中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (21): 3895-3898.doi: 10.3969/j.issn.1673-8225.2012.21.022

• 药物控释材料 drug delivery materials • 上一篇    下一篇

可注射藻酸盐支架与人骨髓基质干细胞的体外复合培养*☆

汪群力1，龚继承1，邹重文1，杨柏林1，罗伟国1，金  丹2，魏宽海2   

  1. 1解放军第一八七中心医院骨二科，海南省海口市 571159；2南方医科大学附属南方医院创伤骨科，广东省广州市 510515
  • 收稿日期:2012-01-09 修回日期:2012-02-21 出版日期:2012-05-20 发布日期:2012-05-20
  • 作者简介:汪群力☆，男，1973年生，湖北省黄冈市团风县人，汉族，2005年解放军第一军医大学毕业，博士，副主任医师，主要从事关节外科、关节镜、组织工程方面的研究。Wangqunli2005@yahoo.com.cn
  • 基金资助:

    海南省自然科学基金项目(808248)。

In vitro co-culture of injectable alginate scaffolds and human bone marrow stromal stem cells

Wang Qun-li1, Gong Ji-cheng1, Zou Zhong-wen1, Yang Bo-lin1, Luo Wei-guo1, Jin Dan2, Wei Kuan-hai2   

  1. 1Second Department of Orthopedics, the 187 Central Hospital of Chinese PLA, Haikou  571159, Hainan Province, China; 2Department of TraumaticOrthopedics, Nanfang Hospital of Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Received:2012-01-09 Revised:2012-02-21 Online:2012-05-20 Published:2012-05-20
  • About author:Wang Qun-li☆, Doctor, Associate chief physician, Second Department of Orthopedics, the 187 Central Hospital of Chinese PLA, Haikou 571159, Hainan Province, China Wangqunli2005@yahoo.com.cn
  • Supported by:

    the Natural Science Foundation of Hainan Province, No. 808248*

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摘要：

背景：目前可注射组织工程骨的研究主要限于动物实验，若人骨髓基质干细胞与藻酸盐生物相容性良好，可注射组织工程骨将是极具前途的临床治疗手段。
目的：体外观察人骨髓基质干细胞与可注射支架藻酸钙凝胶的生物相容性。
方法：实验组将第2代人骨髓基质干细胞与藻酸钙凝胶复合培养，对照组单纯接种骨髓基质干细胞。倒置相差显微镜、扫描电镜观察各组细胞形态及增殖情况，MTT法半定量检测细胞增殖情况。
结果与结论：倒置显微镜下见实验组细胞生长良好，与对照组无明显差异。扫描电镜见骨髓基质干细胞在藻酸钙表面贴附、增殖良好，第6天时细胞已跨越微孔表面或向孔内生长。MTT法显示与对照组相比，实验组细胞增殖能力不受影响。结果初步表明藻酸钙与人骨髓基质干细胞体外生物相容性较好。
 

关键词: 藻酸钙凝胶, 可注射, 人骨髓基质干细胞, 生物材料, 生物相容性

Abstract:

BACKGROUND: Injectable tissue-engineered bone is limited to animal experiments. If the human bone marrow stromal stem cells have a good biocompatibility with alginate, the injectable tissue engineered bone will be a very promising clinical treatment.
OBJECTIVE: To study the biocompatibility of human bone marrow stromal stem cells and injectable scaffold of calcium alginate gels in vitro.
METHODS: The second passage human bone marrow stromal stem cells were co-cultured with calcium alginate gels. Bone marrow stromal stem cells cultured with no material served as controls. The morphology and proliferation of the cells were observed by inverted phase contrast microscope and scanning electron microscope. 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay was used to semi-quantitatively evaluate the cell proliferation.
RESULTS AND CONCLUSION: The human bone marrow stromal stem cells co-cultured with calcium alginate gels grew well under inverted phase contrast microscope, and no significant difference was found between calcium alginate gels group and control group. Under scanning electron microscope, human bone marrow stromal stem cells adhered well to the calcium alginate gels and proliferated obviously. MTT results showed that the cell number had no significant difference between the two groups. It is suggested that calcium alginate gels have good biocompatibility with human bone marrow stromal stem cells.

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