中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (2): 323-328.doi: 10.3969/j.issn.2095-4344.0025

• 生物材料综述 biomaterial review • 上一篇    

甲基丙烯酸酐明胶材料学特性及在皮肤组织工程应用与进展

吴  兴1,刘肇兴2,林欢欢2,刘  沙2,孙伟晶2,陈向军2
  

  1. 1内蒙古医科大学,内蒙古自治区呼和浩特市  010110;2解放军第二五三医院烧伤整形科,内蒙古自治区呼和浩特市  010010
  • 收稿日期:2017-09-28 出版日期:2018-01-18 发布日期:2018-01-18
  • 通讯作者: 陈向军,主任医师,硕士生导师,解放军第二五三医院烧伤整形科,内蒙古自治区呼和浩特市 010010
  • 作者简介:吴兴,男,1990年生,黑龙江省齐齐哈尔市人,汉族,内蒙古医科大学在读硕士,医师,主要从事皮肤软组织的损伤与修复研究。

Properties of gelatin methacryloyl and its application in the skin tissue engineering

Wu Xing1, Liu Zhao-xing2, Lin Huan-huan2, Liu Sha2, Sun Wei-jing2, Chen Xiang-jun2
  

  1. 1Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia Autonomous Region, China; 2Department of Burn and Plastic Surgery, the 253rd Hospital of PLA, Hohhot 010010, Inner Mongolia Autonomous Region, China
  • Received:2017-09-28 Online:2018-01-18 Published:2018-01-18
  • Contact: Chen Xiang-jun, Chief physician, Master’s supervisor, Department of Burn and Plastic Surgery, the 253rd Hospital of PLA, Hohhot 010010, Inner Mongolia Autonomous Region, China
  • About author:Wu Xing, Studying for master’s degree, Physician, Inner Mongolia Medical University, Hohhot 010110, Inner Mongolia Autonomous Region, China

摘要:

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文题释义:
甲基丙烯酸酐明胶:主要成分是廉价的变性胶原,拥有精氨酸-甘氨酸-天门冬氨酸基序(RGD)细胞结合基序和基质金属蛋白酶反应肽基序,由于RGD和基质金属蛋白酶反应肽基序的存在,甲基丙烯酸酐明胶支架可维持细胞生长增殖活力,调节细胞行为。甲基丙烯酸酐明胶可用于细胞的2D和3D培养,具有良好的细胞黏附性,能促进细胞的增殖、迁移和分化,可用来研究细胞外基质和细胞之间的相互作用。
甲基丙烯酸酐明胶在皮肤组织工程中的应用:表现出对组织无刺激、无毒无酶、无免疫原性、可减少炎症反应,GelMA的支架结构及RGD、基质金属蛋白酶反应肽基序、生长因子的存在将加速血管化,上皮组织增生加快创面愈合,降低了创面感染和瘢痕疙瘩形成的概率。
 
背景:甲基丙烯酸酐明胶具有合适的生物学特性、可调谐的理化特性、无细胞毒性的和非免疫原性,被广泛应用于组织工程领域。
目的:总结甲基丙烯酸酐明胶水凝胶材料的性能及应用于皮肤软组织损伤的最新研究进展。
方法:在PubMed和SciFinder中检索2007年1月至 2017年8月发表的与甲基丙烯酸酐明胶水凝胶相关的文献,检索关键词为:seed cell,skin regeneration,wound vascularization, gelatin,gelatin methacryloyl,scaffold material,wound healing,microenvironment,tissue construction,skin tissue engineering。
结果与结论:甲基丙烯酸酐明胶水凝胶与天然细胞外基质的特性非常相似,具有细胞黏附位点、基质金属蛋白酶反应肽基序列和可交联特性,表现出良好的组织亲和性,具有可调节的物理化学性质、一定的黏合性及可降解性,使其成为理想的细胞支架,允许各类细胞在其表面增殖和扩散。因此甲基丙烯酸酐明胶水凝胶在皮肤组织工程中有广阔的发展前景,可加速创面血管化和上皮组织再生,提高创面愈合速度,减少感染概率,提升患者生活质量,其可为烧伤创面治疗和战创伤救治提供一种高效、便携的凝胶敷料,还可用于填充外伤和溃疡等皮肤软组织缺损、术后美容切口的覆盖等。

关键词: 生物材料, GelMA, 明胶, 甲基丙烯酸酐明胶, 细胞支架, 皮肤组织工程, 水凝胶敷料, 创面愈合

Abstract:

BACKGROUND: Gelatin methacryloyl has been widely used in the field of tissue engineering, as it has suitable biological properties, tunable physical and chemical properties, non-cytotoxic and non-immunogenicity.
OBJECTIVE: To summarize the latest advances in the repair of skin and soft tissue damage using gelatin methacryl hydrogel materials. 
METHODS: PubMed and SciFinder were retrieved for articles concerning gelatin methacrylate hydrogels published from January 2007 to August 2017. The key words were “seed cell, skin regeneration, wound vascularization, gelatin, gelatin methacryloyl, scaffold material, wound healing, microenvironment, tissue construction, skin tissue engineering”.
RESULTS AND CONCLUSION: The gelatin methacryloyl hydrogel is very similar to the native extracellular matrix, and has a cell adhesion site, a matrix metalloproteinase-reactive peptide-based sequence and a cross-linkable property exhibiting good tissue affinity. The hydrogel has adjustable physical and chemical properties, a certain degree of adhesion and biodegradability, which make it an ideal cell scaffold, allowing all kinds of cells to proliferate and extend on its surface. Therefore, gelatin methacrylamide hydrogel has broad prospects in the skin tissue engineering, which can accelerate wound vascularization and epithelial tissue regeneration, improve wound healing rate, reduce the probability of infection, and improve the patient’s quality life. The gelatin methacrylamide hydrogen is proved to provide an efficient and portable gel dressing for burn wounds and war wounds, and it can also be used to fill skin and soft tissue defects such as trauma and ulcers, and cover cosmetic incisions.

Key words: Skin, Neovascularization, Physiologic, Gelatin, Tissue Engineering

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