中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4279-4283.doi: 10.3969/j.issn.1673-8225.2012.23.020

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

单核细胞趋化蛋白1与神经干细胞的体外迁移

董 岳1，康 波2，丁 鹏1，戴孝森1，王崇谦1，王伟民1，尚亚军1，王进昆1   

  1. 1昆明医学院第一附属医院神经外科，云南省昆明市 650000；
    2昆明市延安医院神经外科，云南省昆明市 650000
  • 收稿日期:2011-09-29 修回日期:2011-11-14 出版日期:2012-06-03 发布日期:2013-11-06
  • 通讯作者: 王进昆，教授，硕士生导师，昆明医学院第一附属医院神经外科，云南省昆明市 650000
  • 作者简介:董岳★，男，1984年生，贵州省铜仁市人，侗族，昆明医学院第一附属医院在读硕士，主要从事脑血管病基础与临床研究。Dongyue2005110@126.com 并列第一作者：康波，男，1980年生，山西省太原市人，汉族，医师。
  • 基金资助:

    2008年云南应用基础研究面上项目(2008ZC132M，0920033）：趋化因子及其受体在NSCs迁移中的作用的研究，MCP-1/CCR2通路在神经干细胞趋化迁移中作用的研究。

In vitro migration of monocyte chemoattractant protein 1 and neural stem cells

Dong Yue1, Kang Bo2, Ding Peng1, Dai Xiao-sen1, Wang Chong-qian1, Wang Wei-min1, Shang Ya-jun1, Wang Jin-kun1   

  1. 1Department of Neurosurgery, First Affiliated Hospital of Kunming Medical College, Kunming 650000, Yunnan Province, China;
    2Department of Neurosurgery, Yan’an Hospital of Kunming, Kunming 650000, Yunnan Province, China
  • Received:2011-09-29 Revised:2011-11-14 Online:2012-06-03 Published:2013-11-06
  • Contact: Wang Jin-kun, Professor, Master’s supervisor, Department of Neurosurgery, First Affiliated Hospital of Kunming Medical College, Kunming 650000, Yunnan Province, China
  • About author:Dong Yue★, Studying for master’s degree, Department of Neurosurgery, First Affiliated Hospital of Kunming Medical College, Kunming 650000, Yunnan Province, China Dongyue2005110@126.com

PDF

402

被引次数

12

摘要：

背景：研究表明趋化因子基质细胞衍生因子1及血管内皮生长因子参与神经干细胞的迁移。
目的：观察趋化因子单核细胞趋化蛋白1及其受体CCR2对神经干细胞体外迁移的作用。
方法：分离培养胎鼠海马组织神经干细胞，体外传代扩增及鉴定；通过细胞免疫荧光及RT-PCR检测其受体CCR2表达情况；通过琼脂糖下细胞迁移实验观察50，100，200，300，500 μg/L 单核细胞趋化蛋白1对神经干细胞的体外趋化作用。
结果与结论：细胞免疫荧光及RT-PCR检测证实胎鼠海马来源神经干细胞表达趋化因子受体CCR2；琼脂糖下细胞迁移实验显示，体外条件下单核细胞趋化蛋白1可趋化神经干细胞迁移，且趋化迁移作用随质量浓度增加而增强，抗CCR2多克隆抗体可对抗其趋化迁移作用。

关键词: 单核细胞趋化蛋白1, 神经干细胞, 趋化因子, 细胞迁移, CCR2

Abstract:

BACKGROUND: Studies have shown that the chemokine stromal cell derived factor-1 and vascular endothelial growth factor have the ability to make neural stem cells (NSCs) migration.
OBJECTIVE: To explore the effect of monocyte chemoattractant protein 1 (MCP-1) and its receptoron the migrati on of NSCs in vitro.
METHODS: Hippocampal NSCs from SD fetal rats were isolated and cultured; meanwhile they could be amplified and identified in vitro; The expression of CCR2 was detected with immunofluorescence and RT-PCR; Effect of MCP-1 with different concentrations (50, 100, 200, 300, 500 ng/mL) on NSCs in vitro migration was obsened under agarose.
RESULTS AND CONCLUSION: Immunofluorescence and RT-PCR results showed that NSCs from fetal rat hippocampal could express chemokine receptor CCR2; and the migration experiment under agarose showed that the MCP-1 with different concentrations could induce the migration of NSCs in vitro and with the higher the dose the better the migration. But the anti-CCR42 polyclonal antibodies could inhibit the migration of NSCs.

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