中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (21): 3843-3846.doi: 10.3969/j.issn.1673-8225.2012.21.010

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

改性纳米羟基磷灰石/聚乳酸-聚羟乙酸复合骨髓基质干细胞修复兔桡骨缺损*★

谭羽莹1，邵  英1，李玉新2，徐  洋3，李  春1，金洪娟1，张  舵1   

  1. 1吉林大学第一医院整形美容外科，吉林省长春市  130021；2东北师范大学药物基因和蛋白筛选国家工程实验室，吉林省长春市 130024；3厦门大学附属第一医院思明分院 整形美容外科，福建省厦门市 361002
  • 收稿日期:2011-10-09 修回日期:2011-11-04 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 张舵，博士后，教授，吉林大学第一医院整形美容外科，吉林省长春市 130021 zhangduo2002@yahoo.com
  • 作者简介:谭羽莹★，女，1985年生，吉林省长春市人，汉族，吉林大学第一医院毕业，硕士，医师，主要从事整形美容外科及组织工程方面的研究。tyy_316@ 163.com
  • 基金资助:

    吉林大学科学前沿与交叉学科创新项目(200903068)。

Modified nano-hydroxyapatite/poly (lactide-glycolide acid) combined with bone marrow mesenchymal stem cells for repair of rabbit radial bone defects

Tan Yu-ying1, Shao Ying1, Li Yu-xin2, Xu Yang3, Li Chun1, Jin Hong-juan1, Zhang Duo1   

  1. 1Department of Plastic and Reconstructive Surgery, First Hospital of Jilin University, Changchun  130021, Jilin Province, China; 2National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun  130024, Jilin Province, China; 3Department of Plastic and Reconstructive Surgery, Siming Branch of First Affiliated Hospital, Xiamen University, Xiamen  361002, Fujian Province, China
  • Received:2011-10-09 Revised:2011-11-04 Online:2012-05-20 Published:2012-05-20
  • Contact: Zhang Duo, Doctor, Professor, Department of Plastic and Reconstructive Surgery, First Hospital of Jilin University, Changchun 130021, Jilin Province, China zhangduo2002@yahoo.com
  • About author:Tan Yu-ying★, Master, Physician, Department of Plastic and Reconstructive Surgery, First Hospital of Jilin University, Changchun 130021, Jilin Province, China tyy_316@163.com
  • Supported by:

    Science Frontiers and Cross- Disciplinary Innovation Projects of Jilin University, No. 200903068*

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摘要：

背景：前期试验证实骨髓基质干细胞能够在改性纳米羟基磷灰石/聚乳酸-聚羟乙酸材料表面黏附、增殖，该材料具有良好的生物安全性。
目的：观察骨髓基质干细胞与改性纳米羟基磷灰石/聚乳酸-聚羟乙酸材料复合修复兔桡骨缺损的效果。
方法：建立兔15 mm桡骨缺损模型，随机分为3组：空白对照组不进行任何处理，实验组植入改性纳米羟基磷灰石/聚乳酸-聚羟乙酸+骨髓基质干细胞组织工程化骨，对照组植入单纯改性纳米羟基磷灰石/聚乳酸-聚羟乙酸支架材料。
结果与结论：①X射线评价：术后1~12周，实验组骨缺损修复程度及速度明显优于空白对照组与对照组(P < 0.05)。②组织学检测：实验组术后4周即可观察到新生骨和纤维组织长入材料空隙，局部形成陷窝结构；8周时新生骨组织增多，部分可观察到成熟的骨小梁结构；12周时可见大量成熟骨细胞，骨小梁排列紧密，移植材料逐步被新生骨取代，与正常骨组织形态基本一致，且骨小梁出现时间早于空白对照组与对照组。说明骨髓基质干细胞复合改性纳米羟基磷灰石/聚乳酸-聚羟乙酸构建的组织工程化骨能够促进骨缺损处新骨的生成，较单纯支架材料具有明显优势。

关键词: 纳米羟基磷灰石, 聚乳酸-聚羟乙酸, 组织工程化骨, 骨髓基质干细胞, 骨缺损, 骨修复

Abstract:

BACKGROUND: Practical tests showed that the bone marrow mesenchymal stem cells (BMSCs) could proliferate and adhere on the surface of modifiednano-hydroxyapatite/poly (lactide-glycolide acid) (PLGA/g-HA) that demonstrated that the PLGA/g-HA had a good biocompatibility.
OBJECTIVE: To observe the effect of BMSCs combined with modified PLGA/g-HA on repair of rabbit radial bone defect.
METHODS: Model of 15 mm rabbit radial bone defect was established. The rabbits were randomly divided into three groups. The blank group was without any treatment, the experimental group was implanted with modified PLGA/g-HA + BMSCs, and the control group was treated with modified PLGA/g-HA.
RESULTS AND CONCLUSION: ①X-ray evaluation: The extent and speed of repair in the experimental group were obviously increased compared with the blank group and control group after 1-12 weeks of operation (P < 0.05). ②Histological observation: At 4 weeks, new formed bone could be seen, fibrous tissue extended into the space of scaffold, and bone lacuna was partially formed in the experimental group; At 8 weeks, new formed bone was increased and mature bone trabecula could be seen in the experimental group; Furthermore, at 12 weeks after operation, a great amount of mature osteoblasts could be seen, trabecular bone tightly packed, and the implants was gradually replaced by normal bone tissues in the experimental group. The experimental group showed its bone trabecula earlier than the blank group and control group. Tissue-engineered bone tissues constructed by BMSCs combined with modified PLGA/g-HA can promote the new bone formation of defect. The BMSCs combined with modified PLGA/g-HA are superior to the simple scaffolds.
 

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