中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4227-4231.doi: 10.3969/j.issn.1673-8225.2012.23.010

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

体外诱导脂肪基质干细胞向多巴胺能神经元的分化

高 华1，2，姚亚妮1，王玉玲1，杨新玲1   

  1. 1新疆医科大学第一附属医院神经医学中心，新疆维吾尔自治区乌鲁木齐市 830054；
    2新疆医科大学第五附属医院神经内科，新疆维吾尔自治区乌鲁木齐市 830011
  • 收稿日期:2011-09-24 修回日期:2011-10-27 出版日期:2012-06-03 发布日期:2013-11-06
  • 通讯作者: 杨新玲，博士，主任医师，教授，新疆医科大学第一附属医院神经医学中心，新疆维吾尔自治区乌鲁木齐市 830054 poplar862@sohu.com
  • 作者简介:高华★，女，1979年生，新疆维吾尔自治区伊犁巩留县人，汉族，2011年新疆医科大学第一附属医院毕业，硕士，医师，主要从事帕金森病的研究。 gaohuasnow@163.com

In vitro induced differentiation of adipose stem cells into dopaminergic neurons

Gao Hua1, 2, Yao Ya-ni1, Wang Yu-ling1, Yang Xin-ling1   

  1. 1Center of Neurological Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China;
    2Department of Neurology, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-09-24 Revised:2011-10-27 Online:2012-06-03 Published:2013-11-06
  • Contact: Yang Xin-ling, M.D., Chief physician, Professor, Center of Neurological Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China poplar862@sohu.com
  • About author:Gao Hua★, Master, Physician, Center of Neurological Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Department of Neurology, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China gaohuasnow@163.com

PDF

315

被引次数

4

摘要：

背景：鉴于脂肪干细胞具有来源丰富、取材简便、易分离纯化和扩增、可进行自体移植并有多项分化潜能等特点，有学者认为其可作为治疗帕金森病的种子细胞来源。
目的：观察大鼠脂肪基质干细胞体外向多巴胺能神经元诱导分化及分泌功能。
方法：利用胶原酶消化法获得SD 大鼠脂肪干细胞，体外培养至第 3代时用碱性成纤维细胞生长因子、表皮生长因子和抗坏血酸联合诱导作为实验组，以单纯加入体积分数5%血清为对照组。
结果与结论：实验组诱导3 d后，免疫荧光法和免疫组织化学法均可检测到诱导后的细胞表达酪氨酸羟化酶。实验组分化出多巴胺能神经元比例显著高于对照组(P < 0.01)。ELISA法检测实验组连续诱导3 d上清中有多巴胺分泌，诱导的第一二天中多巴胺浓度呈上升趋势，第3天有所下降。说明脂肪干细胞具有向多巴胺能神经元分化的能力。

关键词: 多巴胺能神经元, 脂肪基质干细胞, SD大鼠, 神经营养因子, 抗坏血酸, 干细胞

Abstract:

BACKGROUND: Adipose stem cells are considered as the seed cells for the treatment of Parkinson’s disease due to their characteristics such as rich sources, simple drawing, easy to purify and amplify, autologous transplantation and multipotential differentiation.
OBJECTIVE: To observe the differentiation of rat adipose stem cells into dopaminergic neurons and their secreted function.
METHODS: The adipose stem cells of Sprague -Dawley rats were obtained by collagenase digestion. In the experimental group, the cells were differentiated into dopaminergic neurons co-induced by basic fibroblast growth factor, epidermal growth factor and ascorbic acid after cultured to the third passage in vitro. In the control group, the cells were simply added with 5% serum.
RESULTS AND CONCLUSION: Tyrosine hydroxylase expression in the experimental group was examined by immunofluorescence and immunohistochemisty after induced for 3 days. The proportion of dopaminergic neurons in experimental group was significantly higher than that in the control group (P < 0.01). The level of dopaminergic neurons in cell supernatant was detected by ELISA assay after successively induced for 3 days; the concentration of dopaminergic neurons was increased at the first and second days after induction, and decreased at the third day. The results showed that the adipose stem cells could be differentiated into dopaminergic neurons.

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