中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (21): 3819-3822.doi: 10.3969/j.issn.1673-8225.2012.21.005

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

自体红骨髓构建非细胞型组织工程化骨修复骨缺损**★

黄文良1，邓  江1，贾东林2，阮世强1，苑  成1   

  1. 1遵义医学院第三附属医院，贵州省遵义市  563002；2山东省兖州市第一人民医院，山东省兖州市  272116
  • 收稿日期:2012-01-17 修回日期:2012-03-13 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 邓江，主任医师，硕士生导师，遵义医学院第三附属医院骨科一病区，贵州省遵义市 563002 DJ30666@126.com
  • 作者简介:黄文良★，男，1981年生，广西壮族自治区南宁市人，2010年遵义医学院毕业，硕士，医师，主要从事创伤骨科研究。
  • 基金资助:

    遵义市科技基金项目(遵市科合社字[2007] 15号)；贵州省社会攻关项目(黔科合SY字[2010]3101号)。

Repairing bone defects by non-cell based tissue engineered complex constructed by autologous red bone marrow

Huang Wen-liang1, Deng Jiang1, Jia Dong-lin2, Ruan Shi-qiang1, Yuan Cheng1   

  1. 1Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China; 2Department of Orthopedics, First People’s Hospital of Yanzhou City, Yanzhou  272116, Shandong Province, China
  • Received:2012-01-17 Revised:2012-03-13 Online:2012-05-20 Published:2012-05-20
  • Contact: Deng Jiang, Chief physician, Master’s supervisor, Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China DJ30666@126.com
  • About author:Huang Wen-liang★, Master, Physician, Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical College, Zunyi 563000, Guizhou Province, China
  • Supported by:

    the Science and Technology Foundation of Zunyi City, No. [2007]15*; the Social Tackle Key Program of Guizhou Province, No. [2010]3101*

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摘要：

背景：细胞型组织工程化骨修复骨缺损的效果良好，但存在操作复杂，容易污染及花费时间长等不足，不利于临床应用。
目的：观察自体红骨髓构建的非细胞型组织工程化骨修复大段骨缺损的成骨效果。
方法：在27只家兔一侧桡骨制作2 cm大段骨缺损模型后，随机分3组，分别植入自体红骨髓+重组人胰岛素样生长因子1/珊瑚羟基磷灰石非细胞型组织工程化骨、自体红骨髓/珊瑚羟基磷灰石复合人工骨材料、珊瑚羟基磷灰石/重组人胰岛素样生长因子1复合人工骨材料。
结果与结论：植入后4，8，12周，从植入物内部血管化程度、骨小梁数量、成熟骨结构形成及植入物的降解分析比较，自体红骨髓+重组人胰岛素样生长因子1/珊瑚羟基磷灰石非细胞型组织工程化骨组成骨能力及材料降解明显优于其他两组  (P < 0.05)。表明自体红骨髓构建的非细胞型组织工程化骨具有较高的成骨活性，修复大段骨缺损有显著效果。
关键词：自体红骨髓；组织工程骨；重组人胰岛素样生长因子1；珊瑚羟基磷灰石；骨缺损；骨移植；扫描电镜
doi:10.3969/j.issn.1673-8225.2012.21.005

关键词: 自体红骨髓, 组织工程骨, 重组人胰岛素样生长因子1, 珊瑚羟基磷灰石, 骨缺损, 骨移植, 扫描电镜

Abstract:

BACKGROUND: Cell based tissue engineered bone is successful in the repair of bone defects, but it is a complex operation, easy to be contaminated and takes a long time, and not conducive to clinical application.
OBJECTIVE: To investigate the efficacy of repairing large-segment bone defects by non-cell based tissue engineered complex constructed by autologous red bone marrow.
METHODS: Twenty-seven rabbits were used to prepare 2 cm unilateral radial large-segment bone defect models, and then randomly divided into group A, group B and group C. Group A was implanted with autologous red bone marrow to construct tissue engineered bone (autologous red bone marrow+recombinant human insulin-like growth factor-1/coralline hydroxyapatite); group B was implanted with autologous red bone marrow/coralline hydroxyapatite artificial bone material; group C was implanted with coralline hydroxyapatite/recombinant human insulin-like growth factor-1 artificial bone material.
RESULTS AND CONCLUSION: Group A was superior to the other two group in terms of osteogenic potential and material degradation by comparison of degree of bone formation, bone trabecular number, the structure formation of mature bone and implant degradation at 4, 8, 12 weeks after implantation (P < 0.05). Non-cell based tissue engineered bone constructed by autologous red bone marrow has better osteogenic activity, and effective to repair large-segment bone defects.

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