中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2709-2714.doi: 10.3969/j.issn.1673-8225.2012.15.011

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

热休克蛋白90和凋亡抑制蛋白Livin在病理性瘢痕中的表达★

王大伟1, 2，王喜梅1，刘林嶓1，王琪影1，彭  倩1，张  娟1, 2，孙玉峰1, 2，张琼阁1, 2   

  1. 1郑州大学第一附属医院整形外科，河南省郑州市  450052；2河南省高等学校临床医学重点学科开放实验室，河南省郑州市  450052
  • 收稿日期:2011-10-10 修回日期:2011-11-24 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 王喜梅，博士，教授，主任医师，硕士生导师，郑州大学第一附属医院整形外科，河南省郑州市 450052 wangximei228@gmail.com
  • 作者简介:王大伟★，男，1982年生，河南省焦作市人，汉族，郑州大学第一附属医院整形外科在读硕士，主要从事病理性瘢痕的基础与临床研究。wdw901@yahoo.com.cn

Expression of heat shock protein 90 and inhibitor of apoptosis protein Livin in pathologic scars

Wang Da-wei1, 2, Wang Xi-mei1, Liu Lin-bo1, Wang Qi-ying1, Peng Qian1, Zhang Juan1, 2, Sun Yu-feng1, 2, Zhang Qiong-ge1, 2   

  1. 1Department of Plastic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou  450052, Henan Province, China; 2Key-Disciplines Laboratory Clinical-Medicine Henan, Zhengzhou  450052, Henan Province, China
  • Received:2011-10-10 Revised:2011-11-24 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Wang Xi-mei, Doctor, Professor, Chief physician, Master’s supervisor, Department of Plastic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
  • About author:Wang Da-wei★, Studying for master’s degree, Department of Plastic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; Key-disciplines Laboratory Clinical-Medicine Henan, Zhengzhou 450052, Henan Province, China wdw901@yahoo.com.cn

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摘要：

背景：近年来研究表明热休克蛋白90和凋亡抑制蛋白Livin在细胞的凋亡过程中扮演着非常重要的角色，而两者在病理性瘢痕形成过程中的作用研究至今鲜有报道。
目的：从基因和蛋白水平分别检测热休克蛋白90和凋亡抑制蛋白Livin在病理性瘢痕中的表达情况及两者之间的关系。
方法：分别采用RT-PCR方法和免疫组织化学方法检测热休克蛋白90和Livin在24例瘢痕疙瘩、26例增生性瘢痕、22例非病理性瘢痕和20例正常皮肤组织中的表达水平。
结果与结论：瘢痕疙瘩，增生性瘢痕中热休克蛋白90和Livin的阳性表达水平高于非病理性瘢痕及正常皮肤组织(P < 0.05)。病理性瘢痕中热休克蛋白90和Livin表达呈正相关(rs=0.436, P < 0.05)。RT-PCR检测结果与免疫组织化学结果基本一致。结果提示热休克蛋白90和Livin在病理性瘢痕的形成过程中可能起着重要作用，且二者之间可能具有协同作用。
 

关键词: 热休克蛋白90, Livin, 病理性瘢痕, 反转录聚合酶链式反应, 免疫组织化学, 凋亡

Abstract:

BACKGROUND: In recent years, related research has shown that heat shock protein 90 (HSP90) and inhibitor of apoptosis protein Livin play a very important role in the process of cell apoptosis. However, the roles of HSP90 and Livin in the formation of pathologic scars are rarely reported.
OBJECTIVE: To investigate the expression and correlation of HSP90 and Livin in pathologic scars in gene and protein levels.
METHODS: Reverse transcription-PCR and SP immunohistochemical methods were used to detect the expressions of HSP90 and Livin in 24 cases of keloid, 26 cases of hypertrophic scar, 22 cases of non-pathologic scar and 20 cases of normal skin tissue.
RESULTS AND CONCLUSION: The positive levels of HSP90 and Livin in the keloid group and hypertrophic scar group were higher than that in the non-pathologic scar group and normal skin group (P < 0.05). There was a positive correlation between HSP90 and Livin expressions in the pathologic scars (rs=0.436, P < 0.05). RT-PCR results were similar to the immunohistochemical results. The results suggest that HSP90 and Livin may play an important role in the formation of pathologic scar; and there may be a synergistic effect between the two.
Wang DW, Wang XM, Liu LB, Wang QY, Peng Q, Zhang J, Sun YF, Zhang QG. Expression of heat shock protein 90 and inhibitor of apoptosis protein Livin in pathologic scars. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(15): 2709-2714.     [http://www.crter.cn  http://en.zglckf.com]

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