中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4213-4216.doi: 10.3969/j.issn.1673-8225.2012.23.007

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

脐血单个核细胞与骨髓间充质干细胞滋养层的共培养

王 雷1，宋 洁2，匡 弢2   

  1. 1青岛市立医院血液科，山东省青岛市 266071；
    2青岛大学医学院附属医院特需保健科，山东省青岛市 266003
  • 收稿日期:2011-11-23 修回日期:2012-03-15 出版日期:2012-06-03 发布日期:2013-11-06
  • 通讯作者: 匡弢，副主任医师，青岛大学医学院附属医院特需保健科，山东省青岛市 266003 kuangtk@126.com
  • 作者简介:王雷，男，1962年生，山东省昌邑市人，汉族，1987年山东大学毕业，副主任医师，主要从事造血干细胞移植治疗恶性血液病及疑难血液病的研究。 qingdaowanglei108@sina.com

Co-culture of umbilical cord blood-derived monocytes and bone marrow mesenchymal stem cells

Wang Lei1, Song Jie2, Kuang Tao2   

  1. 1Department of Hematology, Qingdao Municipal Hospital, Qingdao 266071, Shandong Province, China;
    2Department of Special Health Care, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China
  • Received:2011-11-23 Revised:2012-03-15 Online:2012-06-03 Published:2013-11-06
  • Contact: Kuang Tao, Associate chief physician, Department of Special Health Care, Affiliated Hospital of Medical College of Qingdao University, Qingdao 266003, Shandong Province, China kuangtk@126.com
  • About author:Wang Lei, Associate chief physician, Department of Hematology, Qingdao Municipal Hospital, Qingdao 266071, Shandong Province, China qingdaowanglei108@sina.com

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摘要：

背景：骨髓间充质干细胞具有维持骨髓正常造血功能，在造血调控中发挥重要的作用。
目的：观察骨髓间充质干细胞的生物学性状和多向分化能力，并检测其在体外支持造血的能力。
方法：利用密度梯度培养法分离骨髓单个核细胞进行培养，流式细胞仪检测骨髓间充质干细胞表型；接种脐血单个核细胞于骨髓间充质干细胞滋养层培养板上共培养，观察粒-单系祖细胞集落变化。
结果与结论：骨髓间充质干细胞呈典型的成纤维样细胞形态，强表达CD44，CD29，不表达CD34和CD106。可促进脐血单个核细胞扩增并形成造血祖细胞集落。提示骨髓间充质干细具有造血支持作用。

关键词: 脐血, 造血, 骨髓间充质干细胞, 造血祖细胞集落, 共培养, 干细胞

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells support hematopoiesis in vivo and in vitro and play an important role in regulation of hematopoiesis.
OBJECTIVE: To investigate the biological characteristics and multi-directional differentiation capability of bone marrow mesenchymal stem cells and their ability to support hematopoiesis in vivo and in vitro.
METHODS: Bone marrow monocytes were isolated by density gradient culture method. Bone marrow mesenchymal stem cell phenotype was detected by flow cytometry. Umbilical cord monocytes were inoculated onto bone marrow mesenchymal stem cell trophoblast for co-culture. Granulocyte-monocyte colony forming units were counted.
RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells showed typical fibroblast-like cell morphology and strongly expressed CD44 and CD29, but they were negative for CD34 and CD106. Bone marrow mesenchymal stem cells could promote the proliferation of umbilical cord monocytes to form hematopoietic progenitor colonies. These findings suggest that bone marrow mesenchymal stem cells support hematopoiesis.

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