中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (24): 4467-4470.doi: 10.3969/j.issn.1673-8225.2012.24.020

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

实验性牙髓炎模型兔的构建

毛小泉1，邢 璐2，谈 顺2，吴多荣3，徐 普1，黄 茜1   

  1. 中南大学湘雅医学院附属海口医院，1 海南省口腔医学中心，2病理科，3中心实验室，海南省海口市 570208
  • 收稿日期:2011-11-04 修回日期:2011-12-03 出版日期:2012-06-10 发布日期:2013-11-05
  • 通讯作者: 徐普，博士，教授，主任医师，中南大学湘雅医学院附属海口医院口腔中 心 ? 海南省口腔医学中心，海南省海口市 570208
  • 作者简介:毛小泉★，男，1966年生，江西省高安市人，汉族，2006年遵义医学院毕业，硕士，副主任医师，主要从事牙体牙髓研究。 horse.m@163.com
  • 基金资助:

    海南省自然科学基金资助项目(807091)

Construction of experimental pulpitis models in rabbits

Mao Xiao-quan1, Xing Lu2, Tan Shun2, Wu Duo-rong3, Xu Pu1, Huang Qian1   

  1. 1Hainan Provincial Stomatology Center, 2Department of Pathology, 3Center Laboratory, Affiliated Haikou Hospital Xiangya School of Medicine, Central South University, Haikou 570208, Hainan Province, China
  • Received:2011-11-04 Revised:2011-12-03 Online:2012-06-10 Published:2013-11-05
  • Contact: Xu Pu, Doctor, Professor, Chief physician, Hainan Provincial Stomatology Center, Affiliated Haikou Hospital Xiangya School of Medicine, Central South University, Haikou 570208, Hainan Province, China
  • About author:Mao Xiao-quan★, Master, Associate chief physician, Hainan Provincial Stomatology Center, Affiliated Haikou Hospital Xiangya School of Medicine, Central South University, Haikou 570208, Hainan Province, China horse.m@163.com

PDF

447

被引次数

4

摘要：

背景：以往牙髓炎动物模型的建立大多应用内毒素，不能完全反映临床牙髓炎的形成过程。
目的：观察应用变形链球菌诱导兔牙牙髓炎模型不同时间炎症特征。
方法：选用20只健康新西兰大白兔，随机将下颌前牙分为实验组和对照组；在兔两颗下颌前牙舌面钻孔，对照组一颗置入生理盐水，实验组另一颗置入变形链球菌，术后1，3，5，7 d各处死兔5只；观察牙髓炎炎症转归。
结果与结论：实验组术后1 d炎症反应较轻，经过3，5，7 d牙髓炎症反应逐渐加重，牙髓血管充血、渗出严重，牙髓组织出现坏死。对照组牙髓炎症随着时间的推移，血管扩张逐渐加重，偶有牙髓组织坏死。结果表明，应用变形链球菌诱导牙髓炎症形成过程更符合牙髓炎病理学特征。

关键词: 牙髓炎, 动物模型, 炎症, 兔, 组织学

Abstract:

BACKGROUND: Animal models of pulpitis have been established using endotoxin in the past; however, they cannot fully reflect the formation process of clinical pulpitis.
OBJECTIVE: To observe the inflammatory characteristics at different time points of rabbit models of pulpitis induced by Streptococcus mutans.
METHODS: The mandibular anterior teeth of 20 healthy New Zealand rabbits were divided into experiment group and control group. The lingual surfaces of the rabbit mandibular anterior teeth were drilled; normal saline was imported into the control tooth, while Streptococcus mutans was imported into the experiment tooth. Five rabbits were sacrificed on 1, 3, 5 and 7 days after operation, respectively; inflammation outcomes of pulpitis was observed.
RESULTS AND CONCLUSION: Inflammatory reaction in the experiment group was slight on 1 day after operation, and was aggravated gradually on 3, 5 and 7 days after operation, accompanied with vascular hyperemia in the pulp, severe effusion and necrosis of the pulp tissue. Inflammation in the control group was aggravated gradually as time went on; very few necrosis were found in the pulp tissues. These findings indicate that the forming process of pulpitis induced by Streptococcus mutans is more suitable to the pathological features of pulpitis.

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