中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (27): 4963-4967.doi: 10.3969/j.issn.2095-4344.2012.27.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

损伤肝组织匀浆与骨髓间充质干细胞的分化

侯军霞1，梅浙川1，蒋明德2   

  1. 1重庆医科大学附属第二医院消化内科，重庆市 400010；
    2解放军成都军区总医院消化内科，四川省成都市 610083
  • 收稿日期:2012-03-01 修回日期:2012-04-24 出版日期:2012-07-01 发布日期:2013-11-01
  • 通讯作者: 梅浙川，硕士，硕士生导师，教授，主任医师，重庆医科大学附属第二医院消化内科，重庆市 400010 meizhechuan@21cn.com
  • 作者简介:侯军霞★，女，1985年生，河北省邢台市人，2012年重庆医科大学毕业，硕士，主要从事骨髓间充质干细胞治疗肝病方面的研究。 houjunxia2009@163.com

Effects of tissue homogenate from injured liver on induced differentiation of bone marrow mesenchymal stem ce

Hou Jun-xia1, Mei Zhe-chuan1, Jiang Ming-de3   

  1. 1Department of Gastroenterology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China;
    2Department of Gastroenterology, Chengdu Military General Hospital, Chengdu 610083, Sichuan Province, China
  • Received:2012-03-01 Revised:2012-04-24 Online:2012-07-01 Published:2013-11-01
  • Contact: Mei Zhe-chuan, Master, Master’s supervisor, Professor, Chief physician, Department of Gastroenterology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China meizhechuan@21cn.com

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摘要：

背景：目前认为，骨髓间充质干细胞能否分化为肝细胞与微环境关系密切。
目的：探讨急性酒精性肝损伤组织匀浆对骨髓间充质干细胞的诱导分化作用。
方法：分别用正常和损伤肝脏组织匀浆诱导骨髓间充质干细胞分化，在第0，3，7，10，14，21天用RT-PCR和Western blot法检测甲胎蛋白、白蛋白的表达。
结果与结论：骨髓间充质干细胞在正常及损伤的肝脏组织匀浆诱导下均表现为类上皮样改变。两组均从第3天开始检测到甲胎蛋白的表达，并在第7天左右达到高峰，之后表达逐渐减少，第14天时量很少；在第7天检测到白蛋白的表达，且随时间延长表达量逐渐增加，mRNA水平与蛋白水平表达一致。表明肝细胞在诱导培养下逐渐从幼稚到成熟。但正常和损伤肝组织匀浆诱导的肝细胞甲胎蛋白和白蛋白的表达量差异无显著性意义(P > 0.05)。结果说明急性酒精性肝损伤组织匀浆可以诱导骨髓间充质干细胞向肝细胞分化，其诱导效果与正常肝脏组织匀浆相近。

关键词: 肝组织匀浆, 骨髓间充质干细胞, 细胞分化, 肝细胞, 酒精性肝损伤, 干细胞

Abstract:

BACKGROUND: Whether bone marrow mesenchymal stem cells can differentiate into hepatocytes is closely related to microenvironment.
OBJECTIVE: To explore the effects of the acute alcoholic liver injury tissue homogenate on induced differentiation of bone marrow mesenchymal stem cells.
METHODS: BMSCs were isolated from Sprague-Dawley rats, cultured, purified and identified in vitro. Rat models of alcohol-induced acute liver injury were prepared. Tissue homogenate of normal and injured liver was used to induce differentiation of bone marrow mesenchymal stem cells. The α-fetoprotein and albumin expression was detected at 0, 3, 7, 10, 14, and 21 days using RT-PCR and western blot methods, respectively.
RESULTS AND CONCLUSION: After induced by tissue homogenate of normal and injured liver, bone marrow mesenchymal stem cells exhibit epithelioid change. After induced by each tissue homogenate, α-fetoprotein expression was detected from the 3rd day, peaked at about 7 days, decreased thereafter, and exhibited a very low level by 14 days; albumin expression was detected from the 7th day, and the expression level was gradually increased with time going. The mRNA and protein expression level was consistent. These findings demonstrate that hepatocytes gradually developed till mature. There was no significant difference in α-fetoprotein or albumin expression after induced by tissue homogenate of normal and injured liver (P > 0.05). The liver tissue homogenates of acute alcoholic liver injury can induce bone marrow mesenchymal stem cells to differentiate into hepatocytes, with similar effects to normal liver tissue homogenate.

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