中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (24): 4459-4462.doi: 10.3969/j.issn.1673-8225.2012.24.018

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

六月龄大鼠去卵巢后建立骨质疏松症模型的可行性

郭 峰，李正南，刘晋平，朱江涛   

  1. 忻州市人民医院骨一科，山西省忻州市 034000
  • 收稿日期:2011-12-31 修回日期:2012-03-21 出版日期:2012-06-10 发布日期:2013-11-05
  • 作者简介:郭峰★，男， 1974年生，山西省五寨县人，汉族，山西医科大学在职硕士，主治医师，主要从事小儿骨科、创伤及骨关节疾病的研究与治疗。 xzliyq@163.com lzn032200@163.com

Feasibility of establishing an animal model of osteoporosis in ovariectomized rats aged 6 months

Guo Feng, Li Zheng-nan, Liu Jin-ping, Zhu Jiang-tao   

  1. Department of Orthopedics, Xinzhou People’s Hospital, Xinzhou 034000, Shanxi Province, China
  • Received:2011-12-31 Revised:2012-03-21 Online:2012-06-10 Published:2013-11-05
  • About author:Guo Feng★, Studying for master’s degree, Attending physician, Department of Orthopedics, Xinzhou People’s Hospital, Xinzhou 034000, Shanxi Province, China xzliyq@163.com, lzn032200@163.com

PDF

351

被引次数

13

摘要：

背景：目前关于鼠龄对去势雌性大鼠建立骨质疏松模型影响的报道较少。
目的：验证6月龄大鼠去卵巢对构建骨质疏松症模型的可行性。
方法：6月龄雌性大鼠48只，分为2组，去卵巢组摘除双侧卵巢建立大鼠骨质疏松模型，假手术组不摘除卵巢，切除卵巢周围少量脂肪组织。建模后1，2，3个月测定大鼠体质量、子宫湿质量、碱性磷酸酶、抗酒石酸酸性磷酸酶、骨矿密度和骨矿含量等指标变化。
结果与结论：建模后1，2，3个月，去卵巢组比假手术组大鼠体质量明显增加(P < 0.05)，子宫湿质量较假手术组量明显下降(P < 0.05)。建模后1个月，去卵巢组大鼠血清碱性磷酸酶指标显著高于假手术组(P < 0.05)；建模后3个月，去卵巢组抗酒石酸酸性磷酸酶指标显著高于假手术组(P < 0.05)；碱性磷酸酶指标和抗酒石酸酸性磷酸酶指标随大鼠月龄增加有轻度增高趋势。建模后2，3个月去卵巢组骨矿密度显著低于假手术组(P < 0.05)。表明6月龄大鼠去卵巢可成功建立骨质疏松症模型。

关键词: 骨密度, 去卵巢, 骨质疏松症, 碱性磷酸酶, 抗酒石酸酸性磷酸酶

Abstract:

BACKGROUND: There are rare reports about establishing an animal model of osteoporosis in ovariectomized rats.
OBJECTIVE: To explore the feasibility to establish an animal model of osteoporosis in ovariectomized rats aged 6 months.
METHODS: Forty-eight female rats aged 6 months old were divided into sham operation group and ovariectomized group. Bilateral ovaries were removed in the ovariectomized group to establish osteoporosis models. A small amount of adipose tissue around the ovary was removed in the sham operation group. Body weight, wet weight of the uterine, alkaline phosphatase, tartrate-resistant acid phosphatase, bone mineral density and bone mineral content were measured at 1, 2 and 3 months after modeling.
RESULTS AND CONCLUSION: At 1, 2, 3 months after modeling, the ovariectomized rats had a significantly increase in the body weight as well as a decreased in wet weight of the uterine (P < 0.05). Serum alkaline phosphatase and tartrate-resistant acid phosphatase levels in the ovariectomized group were significantly higher than those in the sham operation group at 1 and 3 months, respectively (P < 0.05). Serum alkaline phosphatase and tartrate-resistant acid phosphatase levels had a slight increasing tendency with aging in rats. After 2 and 3 months, bone mineral density in the ovariectomized group was significantly lower than that in the sham operation group (P < 0.05). These findings indicate that the animal model of osteoporosis can be established successfully in ovariectomized rats aged 6 months.

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