中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4232-4236.doi: 10.3969/j.issn.1673-8225.2012.23.011

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

兔脂肪干细胞体外诱导分化为胰岛β样细胞的可行性

杨 光1，赵占志2，朱新兵1，胡 松1   

  1. 辽河油田中心医院，1普外科， 2麻醉科，辽宁省盘锦市 124000
  • 收稿日期:2011-11-03 修回日期:2011-12-10 出版日期:2012-06-03 发布日期:2013-11-06
  • 作者简介:杨光★，男，1967年生，辽宁省盘锦市人，1993年中国医科大学毕业，硕士，副主任医师，主要从事肝胆疾病诊断与治疗方面的研究。 guangyang1967@qq.com

Differentiation of adipose-derived stem cells into islet β-like cells in vitro

Yang Guang1, Zhao Zhan-zhi2, Zhu Xin-bing1, Hu Song1   

  1. 1Department of General Surgery;
    2Department of Anesthesiology, the Central Hospital of Liaohe Oil Field, Panjin 124000, Liaoning Province, China
  • Received:2011-11-03 Revised:2011-12-10 Online:2012-06-03 Published:2013-11-06
  • About author:Yang Guang★, Master, Associate chief physician, Department of General Surgery, the Central Hospital of Liaohe Oil Field, Panjin 124000, Liaoning Province, China guangyang1967@qq.com

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摘要：

背景：胚胎干细胞、胰腺干细胞、肝卵圆细胞、小肠上皮干细胞和骨髓间充质干细胞均可以在体外诱导分化为胰岛β样细胞，那么脂肪干细胞是否也可以呢？
目的：探讨兔脂肪干细胞向胰岛β样细胞分化的可行性。
方法：以常规方法从兔脂肪抽吸物中分离、培养脂肪干细胞；传2代后，用高浓度葡萄糖(25 mmol/L)培养液DMEM以及碱性成纤维生长因子和尼克酰胺诱导兔脂肪干细胞向胰岛β样细胞分化。
结果与结论：第2代兔脂肪干细胞经过高糖诱导后，细胞形成细胞团；并且形成了双硫腙染色阳性的细胞团；免疫细胞化学表明诱导后细胞内的细胞胰岛素染色阳性。说明兔脂肪干细胞在体外可以定向诱导分化为胰岛β样细胞，这种胰岛β样细胞具有表达、储存胰岛素的功能。

关键词: 胰岛β样细胞, 脂肪干细胞, 体外分化, 碱性成纤维细胞生长因子, 尼克酰胺, 干细胞

Abstract:

BACKGROUND: Islet β-like cells can be induce-differentiated by embryonic stem cells, pancreatic stem cells, hepatic oval cells, intestinal stem cells and bone marrow mesenchymal stem cells in vivo. Then whether adipose-derived stem cells can be differentiate into islet β-like cells remains pooly understod?
OBJECTIVE: To investigate the feasibility of adipose-derived stem cells to differentiate into islet β-like cells.
METHODS: Adipose-derived stem cells were isolated and cultured from rabbit liposuction, and after two generations passage, the adipose-derived stem cells were differentiated into islet β-like cells induced by high glucose, basic fibroblast growth factorand nicotamine in culture medium.
RESULTS AND CONCLUSION: After the passage 2 adipose-derived stem cells were induced by high glucose, the adipose-derived stem cells formed cell clusters and dithizone-stained cell clusters in flask were observed. Immunocytochemistry also confirmed that these cell clusters were positive for insulin. Adipose-derived stem cells can differentiate into islet β-cells in vitro. These islet β-cells can synthesize and store insulin.

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