中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (11): 1711-1716.doi: 10.3969/j.issn.2095-4344.1117

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

低频脉冲电磁场促进周围神经损伤模型大鼠延迟修复后神经功能的恢复

张理乾1,徐春归1,李子煜1,姚  飞1,查小伟1,祁  雷2,荆珏华1   

  1. (1安徽医科大学第二附属医院骨科,安徽省合肥市  230000;2南京市浦口医院骨科,江苏省南京市  211800)
  • 收稿日期:2018-11-28 出版日期:2019-04-18 发布日期:2019-04-18
  • 通讯作者: 荆珏华,博士生导师,教授,主任医师,安徽医科大学第二附属医院,安徽省合肥市 230000
  • 作者简介:张理乾,男,1993年生,安徽省合肥市人,汉族,安徽医科大学在读硕士,主要从事周围神经损伤方面研究。 并列第一作者,徐春归,男,1984年生,安徽省合肥市人,汉族,2014年北京大学医学部毕业,博士,主要从事周围神经损伤方面研究。

Low-frequency pulsed electromagnetic field promotes neurologic function recovery after delayed repair of perioheral nerve injury in rats

Zhang Liqian1, Xu Chungui1, Li Ziyu1, Yao Fei1, Zha Xiaowei1, Qi Lei2, Jing Juehua1   

  1. (1Department of Orthopedics, the Second Hospital of Anhui Medical University, Hefei 230000, Anhui Province, China; 2Department of Orthopedics, Pukou Hospital of Nanjing City, Nanjing 211800, Jiangsu Province, China)
  • Received:2018-11-28 Online:2019-04-18 Published:2019-04-18
  • Contact: Jing Juehua, Doctoral supervisor, Professor, Chief physician, Department of Orthopedics, the Second Hospital of Anhui Medical University, Hefei 230000, Anhui Province, China
  • About author:Zhang Liqian, Master candidate, Department of Orthopedics, the Second Hospital of Anhui Medical University, Hefei 230000, Anhui Province, China Xu Chungui, MD, Department of Orthopedics, the Second Hospital of Anhui Medical University, Hefei 230000, Anhui Province, China Zhang Liqian and Xu Chungui contributed equally to this work.

摘要:

文章快速阅读:

文题释义:
低频脉冲电磁场:是指频率1-100 Hz,强度低于10 mT的低频、低强度调制磁场,在保留静态磁场治疗作用的基础上,使磁疗辐射产生强度可调的交变脉冲动态磁场;动态磁场强度可从5到100 Hz范围内调节,充分发挥出各个频率磁场的磁疗作用;不同的电磁场强度和频率有不同的生物效应。低频脉冲电磁场作用于神经组织,不产生热效应,而产生类似于流体机械塑形的作用,并通过不对称的宽幅脉冲影响许多异常的生物过程,进而改善神经细胞的病理状态,抑制神经细胞凋亡,促进神经生长。
周围神经损伤的延迟修复:创伤造成的周围神经损伤大多数无法Ⅰ期修复,因需优先处理危及生命的损伤,等待生命体征平稳后再进行Ⅱ期手术修复周围神经的损伤。
摘要
背景
:低频脉冲电磁场可以促进周围神经损伤的即时修复,但其对于延迟修复的影响尚不清楚。
目的:探讨低频脉冲电磁场是否可以促进周围神经损伤延迟修复后的神经功能恢复。
方法:将60只大鼠构建周围神经损伤模型,将模型大鼠随机分为低频脉冲电磁场治疗组及神经损伤延迟修复组,30只大鼠构建假手术模型(假手术组)。低频脉冲电磁场治疗组的模型大鼠在进行坐骨神经离断并行延迟修复后予以低频脉冲电磁场刺激;神经损伤延迟修复组仅进行坐骨神经离断及延迟修复处理;假手术组仅暴露坐骨神经不做离断。
结果与结论:与神经损伤延迟修复组相比,低频脉冲电磁场治疗组模型大鼠坐骨神经指数修正因子明显上升,Western blot结果显示坐骨神经组织中脑源性神经营养因子及血管内皮生长因子表达水平显著增加,免疫组化结果显示低频脉冲电磁场治疗组坐骨神经中的细胞再生数量增多。提示低频脉冲电磁场可以促进周围神经损伤模型大鼠延迟修复后的神经功能恢复。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0002-4402-5344(荆珏华)

关键词: 低频脉冲电磁场, 延迟修复, 周围神经, 神经离断, 神经再生, 功能恢复, 坐骨神经功能指数, 免疫组化, 组织构建

Abstract:

BACKGROUND: Low-frequency pulsed electromagnetic fields can promote the immediate repair of peripheral nerve injury, but its effect on delayed repair is still unclear.
OBJECTIVE: To explore whether low-frequency pulsed electromagnetic fields can promote the recovery of neurologic function in rats after delayed repair of peripheral nerve injury.
METHODS: Sixty rats were used for constructing the model of peripheral nerve injury, and then the rat models were randomized into low-frequency pulsed electromagnetic field treatment and delayed repair groups, and another 30 rats were included in the sham operation group. The rats in the low-frequency pulsed electromagnetic field treatment group received low-frequency pulsed electromagnetic fields after sciatic nerve disconnection and delayed repair intervention. The delayed repair group received sciatic nerve disconnection and delayed repair intervention. The sham operation group only exposed the sciatic nerve without disconnection.
RESULTS AND CONCLUSION: The sciatic functional index in the low-frequency pulsed electromagnetic field treatment group was significantly higher than that in the delayed repair group. Western blot assay results showed that the expression levels of brain-derived neurotrophic factor and vascular endothelial growth factor in the sciatic nerve were significantly increased. Immunohistochemistry revealed that the number of sciatic nerve cells in the low-frequency pulsed electromagnetic field treatment group was significantly increased. In summary, low-frequency pulsed electromagnetic fields can promote neurologic function recovery after delayed repair of peripheral nerve injury in rats.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Electromagnetic Fields, Sciatic Nerve, Tissue Engineering

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