中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (14): 2227-2232.doi: 10.3969/j.issn.2095-4344.0738

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

阴极弧沉积掺硅二氧化锆膜对MG63细胞相关因子的影响

章筛林，成翔宇，石继祥，周  强，石文俊，刘孚瑛，纪  斌   

  1. 上海中医药大学附属普陀医院骨科，上海市 200062
  • 收稿日期:2017-12-26 出版日期:2018-05-18 发布日期:2018-05-18
  • 通讯作者: 纪斌，主任医师，硕士生导师，上海中医药大学附属普陀医院骨科，上海市 200062
  • 作者简介:章筛林，男，1977年生，汉族，2013年苏州大学毕业，博士，主治医师，主要从事创伤骨科、关节外科研究。
  • 基金资助:

    上海市普陀区卫生系统自主创新项目(2013PTKW015)；国家自然科学基金项目(81673782)；上海市普陀区临床重点专科建设项目(2016PTZK04)

Osteoclast activity of MG63 cells on silicon doped zirconia films prepared by cathodic arc deposition

Zhang Shai-lin, Cheng Xiang-yu, Shi Ji-xiang, Zhou Qiang, Shi Wen-jun, Liu Fu-ying, Ji Bin   

  1. Department of Orthopaedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
  • Received:2017-12-26 Online:2018-05-18 Published:2018-05-18
  • Contact: Ji Bin, Chief physician, Master’s supervisor, Department of Orthopaedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
  • About author:Zhang Shai-lin, M.D., Attending physician, Department of Orthopaedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China
  • Supported by:

    Independent Innovation Project of Shanghai Putuo District Health System, No. 2013PTKW015; National Natural Science Foundation of China, No. 81673782; Key Clinical Specialist Construction Programs of Putuo District, Shanghai, No. 2016PTZK04

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摘要：

文章快速阅读：

 

文题释义：
氧化锆陶瓷：具有良好的韧性、强度及优良的生物相容性，弹性模量与不锈钢接近，成为一种优良的生物陶瓷材料。
磁过滤真空阴极弧沉积：具有离子化率高、离子能量大、绕射性能好、沉积速度快、可镀材料广等优点，适用于绝大多数金属与合金材料，在薄膜沉积技术中得到了广泛应用。研究表明，采用真空磁过滤阴极弧沉积技术可在钛片表面制备均匀、致密的氧化锆膜，而且制备的氧化锆膜可提高钛片表面的生物相容性及成骨细胞活性。
 
 
背景：采用各种表面改性方法提高医用钛及钛合金植入物的骨整合，是目前人工关节假体领域研究的重点之一。
目的：在纯钛表面制备掺硅二氧化锆膜，研究其对成骨样MG63细胞相关因子的影响。
方法：采用磁过滤真空阴极弧沉积技术在纯钛表面分别制备掺硅二氧化锆膜与二氧化锆膜。将成骨样MG63细胞分别接种于掺硅二氧化锆膜、二氧化锆膜及纯钛片表面，接种后第1，4，7，10天，以定量RT-PCR法检测细胞骨保护素和核因子κB受体活化因子配体基因表达情况，ELISA检测细胞分泌骨保护素和核因子κB受体活化因子配体蛋白量。

结果与结论：①接种第1天，3组骨保护素基因及蛋白表达无差异；接种第4天，掺硅二氧化锆膜组、二氧化锆膜组骨保护素基因及蛋白表达高于纯钛组(P < 0.05)，掺硅二氧化锆膜组、二氧化锆膜组骨保护素基因及蛋白表达无差异；接种第7，10天，掺硅二氧化锆膜组骨保护素基因及蛋白表达高于二氧化锆膜组、纯钛组(P < 0.05)；②接种第1天，3组核因子κB受体活化因子配体基因及蛋白表达无差异；接种第4，7天，掺硅二氧化锆膜组核因子κB受体活化因子配体基因及蛋白表达低于二氧化锆膜组、纯钛组(P < 0.05)；接种第10天，掺硅二氧化锆膜组、二氧化锆膜组基因及蛋白表达低于纯钛组(P < 0.05)，掺硅二氧化锆膜组、二氧化锆膜组骨保护素基因及蛋白表达无差异；③结果表明，在纯钛表面阴极弧沉积掺硅二氧化锆膜，能够上调成骨样MG63细胞内骨保护素表达水平，同时下调核因子κB受体活化因子配体表达水平。

ORCID: 0000-0002-9806-0292(纪斌)

关键词: 真空阴极弧沉积, 二氧化锆, 硅, 生物材料, 成骨细胞, 骨保护素, 核因子κB受体活化因子配体, 国家自然科学基金

Abstract:

BACKGROUND: Various surface modification techniques have been utilized as an attempt to improve the osteointegration of Ti-based implants, which is a hotspot research concerning the artificial joint prosthesis.

OBJECTIVE: To evaluate the effect of silicon doped zirconia film on the titanium surface on relevant factors of osteoblast-like MG63 cells.

METHODS: Silicon doped zirconia and zirconia films were respectively prepared on the titanium surface by cathodic arc deposition. Osteoblast-like MG63 cells were cultured on silicon doped zirconia film, zirconia film and pure titanium, respectively. After 1, 4, 7, 10 days of incubation, samples were collected for assay. Osteoprotegerin (OPG) and receptor activator of nuclear factor κB ligand (RANKL) at mRNA and protein levels were detected using real-time PCR and ELISA, respectively.

RESULTS AND CONCLUSION: (1) At 1 day of inoculation, there was no significant difference in the OPG mRNA and protein expression among the three groups. At 4 days of inoculation, the expression of OPG mRNA and protein in the silicon doped zirconia film and zirconia film groups was significantly higher than that in the pure titanium group (P < 0.05), but there was no significant difference between the former two groups. At 7 and 10 days of inoculation, the expression of OPG mRNA and protein in the silicon doped zirconia film growp was highest among the three groups (P < 0.05). (2) At 1 day of inoculation, there was no significant difference in the RANKL mRNA and protein expression among the three groups. At 4 and 7 days of inoculation, the expression of RANKL mRNA and protein in the silicon doped zirconia film group was significantly lower than that in the other two groups (P < 0.05). At 10 days of inoculation, the expression of OPG mRNA and protein in the silicon doped zirconia film and zirconia film groups was significantly higher than that in the pure titanium group (P < 0.05), but there was no significant difference between the former two groups. To conclude, silicon doped zirconia film fabricated by cathodic arc deposition can enhance the expression of OPG and reduce the expression of RANKL at the same time.

Key words: Titanium, Osteoblasts, Osteoprotegerin, Receptor Activator of Nuclear Factor-kappa B, Tissue Engineering

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