中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (24): 4463-4466.doi: 10.3969/j.issn.1673-8225.2012.24.019

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

犬椎间盘经皮针刺退变模型的建立

彭 俊，徐建广   

  1. 上海交通大学附属第六人民医院，上海市 200233
  • 收稿日期:2011-10-22 修回日期:2011-11-14 出版日期:2012-06-10 发布日期:2013-11-05
  • 通讯作者: 徐建广，主任医师，上海市第六人民医院脊柱外科，上海市 200233 jianguangxu2004@yahoo.com.cn
  • 作者简介:彭俊★，男，1977年生，湖南省双峰市人，汉族，2010年上海交通大学毕业，硕士，主治医师，主要从事脊柱外科研究。 fengfooo@hotmail.com
  • 基金资助:

    上海市科委医学重点基金资助项目(09411953500)

Establishment of a dog disc degeneration model by percutaneous needle technique

Peng Jun, Xu Jian-guang   

  1. Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China
  • Received:2011-10-22 Revised:2011-11-14 Online:2012-06-10 Published:2013-11-05
  • Contact: Xu Jian-guang, Chief physician, Department of Spine Surgery, Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China
  • About author:Peng Jun★, Master, Attending physician, Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China

PDF

276

被引次数

7

摘要：

背景：普通针头穿刺纤维环是目前较为公认的理想椎间退变模型制作方法，但存在对动物损伤大，操作不简捷等缺点。
目的：尝试在C臂机透视下经皮穿刺比格犬腰椎椎间隙建立腰椎间盘退变动物模型。
方法：选用8只健康成年犬，行腰椎MRI后在C臂机下经皮穿刺椎间隙，损伤L5/6椎间盘。术后3，6个月行腰椎MRI后，取标本行免疫组织化学观察椎间盘退变情况。
结果与结论：经皮椎间盘穿刺后3，6个月，L5/6椎间盘均出现了明显退变，MRI显示T2值均出现明显降低，并且椎间盘大多出现不同程度椎间盘突出征象；L5/6穿刺节段髓核Ⅱ型胶原阳性细胞含量较L4/5髓核均明显减少(P < 0.05)。说明经皮穿刺损伤椎间盘建立犬椎间盘退变模型是一种简单有效、重复性好的建模方法。

关键词: 动物模型, 椎间盘退变, 犬, 微创, 组织构建实验造模

Abstract:

BACKGROUND: Annulus puncture using ordinary needles is recognized as an ideal construction method of intervertebral degeneration. However, there still exists certain disadvantages, such as great damage to animals, inconvenient to operate.
OBJECTIVE: To establish an animal model of lumbar intervertebral disc degeneration in Beagle dogs through percutaneous paracentesis under the fluoroscopic guidance of C-arm equipment.
METHODS: Eight healthy adult dogs were involved. Percutaneous paracentesis of intervertenral space was performed under the fluoroscopic guidance of C-arm equipment after lumbar MRI examination to damage the L5/6 intervertebral disc. Lumbar MRI examination was performed in the 3rd and 6th months after operation. Specimens were obtained for immunohistochemical examination to observe the disc degeneration.
RESULTS AND CONCLUSION: Significant degeneration was found in L5/6 intervertebral disc in 3 and 6 months after ercutaneous paracentesis; MRI examination showed that T2 values were significant lower, and signs of varying degrees of herniated disk was found in the intervertebral disc. The amount of type Ⅱ collagen-positive nucleus pulposus cells in the puncture segment of L5/6 was significantly lower than that in the segment of L4/5 (P < 0.05). It indicates that the construction method of percutaneous needle technique for dog disc degeneration model is easy, effective and reproducible.

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