中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (13): 2362-2365.doi: 10.3969/j.issn.1673-8225.2012.13.019

• 脊柱损伤基础实验 basic experiments of spinal injury • 上一篇    下一篇

石膏外固定修复兔髌骨-腱连接点断裂的组织形态学变化★

王  靖1，张朝跃2，谭财福3   

  1. 1湖南省人民医院骨科，湖南省长沙市  410005；2 中南大学湘雅三医院骨科，湖南省长沙市  410013；  3株洲市中医伤科医院骨科，湖南省株洲市  412007
  • 收稿日期:2011-12-09 修回日期:2012-02-01 出版日期:2012-03-25 发布日期:2012-03-25
  • 通讯作者: 张朝跃，教授，博士生导师，中南大学湘雅三医院骨科，湖南省长沙市 410013 zcy1958@medmail.com
  • 作者简介:王靖★，男，1976年生，湖南省邵阳市人，汉族，硕士，主治医师，主要从事关节外科、运动医学的研究。 wjzj930@yahoo.com.cn

Effect of plaster external fixation on the histomorphological changes for bone-tendon junction healing

Wang Jing1, Zhang Chao-yue2, Tan Cai-Fu3   

  1. 1Department of Orthopedics, Hunan Provincial People’s Hospital, Changsha  410005, Hunan Province, China; 2Department of Orthopedics, the Third Xiangya Hospital of Central South University, Changsha  410013, Hunan Province, China; 3Deprtment of Orthopedics, Zhuzhou TCM Traumatic Hospital, Zhuzhou  412007, Hunan Province, China
  • Received:2011-12-09 Revised:2012-02-01 Online:2012-03-25 Published:2012-03-25
  • Contact: author: Zhang Chao-yue, Professor, Doctoral supervisor, Department of Orthopedics, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China zcy1958@medmail.com
  • About author:Wang Jing★, Master, Attending physician, Department of Orthopedics, Hunan Provincial People’s Hospital, Changsha 410005, Hunan Province, China wjzj930@yahoo.com.cn

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摘要：

背景：石膏外固定对髌骨-腱连接点断裂的治疗作用肯定，但相关修复机制一直未有深入研究。
目的：观察石膏外固定对髌骨-腱连接点断裂修复的组织形态学改变。
方法：切断髌尖与髌腱连接处并切除远端部分髌骨建立兔髌骨-腱连接点损伤模型，实验组在造模的基础上给予石膏外固定，模型组不予固定，分别在造模后2，4，8周取材。
结果与结论：苏木精-伊红染色、Safranin’O染色和巨噬细胞免疫组化染色及其积分吸光度检测均显示实验组造模后骨-腱结合部组织修复快，软骨细胞及骨组织的形成、分化明显优于模型组，且炎症反应程度轻、消失快。说明外固定可加快骨-腱组织修复进程，促进其愈合。
关键词：骨-腱连接点；外固定；组织形态学；积分吸光度值
doi:10.3969/j.issn.1673-8225.2012.13.019

关键词: 骨-腱连接点, 外固定, 组织形态学, 积分吸光度值

Abstract:

BACKGROUND: Plaster external fixation has a positive effect on bone-tendon junction healing, but there is no in-depth study on the repair mechanism.
OBJECTIVE: To investigate the effect of plaster external fixation on the histomorphological changes for bone-tendon junction healing.
METHODS: The bone-tendon junction healing models were established by cutting off the junction between the patellar tip and the patellar tendon as well as the distal part of the patella. The models were divided into experimental group and model group. The models in the experimental group were performed with plaster external fixation and those in the model group were without fixation. Each group got the sample at 2, 4 and 8 weeks after operation.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining, Safranin’O staining, macrophages immunohistochemistry staining and integral absorbance showed that the tissue edema and adhesion at the bone-tendon junction in the experimental group were better than those in the model group, cartilage cells and bone tissue formation and differentiation in the experimental group were significantly better those those in the model group, and degree of the inflammation in the experimental group was light and disappeared quickly. External fixation can accelerate the process of bone-tendon repair and promote the healing. 
 

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