中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4187-4192.doi: 10.3969/j.issn.1673-8225.2012.23.002

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞移植促进脑缺血性损伤大鼠神经细胞黏附分子的表达

彭 玉，张其梅，游 辉，张 颖，庄伟华，张 强，周敬华   

  1. 三峡大学第一临床医学院&宜昌市中心人民医院，湖北省宜昌市 443003
  • 收稿日期:2012-01-01 修回日期:2012-03-28 出版日期:2012-06-03 发布日期:2013-11-06
  • 通讯作者: 张其梅，主任医师，教授，三峡大学第一临床医学院&宜昌市中心人民医院，湖北省宜昌市 443003
  • 作者简介:彭玉★，女，1974年生，湖北省宜都市人，汉族，2007年武汉大学医学院毕业，硕士，副主任医师，主要从事脑血管病的基础与临床研究。 ycpengyu@126.com 游 辉，1971年生，湖北省麻城市人，汉族，1997年武汉大学医学院毕业，副主任医师，主要从事炎症性肠病研究

Bone marrow-derived mesenchymal stem cell transplantation increased expression of neural cell adhesion molecules in a rat model of ischemic brain injury

Peng Yu, Zhang Qi-mei, You Hui, Zhang Ying, Zhuang Wei-hua, Zhang Qiang, Zhou Jing-hua   

  1. First Clinical College of China Three Gorges University & Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China
  • Received:2012-01-01 Revised:2012-03-28 Online:2012-06-03 Published:2013-11-06
  • Contact: Zhang Qi-mei, Professor, Chief physician, First Clinical College of China Three Gorges University & Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China
  • About author:Peng Yu, Master, Associate chief physician, First Clinical College of China Three Gorges University & Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China ycpengyu@126.com You Hui, Associate chief physician, First Clinical College of China Three Gorges University & Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China

PDF

305

被引次数

2

摘要：

背景：骨髓间充质干细胞移植能促进神经功能修复，其作用机制可能与上调神经细胞黏附分子有关。
目的：观察骨髓间充质干细胞移植对急性脑缺血损伤大鼠神经细胞黏附分子表达的影响。
方法：将SD大鼠按随机数字表法分为假手术组和模型组。模型组大鼠采用线栓法制作大脑中动脉阻塞模型，再随机分为细胞移植组(左侧侧脑室移植同种异体骨髓间充质干细胞5×105)和对照组(移植磷酸盐缓冲液)。假手术组不闭塞大脑中动脉也不移植。
结果与结论：移植后7，14 d，细胞移植组与对照组梗死灶周围神经细胞黏附分子表达高于假手术组(P < 0.01)，且细胞移植组高于对照组(P < 0.05)。移植后14 d，细胞移植组神经功能缺损评分明显低于对照组(P < 0.05)。说明骨髓间充质干细胞移植后通过上调局灶性脑缺血大鼠脑梗死灶周神经细胞黏附分子表达促进神经功能的恢复。

关键词: 脑缺血, 骨髓间充质干细胞, 细胞移植, 疾病模型, 大鼠, 干细胞

Abstract:

BACKGROUND: Studies have demonstrated that transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves neurofunctional recovery and the underlying mechanism may be related to upregulated expression of neural cell adhesion molecules.
OBJECTIVE: To investigate the effects of BMSC transplantation on the expression of neural cell adhesion molecule after ischemic brain injury in rats.
METHODS: Sprague-Dawley rats were randomly divided into a sham-operated group and a middle cerebral artery occlusion (MCAO) group. A permanent focal cerebral ischemia model was established using modified Longa’s method. The MCAO groups were randomly subdivided into a BMSC group (transplantation of 5×105 allogenic BMSCs via the left lateral ventricle) and a control group (phosphate buffered saline injection). The sham-operated group received neither MCAO nor BMSC transplantation.
RESULTS AND CONCLUSION: At 7 and 14 days following transplantation, neural cell adhesion molecule expression surrounding the infarct foci was significantly higher in the BMSC group and control group (P < 0.01), and the expression in the BMSC group was significantly higher than in the control group (P < 0.05). At 14 days following transplantation, neurological severity score in the BMSC group was significantly lower than in the control group (P < 0.05). BMSC transplantation promoted neurofunctional recovery by upregulating neural cell adhesion molecule expression in the marginal zone of cerebral infarct focus of a rat model of focal cerebral ischemia.

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