中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (27): 4996-5000.doi: 10.3969/j.issn.2095-4344.2012.27.011

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

快速浓集自体脂肪干细胞促进移植异体骨早期血管化

殷 剑1，杨 毅1，杨小丰2，曹 鑫1，邓 伟1，邓 强1，柴黎明2，金格勒1   

  1. 1新疆医科大学第一附属医院，新疆维吾尔自治区乌鲁木齐市 830054；
    2新疆自治区人民医院，新疆维吾尔自治区乌鲁木齐市 830001
  • 收稿日期:2012-03-05 修回日期:2012-04-20 出版日期:2012-07-01 发布日期:2013-11-01
  • 通讯作者: 金格勒，博士，博士生导师，主任医师，教授，新疆医科大学第一附属医院，新疆维吾尔自治区乌鲁木齐市 830054 jingl81@yahoo.com.cn
  • 作者简介:殷剑★，男，1983年生，新疆维吾尔自治区霍城县人，汉族，新疆医科大学在读硕士，主要从事脊柱外科和骨组织工程方面的研究。 qsyj1983@163.com
  • 基金资助:

    新疆维吾尔自治区自然科学基金面上项目(2010211A40)，高校博士学科点专项基金(20116517120001) ，新疆医科大学硕士研究生创新基金(MC2010-18)，新疆医科大学第一附属医院组织工程专项基金(201022GC04)。

Rapid accumulation of autologous adipose-derived stem cells promotes early vascularization of implanted allogeneic bone

Yin Jian1, Yang Yi1, Yang Xiao-feng2, Cao Xin1, Deng Wei1, Deng Qiang1, Chai Li-ming2, Jin Ge-le1   

  1. 1First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China;
    2People’s Hospital of Xinjiang Uyghur Autonomous Region, Urumqi 830001, Xinjiang Uyghur Autonomous Region, China
  • Received:2012-03-05 Revised:2012-04-20 Online:2012-07-01 Published:2013-11-01
  • Contact: Jin Ge-le, M.D., Doctoral supervisor, Chief physician, Professor, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uyghur Autonomous Region, China jingl81@yahoo.com.cn

PDF

444

被引次数

5

摘要：

背景：目前可满足骨修复的组织工程骨的快速构建方法尚不成熟，植入物早期血管化仍是研究热点。
目的：将快速浓集自体脂肪干细胞直接置于植骨周围，通过正电子发射型计算机断层显像/CT直观观察其对植入异体骨的早期血管化影响，从而探索一种可用于临床的快速组织工程骨构建方法。
方法：将45只雄性新西兰大白兔随机等分为异体骨组、复合组和自体骨组，分别在腰5，6横突间植入同种异体髂骨条、同种异体髂骨条复合快速浓集自体脂肪干细胞和自体髂骨条，各组于植入后1，3，5周注射18F-NaF，通过正电子发射型计算机断层显像/CT对各组动物进行扫描显像，对比各组植骨区标准摄取值。
结果与结论：异体骨兔植骨区中段放射性分布低，血供及代谢未见明显改善，而复合快速浓集自体脂肪干细胞组植骨区18F-NaF的标准摄取值均不同程度地高于异体骨组(P < 0.05)，提示快速浓集自体脂肪干细胞可促进植入骨的早期血管化，有效改善植骨区的血供和代谢，为临床快速构建组织工程骨提供了一种新思路。

关键词: 血管化, 脂肪干细胞, 同种异体骨, 自体骨, 组织工程, 干细胞

Abstract:

BACKGROUND: It is not yet available to construct tissue-engineered bone quickly for repair of bone defects in the clinic, and early vascularization of the implants remains to be paid increasing attention.
OBJECTIVE: The rapidly accumulated autologous adipose-derived stem cells were directly placed around the implants to investigate the effects on early vascularization of implants using positron emission tomography PET/CT and to search a method to rapidly construct tissue-engineered bone in the clinic.
METHODS: Forty-five male New Zealand rabbits were randomly and evenly divided into three groups. These groups were implanted respectively in the lumbar 5,6 intertransverse space with allograft iliac bone (allograft group), allograft iliac bone composited with rapidly accumulated adipose-derived stem cells (compound group), and autologous iliac bone (autograft group). Each group was injected with 18F-NaF at 1, 3, 5 weeks post-surgery and detected by positron emission tomography PET/CT to contrast the standard uptake value.
RESULTS AND CONCLUSION: At 1, 3, 5 weeks post-surgery, the distribution of radioactivity in the middle-segment of allogeneic bone was low, with poor improvement in blood supply and metabolism. The standard uptake value of 18F-NaF in the autograft group and compound group was significantly higher than that in the allograft group respectively (P < 0.05). It is demonstrated that rapid accumulation of autologous adipose-derived stem cells may promote early vascularization of allogeneic bone and improve the blood supply and metabolism in the implanted area. It is also a novel idea to rapidly construct tissue-engineered bone in the clinic.

中图分类号: