单细胞水平可以向造血及内皮细胞分化。
目的：观察慢性粒细胞白血病患者骨髓BCR/ABL融合基因的表达情况。
方法：体外培养扩增慢性粒细胞白血病患者骨髓来源原始间充质干细胞，测定其生长曲线，分析其细胞周期及免疫表型，使用RT-PCR和FISH的方法检测其BCR/ABL融合基因的表达情况。
结果与结论：慢性粒细胞白血病患者骨髓来源的原始间充质干细胞呈成纤维样生长，大部分细胞处于G0/G1期，并且高表达Flk1，CD13，CD29，CD44，用RT-PCR和FISH的方法能够检测出BCR/ABL融合基因的表达。提出慢性粒细胞白血病的白血病基因转化可能发生在比造血干细胞更高的血液血管干细胞水平上。

关键词: 慢性粒细胞白血病, 血液血管干细胞, 分化, 基因, CD13, CD29, CD44, 干细胞

Abstract:

BACKGROUND: The in vivo and in vitro experiment has confirmed that Flk1+CD31-CD34- mesenchymal stem cells can differentiate into hematopoietic and endothelial cells at the single cells level.
OBJECTIVE: To observe the expression of BCR/ABL fusion gene from the bone marrow of chronic myelogenous leukemia patients.
METHODS: The primitive bone marrow-derived mesenchymal stem cells from chronic myelogenous leukemia patients were cultured and amplified in vitro. The growth curve was detected and the cell cycle and immune phenotype were analyzed. The expression of BCR/ABL fusion gene was detected by RT-PCR and FISH.
RESULTS AND CONCLUSION: The primitive bone marrow-derived mesenchymal stem cells from chronic myelogenous leukemia patients showed a fibroblast-like morphology, most of these cells were in the G0/G1 phases and had high expansion of Flk1, CD13, CD29, CD44. The expression of BCR/ABL fusion gene could be identified by RT-PCR and FISH. The transformation of leukemia gene in chronic myelogenous leukemia may occur on blood cells and endothelial cells which were higher than hemangioblastic cells.

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