中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (21): 3838-3842.doi: 10.3969/j.issn.1673-8225.2012.21.009

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

新型人工生物活性接骨板治疗犬股骨骨折*★◆

徐小平，倪卫东，高仕长，蒲  超，黄伟弘   

  1. 重庆医科大学附属第一医院骨科，重庆市神经病学重点实验室，重庆市  400016
  • 收稿日期:2011-12-10 修回日期:2012-03-09 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 倪卫东，主任医师，重庆医科大学附属第一医院骨科，重庆市 400016 niweidong18@163.com
  • 作者简介:徐小平★，男，1985年生，四川省资阳市人，汉族，重庆医科大学在读硕士，主要从事骨折愈合研究。Xuxiaoping020@sina.com
  • 基金资助:

    重庆市卫生局医学科研计划项目(2008-2-22)。

LIM mineralization protein 1 gene transfected bone marrow mesenchymal stem cells combined with nano-hydroxyapatite/polyamide 66 plate in the treatment of canine femoral fractures 

Xu Xiao-ping, Ni Wei-dong, Gao Shi-chang, Pu Chao, Huang Wei-hong   

  1. Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing  400016, China
  • Received:2011-12-10 Revised:2012-03-09 Online:2012-05-20 Published:2012-05-20
  • Contact: Ni Wei-dong, Chief physician, Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing 400016, China niweidong18@163.com
  • About author:Xu Xiao-ping★, Studying for master’s degree, Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing 400016, China Xuxiaoping020@ sina.com
  • Supported by:

     the Medical Science and Technology Research Program of Chongqing Health Bureau, No. 2008-2-22*

PDF

356

被引次数

3

摘要：

背景：研究表明纳米羟基磷灰石/聚酰胺66是一种具有良好的生物相容性、生物安全性、生物活性和骨传导能力的高强度高韧性复合材料。
目的：分析转染LIM矿化蛋白1基因的骨髓间充质干细胞复合纳米羟基磷灰石/聚酰胺66接骨板治疗犬股骨骨折的可行性和效果。
方法：收集犬骨髓间充质干细胞，经LIM矿化蛋白1转染后与纳米羟基磷灰石/聚酰胺66接骨板复合制备新型生物活性接骨板。48只杂交犬建立右侧股骨中段横形骨折模型，分别用转染/未转染LIM矿化蛋白1基因的骨髓间充质干细胞复合纳米羟基磷灰石/聚酰胺66接骨板、单纯纳米羟基磷灰石/聚酰胺66接骨板和钢板螺钉进行修复。
结果与结论：修复后8周及修复后12周转染组内固定失败率均低于未转染组和单纯接骨板组(P < 0.05)，与钢板螺钉修复比较差异无显著性意义(P > 0.05)。转染组骨折愈合时间明显短于其他组。修复后12周转染组接骨板与犬股骨外侧皮质完全融合，骨干间有明显新骨生成。未转染组和单纯接骨板组未见或少量骨组织生成。说明新型生物活性接骨板能促进骨折愈合并与自体骨融合，不需要二次手术取出，但该新型接骨板的强度有一定的局限性，用于犬股骨骨折的治疗时必须辅加外固定。
 

关键词: 纳米羟基磷灰石/聚酰胺66, 腺病毒, 骨折, 犬, LIM矿化蛋白1

Abstract:

BACKGROUND: Studies have shown that nano-hydroxyapatite/polyamide 66 (n-HA/PA66) is a kind of high-strength and high-toughness composite material with excellent biocompatibility, biological safety, biological activity and bone conduction ability.
OBJECTIVE: To investigate the effect and feasibility of n-HA /PA66 plate combined with bone marrow mesenchymal cells (BMSCs) transfected with human LIM mineralization protein 1 (LMP-1) gene for treatment of canine femoral fractures.
METHODS: Canine BMSCs were isolated and cultured with density gradient centifugation method and adhesive culture method. The third-generation BMSCs were transfected with Adv-hLMP-1, and then combined with n-HA /PA66 plate. The dog models of femoral fractures were established in 48 dogs and were divided into four groups: Ad-LMP-1 transfection group, non-transfected group, n-HA/PA66 alone group and plate and screw groups.
RESULTS AND CONCLUSION: The failure rate in the transfection group was lower than that in non-transfected group and n-HA/ PA66 plate group at 8 or 12 weeks postoperatively (P < 0.05), and had no statistical difference compared with plate and screw group (P > 0.05). The healing time of the transfection group was shorter than that of the other three groups. The plate in the transfection group was completely fused with canine femoral lateral cortex after 12 weeks of operation, and there was obvious bone formation between material and bone. The plate and canine lateral femoral cortex were only partially fused in the non-transfected group and n-HA/PA66 plate group, and there was no or a small amount of bone tissue formation between material and bone. The treatment of canine femoral fractures with n-HA/PA66 plate combined with BMSCs transfected with LMP-1 gene can get good fixation effects, promote the fracture healing and fusion with autogenous bone, without the need for a second operation to take out, but the strength of materials have some limitations, which must be combined with external fixation in the treatment of canine femoral fractures.

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